rhodopsin抗体 (1D4) 推荐用于 mouse, rat 和 human 来源的rhodopsin WB, IP, IF, IHC(P) 和 ELISA检测

rhodopsin 抗体 (1D4): sc-57432

rhodopsin抗体 (1D4) is rated 4.4 out of 5 by 11.
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说明书

    • 抗-rhodopsin 抗体 1D4 是小鼠单克隆 IgG1 (kappa轻链),rhodopsin抗体,规格为200 µg/ml
    • 免疫bovine来源的rhodopsin全长
    • 抗-rhodopsin 抗体 (1D4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 rhodopsin; 也和以下物种反应,包括: bovine
    • 抗-rhodopsin 抗体 (1D4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取rhodopsin (1D4): sc-57432免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Would this antibody recognize the 1D4 tag in fusion protein in western blot?

Asked by: rogomca
Thank you for your question. This is a mouse monoclonal antibody that is recommended for detection of the protein rhodopsin. The clone name for this antibody is “1D4”, which is also known as the “1D4” epitope of rhodopsin. We have not tested this antibody for detection of any 1D4 fusion proteins.
Answered by: Technical Support
Date published: 2019-08-17

Can this mouse monoclonal antibody be used in IF or IHC on mouse tissue without background or non-specific staining issues?

Asked by: Anonymous
Thank you for your question. Yes, it can be used successfully for this application. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody rhodopsin (1D4): sc-57432 is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 4 out of 5 by from Visualizing Endogenous Rhodopsin in RPE Cells Useful antibody for IF labeling of endogenous rhodopsin in RPE cells. Cells permeabilized for 30min in PBStween then blocked in 5%BSA. Antibody used at 1:200 overnight at 4 degrees.
Date published: 2020-02-14
Rated 4 out of 5 by from Antibody for IF I used this antibody on OCT embedded frozen retinal sections (mouse). Blocked for 1 hour with 10% goat serum buffer. Incubated with primary antibody overnight at the a concentration of 1:100. Incubated with secondary antibody (alexa fluor 568 anti-mouse) at a concentration of 1:1000. Staining was fairly robust but not completely consistent between all samples.
Date published: 2018-03-21
Rated 2 out of 5 by from Poor specificity in normal canine WB Antibody was used for WB with normal canine tissue. Very low specificity.
Date published: 2017-08-18
Rated 5 out of 5 by from Great reactivity. This antibody will give you strong and specific signals in your blotting and staining. It even cross-reacts with additional species. It has been published with over 15 times.
Date published: 2017-01-11
Rated 5 out of 5 by from Outstanding!! Great product for both western blot and ihc cell staining applications
Date published: 2017-01-05
Rated 4 out of 5 by from Decent I bought this antibody for WB of rhodopsin in Y79 cells. So far it works as advertised.
Date published: 2016-12-12
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background in mouse eye and rat eye tissue extracts. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human HEK 293 and ARPE-19 cells - SCBT Publication Review
Date published: 2015-04-04
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