m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for RGS4 Antibody (H-12) for WB applications. This reagent is now offered in a bundle with RGS4 Antibody (H-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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RGS4 Antibody (H-12) is a high quality monoclonal RGS4 antibody (also designated RGP4 antibody) suitable for the detection of the RGS4 protein of mouse, rat and human origin. RGS4 Antibody (H-12) is available as the non-conjugated anti-RGS4 antibody. Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four Gα GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Gα-interacting protein). Each of these proteins has been shown to deactivate specific Gα isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Gα i, Gα o and Gα t, but not Gα s. RGS10 increases the GTP hydrolytic activity of several members of the Gα i subfamily including Gα i-3, Gα z and Gα o.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What application is the blocking peptide sc-398348 P appropriate for?
Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
Works great in WBI got this sample quite while. Several times' WB indicate the antibody works well in muscle myoblasts.
Date published: 2017-10-20
Rated 2 out of
Does not seem to recognize the main form of RGS4We received a sample of this antibody and it recognized a band around 36 kDa, which is not the predicted molecular weight, nor where another antibody detects RGS4. Furthermore, the intensity of this band did not change with modulation of a negative regulator of RGS4. We did not confirm the authenticity of ~36 kDa band with any type of knockdown, however.
Date published: 2017-06-16
Rated 5 out of
Very good Western blot data of RGS4 expressionVery good Western blot data of RGS4 expression in SK-N-SH, SK-N-MC, Raji, MDA-MB-231 and Jurkat whole cell lysates. -SCBT QC