Date published: 2025-9-6

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Rab 7 抗体 (B-3): sc-376362

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说明书
  • Rab 7 抗体 B-3 是小鼠单克隆 IgG1 κ,Rab 7抗体, 在83篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列158-207位于human物种的Rab 7的C-terminus
  • Rab 7 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Rab 7
  • 抗Rab 7抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 7 (B-3): sc-376362免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Rab 7 Antibody (B-3) for WB applications. 的首选辅助检测试剂,这些试剂现与Rab 7 Antibody (B-3) 打包提供(请参阅下面的订购信息)。

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関連項目

Rab 7抗体(B-3)是一种IgG1 κ小鼠单克隆Rab 7抗体(也称为RAB7抗体或Ras相关蛋白Rab-7a抗体),可通过WB, IP, IF和ELISA检测小鼠、大鼠和人类来源的Rab 7蛋白。Rab 7抗体(B-3)既有非偶联形式的抗Rab 7抗体,也有多种偶联形式的抗Rab 7抗体,包括琼脂糖、HRP、PE、FITC以及多种Alexa Fluor®偶联物。包括Ral/Rec、Rap、R-Ras和Rho/Rab亚家族在内的Ras相关鸟嘌呤核苷酸结合蛋白超家族与Ras p21具有30-60%的同源性。累积的数据表明,Rab蛋白在内吞作用或生物合成蛋白转运中发挥着重要作用。新合成的蛋白质从内质网运输到高尔基复合体的各个堆叠部位,再运输到分泌囊泡,这一过程的每个阶段都涉及载体囊泡的运动,这一过程似乎涉及Rab蛋白的功能。在酵母中,与Rab蛋白具有40%同源性的Sec4蛋白与分泌囊泡相关,这一观察结果支持Rab蛋白也可能指导分泌囊泡向质膜的胞吐作用的可能性。已经确定了Rab亚家族的几个成员,每个成员都位于膜转运途径的特定阶段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rab 7 抗体 (B-3) 参考文献:

  1. 通过 PCR 克隆方法揭示 Rab 和 Rho GTP 结合蛋白亚家族的复杂性。  |  Chavrier, P., et al. 1992. Gene. 112: 261-4. PMID: 1555775
  2. 克隆主要在脂肪细胞中表达的 Rab3 同工型。  |  Baldini, G., et al. 1992. Proc Natl Acad Sci U S A. 89: 5049-52. PMID: 1594612
  3. 人类 Rab 基因编码的 GTP 结合蛋白家族与参与分泌的酵母 YPT1 和 SEC4 产物有关。  |  Zahraoui, A., et al. 1989. J Biol Chem. 264: 12394-401. PMID: 2501306
  4. 重新审视哺乳动物自噬中的Rab7功能:Rab7基因敲除研究。  |  Kuchitsu, Y. and Fukuda, M. 2018. Cells. 7: PMID: 30463228
  5. Timosaponin AIII通过增强结肠直肠癌细胞中Rab7介导的脂质降解诱导脂质过氧化和铁死亡。  |  Shen, C., et al. 2024. Phytomedicine. 122: 155079. PMID: 37863004
  6. 夏科-玛丽牙齿病2B型Rab7突变感觉周围神经元线粒体-溶酶体接触动态的失调。  |  Wong, YC., et al. 2023. Proc Natl Acad Sci U S A. 120: e2313010120. PMID: 37878717
  7. Rab7 和 Rab9 通过生化不同的过程被招募到晚期内体上。  |  Soldati, T., et al. 1995. J Biol Chem. 270: 25541-8. PMID: 7592724
  8. 通过表位标签对 Rab8 和 Rab10 这两种低分子量 GTP 结合蛋白进行表达和定域。  |  Chen, YT., et al. 1993. Proc Natl Acad Sci U S A. 90: 6508-12. PMID: 7688123
  9. 朋友和家人:Rab GTPases 在囊泡交通中的作用。  |  Novick, P. and Brennwald, P. 1993. Cell. 75: 597-601. PMID: 8242735
  10. 鉴定人类血小板中的小 GTP 结合型 rab 蛋白:凝血酶诱导的 rab3B, rab6 和 rab8 蛋白磷酸化。  |  Karniguian, A., et al. 1993. Proc Natl Acad Sci U S A. 90: 7647-51. PMID: 8356066
  11. Rab 7:晚期内细胞膜交通的重要调节器。  |  Feng, Y., et al. 1995. J Cell Biol. 131: 1435-52. PMID: 8522602
  12. Rab11 是一种位于上皮组织顶端的小 GTP 结合蛋白。  |  Goldenring, JR., et al. 1996. Am J Physiol. 270: G515-25. PMID: 8638719

