Date published: 2025-9-9

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PRAM-1 抗体 (D-11): sc-166267

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说明书
  • PRAM-1 抗体 D-11 是小鼠单克隆 IgG1 (kappa轻链),PRAM-1抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的PRAM-1的内部区域内的氨基酸321-348之间
  • PRAM-1 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PRAM-1
  • 抗PRAM-1抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PRAM-1 (D-11): sc-166267免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是PRAM-1 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PRAM-1 Antibody (D-11) 打包提供(请参阅下面的订购信息)。

    快捷链接

    関連項目

    PRAM-1抗体(D-11)是一种IgG1 κ小鼠单克隆PRAM-1抗体(也称为PRAM-1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PRAM-1蛋白。PRAM-1抗体(D-11)既可以作为非偶联的抗PRAM-1抗体形式提供,也可以作为多种偶联形式的抗PRAM-1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。通过用维甲酸治疗患者,可以实现急性早幼粒细胞白血病的完全缓解,而PML-RAR-α(早幼粒细胞白血病-维甲酸受体α融合蛋白)在介导维甲酸对白血病细胞的作用中起主要作用。维甲酸诱导基因PRAM-1(PML-RAR-α靶基因,编码衔接分子1)编码一种衔接蛋白,该蛋白在正常人体髓系生成过程中表达并受到调节。PRAM-1的表达受到PML-RAR-α表达的阻碍。由718个氨基酸组成的PRAM-1蛋白包含八个N端富含脯氨酸的重复序列和几个聚集为I型或II型SH3识别基序的脯氨酸残基。PRAM-1在造血组织和肺中表达。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PRAM-1 抗体 (D-11) 参考文献:

    1. 在急性早幼粒细胞白血病细胞中,PRAM-1是一种受视黄酸(RA)和早幼粒细胞白血病(PML)-RA受体α调控的新型适配蛋白。  |  Moog-Lutz, C., et al. 2001. J Biol Chem. 276: 22375-81. PMID: 11301322
    2. PRAM-1是依赖整合素的中性粒细胞发挥最佳功能的必要条件。  |  Clemens, RA., et al. 2004. Mol Cell Biol. 24: 10923-32. PMID: 15572693
    3. PRAM-1 可增强三氧化二砷诱导的 JNK 激活。  |  Denis, FM., et al. 2005. J Biol Chem. 280: 9043-8. PMID: 15637062
    4. 免疫细胞适配蛋白中的脂质结合 hSH3 结构域。  |  Heuer, K., et al. 2006. J Mol Biol. 361: 94-104. PMID: 16831444
    5. 对接受三氧化二砷一线治疗的新诊断急性早幼粒细胞白血病患者的 PML-RAR alpha 进行实时 PCR 分析。  |  Ghaffari, SH., et al. 2006. Ann Oncol. 17: 1553-9. PMID: 16831853
    6. 非蛋白水解激活脯氨肽对心血管的影响  |  Susic, D., et al. 2006. Hypertension. 48: e113; author reply e114. PMID: 17043161
    7. 急性早幼粒细胞白血病中的溶酶体和三价砷治疗。  |  Kitareewan, S., et al. 2007. J Natl Cancer Inst. 99: 41-52. PMID: 17202112
    8. 对巩固治疗后 PML-RARalpha 转录本阴性的急性早幼粒细胞白血病患者进行的一项随机研究:日本成人白血病研究小组(JALSG)APL97 研究。  |  Asou, N., et al. 2007. Blood. 110: 59-66. PMID: 17374742

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PRAM-1 抗体 (D-11)

    sc-166267
    200 µg/ml
    $316.00

    PRAM-1 (D-11): m-IgG Fc BP-HRP 套装

    sc-528935
    200 µg Ab; 10 µg BP
    $354.00

    PRAM-1 (D-11): m-IgGκ BP-HRP 套装

    sc-521550
    200 µg Ab, 40 µg BP
    $354.00

    PRAM-1 (D-11): m-IgG1 BP-HRP 套装

    sc-543222
    200 µg Ab; 20 µg BP
    $354.00

    PRAM-1 抗体 (D-11) AC

    sc-166267 AC
    500 µg/ml, 25% agarose
    $416.00

    PRAM-1 抗体 (D-11) HRP

    sc-166267 HRP
    200 µg/ml
    $316.00

    PRAM-1 抗体 (D-11) FITC

    sc-166267 FITC
    200 µg/ml
    $330.00

    PRAM-1 抗体 (D-11) PE

    sc-166267 PE
    200 µg/ml
    $343.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 488

    sc-166267 AF488
    200 µg/ml
    $357.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 546

    sc-166267 AF546
    200 µg/ml
    $357.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 594

    sc-166267 AF594
    200 µg/ml
    $357.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 647

    sc-166267 AF647
    200 µg/ml
    $357.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 680

    sc-166267 AF680
    200 µg/ml
    $357.00

    PRAM-1 抗体 (D-11) Alexa Fluor® 790

    sc-166267 AF790
    200 µg/ml
    $357.00

    PRAM-1 (D-11) 中和勝肽

    sc-166267 P
    100 µg/0.5 ml
    $68.00

    What application is the blocking peptide sc-166267 P appropriate for?

    Asked by: Randy McDonald
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2025, m_8, d_27, h_5CST
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    Rated 5 out of 5 by from GOOD ANTIBODYThe results of immunofluorescence are good, but WB results need to be done again.
    Date published: 2017-10-18
    Rated 4 out of 5 by from GREAT FEATUREThere no bands in NB4 cell after treat with ATRA ,We are try best to find a solution,if there is better advise ,please contact me at any time.I hope to get much more information about this antibody in experiment of WB. THANK YOU
    Date published: 2017-05-28
    Rated 5 out of 5 by from Good Western blot data of PRAMGood Western blot data of PRAM-1 expression in non-transfected and human PRAM-1 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-01-07
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    PRAM-1 抗体 (D-11) is rated 4.7 out of 5 by 3.
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