Date published: 2025-9-7
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Ran BP-2 Antibody (D-4) - Immunofluorescence - Image 131801
Ran BP-2 抗体 (D-4): sc-74518. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核膜定位.
Ran BP-2 抗体 (D-4): sc-74518. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核膜定位.

Ran BP-2 抗体 (D-4): sc-74518

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寫評論提問

说明书
  • Ran BP-2 抗体 D-4 是小鼠单克隆 IgG1 κ，Ran BP-2抗体, 在33篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-300，在Ran BP-2的human起源的N-terminus处进行映射
  • Ran BP-2 抗体 (D-4) 推荐用于 WB, IP, IF 和 ELISA，检测 Ran BP-2 of mouse, rat and human origin, and the RGPD family of proteins of human origin
  • 抗Ran BP-2抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ran BP-2 (D-4): sc-74518的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Ran BP-2 Antibody (D-4) for WB applications. 的首选辅助检测试剂，这些试剂现与Ran BP-2 Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Ran BP抗体，包括Ran BP-1, Ran BP-2, Ran BP-3和Ran BP-M

Ran BP-2抗体（D-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光 （IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Ran BP-2蛋白。抗Ran BP-2抗体（D-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Ran BP-2蛋白（也称为Nup358）在核质运输中起着至关重要的作用，它充当与小GTP酶Ran相互作用的支架，而Ran对于RNA和蛋白质通过核孔复合物的运输至关重要。这种相互作用通过调节DNA复制和有丝分裂过程中的分子进出来维持细胞内环境稳定。Ran BP-2基因位于2q染色体上的基因组区域，与多种遗传疾病有关，包括少年肾小球肾炎和先天性肝纤维化，这凸显了其在人类健康和疾病中的重要性。Ran BP-2蛋白中的四个RanBD1结构域通过促进与Ran-GTP酶的相互作用，增强了细胞运输机制，从而凸显了结构复杂性和功能重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ran BP-2 抗体 (D-4) 参考文献:

  1. RanBP2 的锌指簇结构域是核输出因子 exportin-1 的特定对接位点。  |  Singh, BB., et al. 1999. J Biol Chem. 274: 37370-8. PMID: 10601307
  2. Ran 与来自 RanBP2 和 importin-beta 的 Ran 结合域相互作用的不同结构和动力学要求。  |  Villa Braslavsky, CI., et al. 2000. Biochemistry. 39: 11629-39. PMID: 10995230
  3. 小鼠 Ran 结合蛋白 2（RanBP2）基因的基因组结构, 表达和定位。  |  Fauser, S., et al. 2001. Mamm Genome. 12: 406-15. PMID: 11353387
  4. 人类 2 号和 4 号染色体 DNA 序列的生成和注释。  |  Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
  5. 急性髓单核细胞白血病中RANBP2-ALK融合合并7单体。  |  Lim, JH., et al. 2014. Cancer Genet. 207: 40-5. PMID: 24613277
  6. 拟南芥与叶绿体 RNA 编辑因子 OZ1 相关的 RanBP2 型锌指蛋白  |  Gipson, AB., et al. 2020. Plants (Basel). 9: PMID: 32121603
  7. 与 RANBP2 有关的遗传性急性坏死性脑病：对儿科临床和治疗的影响。  |  Levine, JM., et al. 2020. Mult Scler Relat Disord. 43: 102194. PMID: 32426208
  8. 核蛋白 RanBP2/Nup358 在 1 型急性坏死性脑病 (ANE1) 和病毒感染中的作用  |  Jiang, J., et al. 2022. Int J Mol Sci. 23: PMID: 35408907
  9. RanBP2/Nup358与急性坏死性脑病研讨会。  |  Palazzo, AF., et al. 2022. Nucleus. 13: 154-169. PMID: 35485383
  10. RanBP2/Nup358 介导 STAT1 的 Sumoylation 并拮抗干扰素-α介导的抗病毒先天免疫力  |  Li, J., et al. 2023. Int J Mol Sci. 25: PMID: 38203469
  11. 一种参与将 RanGAP1 靶向核孔复合体蛋白 RanBP2 的泛素相关小多肽。  |  Mahajan, R., et al. 1997. Cell. 88: 97-107. PMID: 9019411
  12. RanGTP 将 p97 靶向 RanBP2，RanBP2 是一种定位于核孔复合体细胞质外围的丝状蛋白。  |  Delphin, C., et al. 1997. Mol Biol Cell. 8: 2379-90. PMID: 9398662

