Ras2 Antibody (A-11): sc-365773

Ras2 Antibody (A-11) is a mouse monoclonal IgG3 antibody that detects Ras2 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ras2 antibody (A-11) is available as a non-conjugated format, providing researchers with a reliable tool for studying this critical protein. Ras2 protein plays a vital role in the regulation of cell growth and development in yeast by cycling between an active GTP-bound state and an inactive GDP-bound state, which is essential for proper signal transduction. This cycling is influenced by guanine nucleotide exchange factors, such as Cdc25, which modulate Ras2 and Ras1 activity. Ras2 activity is crucial for the regulation of adenylyl cyclase, an enzyme encoded by the Cdc35 gene, which catalyzes the production of cyclic adenosine monophosphate (cAMP), a key second messenger in various cellular processes. cAMP, in turn, activates a cAMP-dependent protein kinase composed of regulatory and catalytic subunits, ultimately influencing numerous downstream signaling pathways. Understanding Ras2 function and regulation is important for elucidating the mechanisms of cell signaling and growth in yeast, making Ras2 (A-11) antibody an invaluable asset for researchers in the field.

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Ras2 Antibody (A-11) References:

1. DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase.  |  Kataoka, T., et al. 1985. Cell. 43: 493-505. PMID: 2934138
2. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.  |  Toda, T., et al. 1987. Cell. 50: 277-87. PMID: 3036373
3. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae.  |  Toda, T., et al. 1987. Mol Cell Biol. 7: 1371-7. PMID: 3037314
4. The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway.  |  Broek, D., et al. 1987. Cell. 48: 789-99. PMID: 3545497
5. Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins.  |  Broek, D., et al. 1985. Cell. 41: 763-9. PMID: 3891097
6. Interactions between adenylyl cyclase, CAP and RAS from Saccharomyces cerevisiae.  |  Mintzer, KA. and Field, J. 1994. Cell Signal. 6: 681-94. PMID: 7531994
7. Inactivation of the CDC25 gene product in Saccharomyces cerevisiae leads to a decrease in glycolytic activity which is independent of cAMP levels.  |  Oehlen, LJ., et al. 1993. J Gen Microbiol. 139: 2091-100. PMID: 8245836

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ras2 Antibody (A-11) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ras2 Antibody (A-11) Specifications

Alternate Product Name: Ras2

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids: 279-312

Epitope Target: Ras2

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Ras2

Species Reactivity/Detection: y

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ras2 Antibody (A-11): P: Blocking Peptide