Date published: 2025-9-10

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PU.1/Spi1 抗体 (A-7): sc-365208

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说明书
  • PU.1/Spi1抗体(A-7)是小鼠单克隆IgG1 κ, 在7篇文献中引用,规格为200 µg/ml
  • 免疫human物种的PU.1的氨基酸1-135
  • 推荐用于 human 来源的PU.1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-365208 X, 200 µg/0.1 ml)
  • 看 PU.1/Spi1 (C-3): sc-390405,可查看有标记的 PU.1/Spi1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 PU.1/Spi1 抗体 (A-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PU.1/Spi1 抗体 (A-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ets转录因子家族(包括Ets-1、Ets-2、Erg-1-3、Elk-1、Elf-1、Elf-5、NERF、PU.1、PEA3、ERM、FEV、ER81、Fli-1、TEL、Spi-B、ESE-1、ESE-3A、Net、ABT1和ERF)是一组DNA结合蛋白,它们影响淋巴的发育和活动。Ets家族的单体蛋白通过一个独特的带翼螺旋-转角-螺旋基序(称为Ets结构域)与共识DNA位点GGA(A/T)结合。PU.1(Spi-1/Spi-A)、Spi-B和Spi-C是密切相关的Ets家族成员,它们在Ets结构域内共享一个保守的发散序列,使它们能够与非规范的AGAA位点结合。PU.1反式激活大量B细胞基因,如编码CD72、CD20和Btk的基因,而Spi-B则增强许多这些相同靶基因的表达。PU.1在多种造血细胞中表达,包括B细胞、早期T细胞、巨核细胞、粒细胞、肥大细胞、未成熟红细胞和髓样细胞。相比之下,Spi-B的表达仅限于B细胞和未成熟的T细胞,在这些细胞中,表达通过T细胞谱系承诺而累积,然后在β选择检查点之后显著缺失。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

PU.1/Spi1 抗体 (A-7)

sc-365208
200 µg/ml
$316.00

PU.1/Spi1 (A-7): m-IgG Fc BP-HRP 套装

sc-540317
200 µg Ab; 10 µg BP
$354.00

PU.1/Spi1 (A-7): m-IgG1 BP-HRP 套装

sc-542038
200 µg Ab; 20 µg BP
$354.00

PU.1/Spi1 抗体 (A-7) X

sc-365208 X
200 µg/0.1 ml
$316.00

I'm looking for a PU.1 stain that will stain frozen mouse brain tissue - could you help? Preferably cross-reactive with Human.

Asked by: Nogs
Thanks for you question. Anti-PU.1 Antibody (C-3):sc-390405 is recommended for detection of PU.1 of mouse and human origin by IF, and IHC(P).
Answered by: Technical Support 9
Date published: 2020-06-12

I am using PU.1 (A-7): sc-365208 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background.
Date published: 2017-01-23
Rated 5 out of 5 by from Works well in WB and IP in different humanWorks well in WB and IP in different human cell lines.
Date published: 2015-12-14
Rated 5 out of 5 by from Very goodVery good!
Date published: 2015-03-16
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PU.1/Spi1 抗体 (A-7) is rated 5.0 out of 5 by 3.
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