订购信息

产品名称产品编号规格价格数量收藏夹

Ran BP-2 抗体 (D-4)

sc-74518
200 µg/ml
$316.00

Ran BP-2 (D-4): m-IgG Fc BP-HRP 套装

sc-527036
200 µg Ab; 10 µg BP
$354.00

Ran BP-2 (D-4): m-IgG1 BP-HRP 套装

sc-532409
200 µg Ab; 20 µg BP
$354.00

Ran BP-2 抗体 (D-4) AC

sc-74518 AC
500 µg/ml, 25% agarose
$416.00

Ran BP-2 抗体 (D-4) HRP

sc-74518 HRP
200 µg/ml
$316.00

Ran BP-2 抗体 (D-4) FITC

sc-74518 FITC
200 µg/ml
$330.00

Ran BP-2 抗体 (D-4) PE

sc-74518 PE
200 µg/ml
$343.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 488

sc-74518 AF488
200 µg/ml
$357.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 546

sc-74518 AF546
200 µg/ml
$357.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 594

sc-74518 AF594
200 µg/ml
$357.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 647

sc-74518 AF647
200 µg/ml
$357.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 680

sc-74518 AF680
200 µg/ml
$357.00

Ran BP-2 抗体 (D-4) Alexa Fluor® 790

sc-74518 AF790
200 µg/ml
$357.00

Are there any issues with background when using this antibody to stain mouse tissues?

Asked by: AbPolly
To avoid potential background staining, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Service 11
Date published: 2017-01-12

What is the recommended secondary for Western Blot when using this antibody?

Asked by: AbPolly
We recommend using our Mouse IgG kappa light chain binding protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-12
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Rated 2 out of 5 by from 不太满意不知道是不是小样与正装存在差异，第一次使用条带比较清晰，无法回收二次使用；且之后重新配制条带也不是很清晰，出现异常杂带
Date published: 2022-03-11
Rated 5 out of 5 by from Very good for IFThis antibody gives very good staining on paraformaldehyde fixed cells, at the nuclear envelope as it should be for the Ran-BP protein
Date published: 2019-02-01
Rated 4 out of 5 by from Bom para WBPedi uma amostra do Ran BP-2 (D-4) e funcionou bem no Western Blot para a transfecção da proteina
Date published: 2018-02-07
Rated 4 out of 5 by from Good for WBit works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Hela cells, RanBP2 IP, RanBP2 blotHi Aanta Cruz Biotechnology, I tried Ran BP-2 Antibody (D-4) for Western Blotting of cell lysate from HeLa cells. The blot is clear. I think the antibody is good. Thank you so much for your antibody sample. Best, Wenli Yang UTHealth
Date published: 2017-09-11
Rated 5 out of 5 by from excellant！I use it for IF.The result is very good！I can see the protein near the nuclar clearly.
Date published: 2017-09-06
Rated 4 out of 5 by from Good for IFUsed this Ran-BP2 ab on paraformaldehyde fixed HEK293T cells at 1:100 concentration and the staining looks very nice.
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody.Produced good immunofluorescence nuclear membrane staining in PFA-fixed RCTE cells. Dilution 1:100.
Date published: 2017-06-22
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Ran BP-2 抗体 (D-4) is rated 4.5 out of 5 by 13.
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