Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

PSMB11 抗体 (H-1): sc-515132

5.0(1)
寫評論提問

说明书
  • PSMB11 抗体 H-1 是小鼠单克隆 IgG1 (kappa轻链),PSMB11抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的PSMB11的内部区域内的氨基酸113-130之间
  • PSMB11 抗体 (H-1) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 PSMB11
  • 抗PSMB11抗体(H-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PSMB11 (H-1): sc-515132免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是PSMB11 Antibody (H-1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与PSMB11 Antibody (H-1) 打包提供(请参阅下面的订购信息)。

    快捷链接

    関連項目

    PSMB11抗体(H-1)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测人源性PSMB11蛋白。PSMB11 (H-1) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。PSMB11,也称为蛋白酶体亚基β-11或BETA5T,是26S蛋白酶体的重要组成部分,26S蛋白酶体是一种多亚基蛋白酶复合物,负责选择性降解泛素化蛋白,这对于维持细胞内稳态和调节各种细胞过程至关重要。26S蛋白酶体由20S核心颗粒(具有蛋白水解活性)和两个19S调节颗粒(促进底物的识别和解折叠)组成。20S核心由14个呈圆柱状排列的亚基组成,外环由七个α亚基组成,内环包含七个β亚基,其中PSMB11在蛋白酶体的催化活性中起着重要作用。PSMB11在CD8阳性T细胞中尤为重要,它通过降低类似胰蛋白酶的活性来帮助调节免疫反应,从而影响免疫信号传导中特定蛋白的降解。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PSMB11 抗体 (H-1) 参考文献:

    1. 萎缩, 肥大和低氧血症诱导大鼠心脏泛素蛋白酶体系统的转录调节因子。  |  Razeghi, P., et al. 2006. Biochem Biophys Res Commun. 342: 361-4. PMID: 16483544
    2. 小鼠体内 26S 蛋白酶体催化亚基在间接抗氧化剂二硫代硫酮作用下的组织特异性增加:异常蛋白降解活性增强。  |  Kwak, MK., et al. 2007. Life Sci. 80: 2411-20. PMID: 17521679
    3. 胸腺特异性蛋白酶体对 CD8+ T 细胞发育的调控  |  Murata, S., et al. 2007. Science. 316: 1349-53. PMID: 17540904
    4. 小鼠 20S 蛋白酶体形成过程中的中间体:对前体 beta 亚基组装的影响。  |  Nandi, D., et al. 1997. EMBO J. 16: 5363-75. PMID: 9311996
    5. 编码人类蛋白酶体 beta 亚基和蛋白酶体 PA28 复合物基因的遗传关系。  |  McCusker, D., et al. 1997. Genomics. 45: 362-7. PMID: 9344661
    6. 14C]-3,4-二氯异香豆素与垂体和脾脏多催化蛋白酶复合物(蛋白酶体)亚基的反应。  |  Orlowski, M., et al. 1997. Biochemistry. 36: 13946-53. PMID: 9374874

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PSMB11 抗体 (H-1)

    sc-515132
    200 µg/ml
    $316.00

    PSMB11 (H-1): m-IgG1 BP-HRP 套装

    sc-531800
    200 µg Ab; 20 µg BP
    $354.00

    PSMB11 抗体 (H-1) AC

    sc-515132 AC
    500 µg/ml, 25% agarose
    $416.00

    PSMB11 抗体 (H-1) HRP

    sc-515132 HRP
    200 µg/ml
    $316.00

    PSMB11 抗体 (H-1) FITC

    sc-515132 FITC
    200 µg/ml
    $330.00

    PSMB11 抗体 (H-1) PE

    sc-515132 PE
    200 µg/ml
    $343.00

    PSMB11 抗体 (H-1) Alexa Fluor® 488

    sc-515132 AF488
    200 µg/ml
    $357.00

    PSMB11 抗体 (H-1) Alexa Fluor® 546

    sc-515132 AF546
    200 µg/ml
    $357.00

    PSMB11 抗体 (H-1) Alexa Fluor® 594

    sc-515132 AF594
    200 µg/ml
    $357.00

    PSMB11 抗体 (H-1) Alexa Fluor® 647

    sc-515132 AF647
    200 µg/ml
    $357.00

    PSMB11 抗体 (H-1) Alexa Fluor® 680

    sc-515132 AF680
    200 µg/ml
    $357.00

    PSMB11 抗体 (H-1) Alexa Fluor® 790

    sc-515132 AF790
    200 µg/ml
    $357.00

    PSMB11 (H-1) 中和勝肽

    sc-515132 P
    100 µg/0.5 ml
    $68.00

    does this product work with human thymus tissue that is fixed in 4% formalin?

    Asked by: Trant
    This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
    Answered by: Technical Support
    Date published: 2020-02-26

    I used this ab for IF on cryo sections of thymus tissue. I blocked the slide with 5% donkey serum in 0.2%PBS-T and did primary overnight (100X), following secondary stain for 2 hours, but I couldn't confirm positive staining. Do you have any suggestions?

    Asked by: TranT
    Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
    Answered by: Technical Support
    Date published: 2020-02-26

    does this product work well with human cell immunofluorescence staining?

    Asked by: Tran
    This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
    Answered by: Technical Support
    Date published: 2019-09-09

    What application is the blocking peptide sc-515132 P appropriate for?

    Asked by: Professor Griffin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2025, m_8, d_27, h_5CST
    • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasquestionsanswers, tq_4
    • loc_zh_CN, sid_515132, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getContent, 115ms
    • QUESTIONS, PRODUCT
    Rated 5 out of 5 by from Great for Western Blot detection of PSMB11This antibody works consistently in human cell lines Jurkat and RT-4. Shown with Cruz Marker molecular weight standards, sc-2035. -SCBT QC
    Date published: 2023-09-14
    • y_2025, m_8, d_27, h_5
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_zh_CN, sid_515132, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getReviews, 19ms
    • REVIEWS, PRODUCT
    PSMB11 抗体 (H-1) is rated 5.0 out of 5 by 1.
    • y_2025, m_8, d_27, h_5
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_zh_CN, sid_515132, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getAggregateRating, 104ms
    • REVIEWS, PRODUCT