QP-C CRISPR/Cas9 KO Plasmid (m): sc-423624
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- QP-C CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the QP-C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QP-C CRISPR/Cas9 KO Plasmid (m) | sc-423624 | 20 µg | $397.00 |
Overview
Uqcrq encodes QP-C, a small accessory subunit of mitochondrial respiratory chain complex III (ubiquinol–cytochrome c reductase) that supports assembly and stability of the cytochrome bc1 complex in the inner mitochondrial membrane. By influencing electron transfer from ubiquinol to cytochrome c, QP-C contributes to oxidative phosphorylation, mitochondrial membrane potential maintenance, and cellular redox balance. Disruption of complex III components can impair ATP production and elevate mitochondrial reactive oxygen species, linking Uqcrq-dependent processes to bioenergetic stress responses and apoptosis signaling. In mouse systems, Uqcrq is therefore relevant for mechanistic studies of mitochondrial dysfunction in metabolically active tissues and for modeling pathways implicated in neuromuscular and cardiometabolic phenotypes.
QP-C CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Uqcrq gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Uqcrq together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Uqcrq open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish QP-C protein expression.
This CRISPR knockout system enables efficient generation of Uqcrq-deficient cell models for investigation of QP-C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Uqcrq exon(s) critical for QP-C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Uqcrq genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by QP-C CRISPR/Cas9 KO Plasmid (m) and QP-C CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Uqcrq locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by QP-C HDR Plasmid (m) and QP-C HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Uqcrq homology arms to support homology-directed repair at defined Uqcrq target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.