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RASSF1 Antibody (3F3) - Western Blotting - Image 40024
RASSF1 抗体 (3F3): sc-58470. 对 HeLa 全细胞裂解液中 RASSF1 的表达进行 Western 印迹分析.
RASSF1 Antibody (3F3) - Western Blotting - Image 40024
RASSF1 抗体 (3F3): sc-58470. 对 HeLa 全细胞裂解液中 RASSF1 的表达进行 Western 印迹分析.
RASSF1 抗体 (3F3): sc-58470. 对 HeLa 全细胞裂解液中 RASSF1 的表达进行 Western 印迹分析.

RASSF1 抗体 (3F3): sc-58470

4.2(5)
寫評論提問

说明书
  • RASSF1抗体(3F3)是小鼠单克隆IgG1 κ, 在20篇文献中引用，规格为50 µg/500 µl
  • 针对与RASSF1的氨基酸1-340（human来源）相对应的重组蛋白，表位定位在C1域（52-101）
  • 推荐用于 mouse, rat 和 human 来源的RASSF1 WB, IP 和 IF检测
  • 关于如何获取RASSF1 (3F3): sc-58470的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成RASSF1 抗体 (3F3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 RASSF 抗体 包括 RASSF1, RASSF2, RASSF3, RASSF7和RASSF8

RASSF1抗体（3F3）是一种IgG1 κ小鼠单克隆RASSF1抗体，可通过WB、IP和IF检测小鼠、大鼠和人类的RASSF1。RASSF1抗体（3F3）可作为非偶联抗RASSF1抗体形式提供。活化的Ras蛋白可诱导衰老、凋亡和终末分化，尽管它们通常与刺激生长和转化有关。Ras关联结构域家族1（RASSF1）基因位于人类肺肿瘤抑制基因位点3p21.3。它由两个主要的可选择性转录物组成，RASSF1A和RASSF1C。在体内和体外，RASSF1均以GTP依赖的方式与Ras结合。活化的Ras增强，而显性负性Ras抑制由RASSF1瞬时转染到293-T细胞所诱导的细胞死亡，这表明RASSF1肿瘤抑制因子可能作为Ras效应物，介导致癌Ras的凋亡效应。RASSF1A通过其启动子区域CpG island的过度甲基化，在肺癌和乳腺癌中发生表观遗传失活。突变型RASSF1A的生长抑制活性显著降低。因此，RASSF1A是一个潜在的肿瘤抑制基因，在多种肿瘤的发病机制中起着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RASSF1 抗体 (3F3) 参考文献:

  1. 肺部肿瘤抑制基因座 3p21.3 中 RAS 缔合作用域家族蛋白的表观遗传失活。  |  Dammann, R., et al. 2000. Nat Genet. 25: 315-9. PMID: 10888881
  2. Ras 利用新型肿瘤抑制因子 RASSF1 作为介导细胞凋亡的效应因子。  |  Vos, MD., et al. 2000. J Biol Chem. 275: 35669-72. PMID: 10998413
  3. 肺癌, 乳腺癌和卵巢癌中 3p21.3 区域 RASSF1A 的甲基化缔合作用失活。  |  Agathanggelou, A., et al. 2001. Oncogene. 20: 1509-18. PMID: 11313894
  4. 肺癌和乳腺癌中 RASSF1A 的表观遗传失活及恶性表型抑制。  |  Burbee, DG., et al. 2001. J Natl Cancer Inst. 93: 691-9. PMID: 11333291
  5. 人类染色体 3p21.3 上的候选抑癌基因 RASSF1A 与肾脏肿瘤发生有关。  |  Dreijerink, K., et al. 2001. Proc Natl Acad Sci U S A. 98: 7504-9. PMID: 11390984
  6. 新型肿瘤抑制基因 RASSF1A 的 CpG 岛在原发性小细胞肺癌中被强烈甲基化。  |  Dammann, R., et al. 2001. Oncogene. 20: 3563-7. PMID: 11429703
  7. 胰腺导管腺癌中的 RASSF1 肿瘤抑制基因：表达、染色体状态和表观遗传学变化的相关性。  |  Amato, E., et al. 2016. BMC Cancer. 16: 11. PMID: 26754001
  8. 上皮性卵巢癌中的 EGFL7 和 RASSF1 启动子高甲基化。  |  Rattanapan, Y., et al. 2018. Cancer Genet. 224-225: 37-40. PMID: 29778234
  9. RASSF1-AS1是RASSF1A的反义lncRNA，它能抑制RASSF1A的翻译，从而加剧小鼠的心脏纤维化。  |  Guo, M., et al. 2019. Cell Biol Int. 43: 1163-1173. PMID: 30571844
  10. 高甲基化的RASSF1和SLC5A8启动子与BRAFV600E突变一起作为甲状腺乳头状癌的生物标志物。  |  Khatami, F., et al. 2020. J Cell Physiol. 235: 6954-6968. PMID: 32017063
  11. RASSF1 甲基化在肺癌中的作用  |  Zhang, T., et al. 2020. Adv Exp Med Biol. 1255: 99-108. PMID: 32949393
  12. 通过转录组协调分析，RASSF1 被确定为 ATF4 的靶标。  |  Zhang, Y., et al. 2023. FEBS Open Bio. 13: 556-569. PMID: 36723232

订购信息

产品名称产品编号规格价格数量收藏夹

RASSF1 抗体 (3F3)

sc-58470
50 µg/500 µl
$316.00

Can this RASSF1 Antibody (3F3): sc-58470 use for IHC and IF staining?

Asked by: Medhi
Thank you for your question. We recommend sc-58470 for WB, IP, and IF.
Answered by: BlakeJ
Date published: 2023-03-16

what is the different between the antibody of RASSF1 and RASSF1A?

Asked by: amorino
Thank you for your question.RASSF1A is a subtype of RASSF1. If you have any further questions, you could email us directly at asia@scbio.cn. we can have a more interactive discussion about this and other related issues. 谢谢你的问题。RASSF1A是RASSF1蛋白的一个亚型蛋白。如果您还有其他问题，您可以发邮件至asia@scbio.cn。我们可以就这个问题和其他相关问题进行更详细的讨论。
Answered by: Zhanglong
Date published: 2023-02-02

What is the amino acid sequence？

Asked by: amorimo
Thank you for your question. RASSF1 (3F3): sc-58470 is raised against a recombinant protein corresponding to amino acids 1-340 of RASSF1 of human origin, with epitope mapping to the C1 domain (52-101). Please contact Technical Service if you have further questions concerning this product. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2023-02-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 10
Date published: 2017-01-17
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Rated 3 out of 5 by from The band is a lillte weak1:200 diluted for wb of rat protein,the band is a little weak
Date published: 2018-03-21
Rated 5 out of 5 by from Good choice for WBRASSF1 Antibody (3F3) produced specific and clear band with HeLa whole cell lysate
Date published: 2017-01-24
Rated 5 out of 5 by from Nice publishable data on Western blot onNice publishable data on Western blot on various human samples. -SCBT Publication Review
Date published: 2015-06-16
Rated 4 out of 5 by from Works well for use in Western Blot and ImmunofluorescenceWorks well for use in Western Blot and Immunofluorescence using transfected COS-7 cells.
Date published: 2015-05-11
Rated 4 out of 5 by from Band detected at expected molecular weightBand detected at expected molecular weight in HeLa whole cell lysate. -SCBT QC
Date published: 2013-05-24
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RASSF1 抗体 (3F3) is rated 4.2 out of 5 by 5.
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