Rap 2A/B/C抗体 (C-2) 推荐用于 mouse, rat 和 human 来源的Rap 2A, Rap 2B and Rap 2C WB, IP, IF 和 ELISA检测

Rap 2A/B/C 抗体 (C-2): sc-515711

Rap 2A/B/C抗体 (C-2) is rated 5.0 out of 5 by 1.
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说明书

    • Rap 2A/B/C抗体(C-2)是小鼠单克隆IgG2a (kappa轻链),规格为200 µg/ml
    • 免疫human物种的的氨基酸1-183
    • 抗-Rap 2A/B/C 抗体 (C-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Rap 2A, Rap 2B and Rap 2C
    • 抗-Rap 2A/B/C 抗体 (C-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Rap 2A/B/C (C-2): sc-515711免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Rap 2A/B/C Antibody (C-2) for WB applications. This reagent is now offered in a bundle with Rap 2A/B/C Antibody (C-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

For Western Blot, is it recommended to use denatured or non-denatured conditions with Rap 2A/B/C (C-2): sc-515711 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Testing Ra2A/B/C antibody in BREC lysates The Rap2A/B/C (sc-515711) antibody works well in bovine cell lysates using 5% milk as blocking solution. The band is at 20 kD. Note that there is an additional band at 50 kD this might be do to dimerization of the Rap proteins.
Date published: 2017-10-04
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