Date published: 2025-9-6

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Ral A 抗体 (F-5): sc-374462

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说明书
  • Ral A 抗体 F-5 是小鼠单克隆 IgG2b κ,Ral A抗体, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Ral A的C-terminus的氨基酸181-209之间
  • Ral A 抗体 (F-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ral A
  • 抗Ral A抗体(F-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ral A (F-5): sc-374462免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Ral A 抗体 (F-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ral A 抗体 (F-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ral A抗体(F-5)是一种IgG2bκ小鼠单克隆Ral A抗体(也称为Ral A抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Ral A蛋白。Ral A抗体(F-5)既可作为非偶联的抗Ral A抗体形式提供,也可作为多种偶联形式的抗Ral A抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ral A和Ral B构成Ras相关GTP酶(即GDP/GTP结合蛋白)的一个独特的亚家族。Ral蛋白由独特的核苷酸交换因子Ral GDS激活,并由独特的GTP酶激活蛋白失活。与Ras蛋白不同,当在各种受体细胞中表达活化变体时,Ral A和Ral B未能诱导转化病灶。已显示被命名为Ral BP-1的Ral的潜在下游靶标包含Rho-GTP酶激活结构域。该Rho-GTP酶激活结构域优先与Rho家族成员Cdc42相互作用。据报道,Ras/Ral信号通路介导v-Src对磷脂酶D(PLD)的激活,从而表明PLD是Ral A的另一个下游靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ral A 抗体 (F-5) 参考文献:

  1. Ral GAP复合物将PI 3-激酶/Akt信号传导与胰岛素作用中的RalA激活联系起来。  |  Chen, XW., et al. 2011. Mol Biol Cell. 22: 141-52. PMID: 21148297
  2. 系统泛癌分析确定RALA作为肿瘤靶向免疫治疗和预后标记物。  |  Jin, H., et al. 2022. Front Immunol. 13: 1046044. PMID: 36466919
  3. Reps1-Ralbp1-RalA模块对货物外吞的调控。  |  Wang, S., et al. 2023. Sci Adv. 9: eade2540. PMID: 36812304
  4. 确定RALA作为骨肉瘤的治疗靶点和预后预测因子。  |  Fan, G., et al. 2023. Biomed Res Int. 2023: 1150768. PMID: 36817861
  5. 高脂饮食和线粒体形状之间的RalA。  |  Zambello, L. and Scorrano, L. 2024. Nat Metab. 6: 198-199. PMID: 38286820
  6. 肥胖导致线粒体断裂和白细胞脂肪细胞功能障碍,这是由于RalA激活所致。  |  Xia, W., et al. 2024. Nat Metab. 6: 273-289. PMID: 38286821
  7. Ral GTPase参与v-Src诱导的磷脂酶D激活过程  |  Jiang, H., et al. 1995. Nature. 378: 409-12. PMID: 7477381
  8. Ral 结合蛋白 1(Ral GTPases 的潜在下游靶标)的鉴定和表征。  |  Cantor, SB., et al. 1995. Mol Cell Biol. 15: 4578-84. PMID: 7623849
  9. 连接 Ral GTPase 与 Rho 通路。RLIP76是一种具有CDC42/Rac GTP酶激活蛋白活性的Ral效应因子。  |  Jullien-Flores, V., et al. 1995. J Biol Chem. 270: 22473-7. PMID: 7673236
  10. 大鼠 ral A 和 ral B GTP 结合蛋白的 cDNA 克隆的分离和组织表达。  |  Wildey, GM., et al. 1993. Biochem Biophys Res Commun. 194: 552-9. PMID: 7687439
  11. 鉴定鸟嘌呤核苷酸解离刺激物 Ral 为 R-ras, H-ras, K-ras 和 Rap 的假定效应分子。  |  Spaargaren, M. and Bischoff, JR. 1994. Proc Natl Acad Sci U S A. 91: 12609-13. PMID: 7809086
  12. 活化的 Ras 与 Ral 鸟嘌呤核苷酸解离刺激因子相互作用。  |  Hofer, F., et al. 1994. Proc Natl Acad Sci U S A. 91: 11089-93. PMID: 7972015

订购信息

产品名称产品编号规格价格数量收藏夹

Ral A 抗体 (F-5)

sc-374462
200 µg/ml
$316.00

Ral A (F-5): m-IgG Fc BP-HRP 套装

sc-529749
200 µg Ab; 10 µg BP
$354.00

Ral A (F-5): m-IgGκ BP-HRP 套装

sc-522746
200 µg Ab, 40 µg BP
$354.00

Ral A (F-5): m-IgG2b BP-HRP 套装

sc-550140
200 µg Ab; 10 µg BP
$354.00

Ral A 抗体 (F-5) AC

sc-374462 AC
500 µg/ml, 25% agarose
$416.00

Ral A 抗体 (F-5) HRP

sc-374462 HRP
200 µg/ml
$316.00

Ral A 抗体 (F-5) FITC

sc-374462 FITC
200 µg/ml
$330.00

Ral A 抗体 (F-5) PE

sc-374462 PE
200 µg/ml
$343.00

Ral A 抗体 (F-5) Alexa Fluor® 488

sc-374462 AF488
200 µg/ml
$357.00

Ral A 抗体 (F-5) Alexa Fluor® 546

sc-374462 AF546
200 µg/ml
$357.00

Ral A 抗体 (F-5) Alexa Fluor® 594

sc-374462 AF594
200 µg/ml
$357.00

Ral A 抗体 (F-5) Alexa Fluor® 647

sc-374462 AF647
200 µg/ml
$357.00

Ral A 抗体 (F-5) Alexa Fluor® 680

sc-374462 AF680
200 µg/ml
$357.00

Ral A 抗体 (F-5) Alexa Fluor® 790

sc-374462 AF790
200 µg/ml
$357.00

Ral A (F-5) 中和勝肽

sc-374462 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-374462 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Doesn't work for IFI tried using this antibody at 1:100 with 2 different permeabilization conditions : 1. 0.25% Triton-X-100 and 2. 0.1% saponinin. Neither of these conditions worked. Antibody appeared non-specific
Date published: 2017-11-18
Rated 5 out of 5 by from Ral A Antibody (F-5)We have done in the fish cells related experiments, the band is not very obvious, ready to try to change the secondary antibody concentration and then try. thank you very much
Date published: 2017-04-17
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Produced positive Western Blot data of RalProduced positive Western Blot data of Ral A expression in non-transfected and human Ral A transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-06
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Ral A 抗体 (F-5) is rated 4.0 out of 5 by 4.
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