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 7 抗体 (B-3)

sc-376362
200 µg/ml
$316.00

Rab 7 (B-3): m-IgG Fc BP-HRP 套装

sc-527530
200 µg Ab; 10 µg BP
$354.00

Rab 7 (B-3): m-IgG1 BP-HRP 套装

sc-532903
200 µg Ab; 20 µg BP
$354.00

Rab 7 抗体 (B-3) AC

sc-376362 AC
500 µg/ml, 25% agarose
$416.00

Rab 7 抗体 (B-3) HRP

sc-376362 HRP
200 µg/ml
$316.00

Rab 7 抗体 (B-3) FITC

sc-376362 FITC
200 µg/ml
$330.00

Rab 7 抗体 (B-3) PE

sc-376362 PE
200 µg/ml
$343.00

Rab 7 抗体 (B-3) Alexa Fluor® 488

sc-376362 AF488
200 µg/ml
$357.00

Rab 7 抗体 (B-3) Alexa Fluor® 546

sc-376362 AF546
200 µg/ml
$357.00

Rab 7 抗体 (B-3) Alexa Fluor® 594

sc-376362 AF594
200 µg/ml
$357.00

Rab 7 抗体 (B-3) Alexa Fluor® 647

sc-376362 AF647
200 µg/ml
$357.00

Rab 7 抗体 (B-3) Alexa Fluor® 680

sc-376362 AF680
200 µg/ml
$357.00

Rab 7 抗体 (B-3) Alexa Fluor® 790

sc-376362 AF790
200 µg/ml
$357.00

does this antibody target at active Rab7-GTP? Can I use this antibody to localize late endosome for IF?

Asked by: wwww
Thank you for your question. This protein is classified as a GTPase. So, antibody detection to the Rab 7 would mean that this antibody undergoes GTPase binding. Hopefully this answers your question if not you can please provide more information on what you mean by active Rab 7 GTP.
Answered by: Ling Chen
Date published: 2024-06-11

does this antibody target at active Rab7-GTP? Can I use this antibody to localize late endosome?

Asked by: wwww
Thank you for your question. May I ask what type of immunological experimental method you would like to use this antibody for? WB, IF, or others?
Answered by: Ling Chen
Date published: 2024-05-31

I can not detect Rab7 in mouse motor cortex with this antibody. Have any of you tested in neuron primary culture?

Asked by: JáinaR
Thank you for your question. We have not tested this antibody in neuron primary culture.
Answered by: Technical Support
Date published: 2019-07-14

Can you recommend a positive control to use with Rab 7 Antibody (B-3): sc-376362 for Western blot?

Asked by: cjMara
We recommend C2C12 (sc-364188), 3T3-L1 (sc-2243), RAW 264.7 (sc-2211) or A549 (sc-2413) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of Rab 7 in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 5 out of 5 by from Rab7 antibody works well for IFMouse anti-Rab7 antibody works well for IF using T47D cells at 1:100.
Date published: 2024-02-26
Rated 5 out of 5 by from Excellent Ab in WBExcellent antibody in WB. In Hela cells it gave a strong unique band at 23 kDa, disappearing upon specific knockdown.
Date published: 2022-10-27
Rated 5 out of 5 by from western blotThis is a very nice antibody for my mouse primary neuron cells western blot. It is clean and strong!
Date published: 2022-08-30
Rated 5 out of 5 by from Good for IFマウスの培養細胞を用いてPFA固定後に免疫蛍光染色を行いました。エンドゾーム様の染色像を確認することができました。
Date published: 2020-08-24
Rated 5 out of 5 by from greatClear crisp bands, only one band, at predicted molecular weights
Date published: 2020-02-06
Rated 4 out of 5 by from worked great for IF for murine dendritic cellsworked great for IF for murine dendritic cells thank you
Date published: 2019-12-04
Rated 4 out of 5 by from Good antibodyThis antibody works well for WB in HeLa and COS7 cells (1:500)
Date published: 2019-06-19
Rated 4 out of 5 by from GOODIt worked well in 1:3000 in our cell lines, and I had pretty good signals
Date published: 2018-04-03
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Rab 7 抗体 (B-3) is rated 4.4 out of 5 by 30.
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