Date published: 2025-9-8

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Rab 7 抗体 (D-4): sc-271608

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说明书
  • Rab 7抗体(D-4)是小鼠单克隆IgG1 κ, 在11篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Rab 7的C-terminus的氨基酸173-204之间
  • 抗-Rab 7 抗体 (D-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Rab 7
  • 抗Rab 7抗体(D-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rab 7 (D-4): sc-271608免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Rab 7 Antibody (D-4) for WB applications. 的首选辅助检测试剂,这些试剂现与Rab 7 Antibody (D-4) 打包提供(请参阅下面的订购信息)。

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関連項目

Rab 7抗体(D-4)是一种IgG1 κ小鼠单克隆Rab 7抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Rab 7。Rab 7抗体(D-4)既可以是非偶联的抗Rab 7抗体形式,也可以是多种偶联形式的抗Rab 7抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。包括Ral/Rec、Rap、R-Ras和Rho/Rab亚家族在内的Ras相关鸟苷酸结合蛋白超家族与Ras p21具有30-60%的同源性。越来越多的数据表明,Rab蛋白在内吞作用或生物合成蛋白转运中发挥着重要作用。新合成的蛋白质从内质网转运到高尔基复合体的各个部位以及分泌囊泡的过程涉及每个阶段的载体囊泡运动,这一过程似乎与Rab蛋白的功能有关。在酵母中,与Rab蛋白有40%同源性的Sec4蛋白与分泌囊泡相关,这支持了Rab蛋白也可能直接指导分泌囊泡向质膜的胞吐作用的可能性。已经发现了Rab亚家族的几个成员,每个成员都位于膜转运途径的特定阶段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Rab 7 抗体 (D-4)

sc-271608
200 µg/ml
$316.00

Rab 7 (D-4): m-IgG Fc BP-HRP 套装

sc-527265
200 µg Ab; 10 µg BP
$354.00

Rab 7 (D-4): m-IgG1 BP-HRP 套装

sc-532638
200 µg Ab; 20 µg BP
$354.00

Rab 7 抗体 (D-4) AC

sc-271608 AC
500 µg/ml, 25% agarose
$416.00

Rab 7 抗体 (D-4) HRP

sc-271608 HRP
200 µg/ml
$316.00

Rab 7 抗体 (D-4) FITC

sc-271608 FITC
200 µg/ml
$330.00

Rab 7 抗体 (D-4) PE

sc-271608 PE
200 µg/ml
$343.00

Rab 7 抗体 (D-4) Alexa Fluor® 488

sc-271608 AF488
200 µg/ml
$357.00

Rab 7 抗体 (D-4) Alexa Fluor® 546

sc-271608 AF546
200 µg/ml
$357.00

Rab 7 抗体 (D-4) Alexa Fluor® 594

sc-271608 AF594
200 µg/ml
$357.00

Rab 7 抗体 (D-4) Alexa Fluor® 647

sc-271608 AF647
200 µg/ml
$357.00

Rab 7 抗体 (D-4) Alexa Fluor® 680

sc-271608 AF680
200 µg/ml
$357.00

Rab 7 抗体 (D-4) Alexa Fluor® 790

sc-271608 AF790
200 µg/ml
$357.00

Rab 7 (D-4) 中和勝肽

sc-271608 P
100 µg/0.5 ml
$68.00

Can I have this ample since you offered FREE 10 µg sample of Rab 7 (D-4): sc-271608

Asked by: Hilal
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Rab 7 Antibody (D-4) for Western blot. How do I know if the band I am detecting is specific?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the blocking peptide sc-271608 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 1 out of 5 by from Not really goodI briefly did western in various cancer cell lines, around 10ug lysate. The antibody can weakly detect the existence while products from Cell Signalling can be 10 times sensitive. I used 1/200 dilution. After checking molecular weight, it even does not recognize the right band.
Date published: 2019-03-14
Rated 5 out of 5 by from Great results in WBLittle background observed, band detected at right size. Used 1/1000 and blocked in 2% BSA
Date published: 2018-03-07
Rated 4 out of 5 by from Good antibody for WB!It works for WB with a very strong and specific band (35 KDa in Hela cells). Weak signal in IF (1/50 dilution in 1%BSA).
Date published: 2018-01-12
Rated 5 out of 5 by from Detect the faintest star in the galaxy of proteinsThis is an extraordinary antibody for detecting endocytic vesicle by Immunofluorescence. I have used the antibody (1:100) for detecting some late endosomal vesicles in a human carcinoma cell line and got satisfied by the specificity of the antibody and the clarity of the florescence signal (see the attached picture). Such a nice antibody, it will help you paint the endosomal vesicles with your choice of colour. So use it as long as you can and get good science out of it. Good luck with your research
Date published: 2017-04-10
Rated 5 out of 5 by from Great antibodyRab 7 Antibody (D-4) produced great IF and WB results.
Date published: 2017-01-12
Rated 5 out of 5 by from Strong signal seen in Western Blot with noStrong signal seen in Western Blot with no background -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from IF: methanolIF: methanol-fixed HeLa cells show cytoplasmic localization of signal -SCBT QC
Date published: 2014-12-08
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Rab 7 抗体 (D-4) is rated 4.3 out of 5 by 7.
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