PNP Antibody (D-8): sc-365132
- PNP Antibody (D-8) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 95-150 mapping within an internal region of PNP of human origin
- recommended for detection of PNP of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PNP Antibody (D-8) for WB applications. These reagents are now offered in bundles with PNP Antibody (D-8) (see ordering information below).
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PNP Antibody (D-8) is a mouse monoclonal IgG2a antibody that detects purine nucleoside phosphorylase (PNP) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PNP antibody (D-8) recognizes PNP, a crucial enzyme that catalyzes the reversible phosphorolysis of ribonucleosides and 2′-deoxyribonucleosides, specifically targeting guanine and hypoxanthine. This enzymatic activity is vital for purine metabolism, as PNP helps regulate nucleoside levels in the cell, thereby influencing nucleotide synthesis and degradation pathways. Proper functioning of PNP is essential for maintaining immune system health, as deficiencies in this enzyme can lead to severe immunological disorders, including combined immunodeficiency, which is particularly detrimental in children. PNP deficiency results in impaired T-lymphocyte function, making affected individuals highly susceptible to infections, autoimmune diseases, neurological impairments, and even cancer. PNP (D-8) antibody serves as an invaluable tool for researchers studying purine metabolism and its implications in various diseases, providing insights into PNP′s role in cellular function and disease pathology.
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PNP Antibody (D-8) References:
- Crystal structure of human PNP complexed with hypoxanthine and sulfate ion. | Canduri, F., et al. 2005. Biochem Biophys Res Commun. 326: 335-8. PMID: 15582582
- The transition state analog inhibitor of Purine Nucleoside Phosphorylase (PNP) Immucillin-H arrests bone loss in rat periodontal disease models. | Deves, C., et al. 2013. Bone. 52: 167-75. PMID: 23026564
- Evidence for purine nucleoside phosphorylase (PNP) release from rat C6 glioma cells. | Giuliani, P., et al. 2017. J Neurochem. 141: 208-221. PMID: 28251649
- 4-Nitrophenol (PNP) inhibits the expression of estrogen receptor β and disrupts steroidogenesis during the ovarian development in female rats. | Zhang, H., et al. 2017. Environ Pollut. 229: 1-9. PMID: 28570923
- PNP inhibitors selectively kill cancer cells lacking SAMHD1. | Davenne, T. and Rehwinkel, J. 2020. Mol Cell Oncol. 7: 1804308. PMID: 33235905
- Intratumoral immunotherapy using platelet-cloaked nanoparticles enhances antitumor immunity in solid tumors. | Bahmani, B., et al. 2021. Nat Commun. 12: 1999. PMID: 33790276
- PNP Hydrogel Prevents Formation of Symblephara in Mice After Ocular Alkali Injury. | Swarup, A., et al. 2022. Transl Vis Sci Technol. 11: 31. PMID: 35191963
- Platelet membrane-coated alterbrassicene A nanoparticle inhibits calcification of the aortic valve by suppressing phosphorylation P65 NF-κB. | Geng, B., et al. 2023. Theranostics. 13: 3781-3793. PMID: 37441596
- ADA and PNP deficiencies: how it all began. | Giblett, ER. 1985. Ann N Y Acad Sci. 451: 1-8. PMID: 3935025
- Localization of phosphoneuroprotein 14 (PNP 14) and its mRNA expression in rat brain determined by immunocytochemistry and in situ hybridization. | Nakajo, S., et al. 1994. Brain Res Mol Brain Res. 27: 81-6. PMID: 7877458
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PNP Antibody (D-8) | sc-365132 | 200 µg/ml | $322.00 | |||
PNP Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-535157 | 200 µg Ab; 40 µg BP | $361.00 | |||
PNP Antibody (D-8): m-IgG2a BP-HRP Bundle | sc-546214 | 200 µg Ab; 10 µg BP | $361.00 |