Date published: 2025-9-6

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PLEKHA4 抗体 (H-1): sc-376408

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说明书
  • PLEKHA4 抗体 H-1 是小鼠单克隆 IgG1 (kappa轻链),PLEKHA4抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的PLEKHA4的内部区域内的氨基酸427-459之间
  • PLEKHA4 抗体 (H-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PLEKHA4
  • 抗PLEKHA4抗体(H-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PLEKHA4 (H-1): sc-376408免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 PLEKHA4 抗体 (H-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与PLEKHA4 抗体 (H-1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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    関連項目

    PLEKHA4抗体(H-1)是一种IgG1 κ小鼠单克隆PLEKHA4抗体(也称为PLEKHA4抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的PLEKHA4蛋白。PLEKHA4抗体(H-1)既可作为非偶联形式的抗PLEKHA4抗体提供,也可作为多种偶联形式的抗PLEKHA4抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。PLEKHA4(含Pleckstrin同源结构域的家族A成员4),也称为PEPP1(磷酸肌醇3-磷酸结合蛋白1),是一种含779个氨基酸的蛋白质,含有一个Pleckstrin同源(PH)结构域,该结构域存在于参与细胞内信号传导的蛋白质中。PLEKH4A特异性地与早期内体上的磷脂酰肌醇-3-磷酸(PtdIns3P)结合,但不与其他磷酸肌醇结合。虽然在正常骨骼肌、小肠、肝、心和肾中的检测水平较低,但发现PLEKHA4在黑色素瘤中高表达。编码PLEKH4的基因定位于人类19号染色体,该染色体由超过6300万个碱基组成,包含约1400个基因,并被认为是人类染色体中基因密度最高的染色体。PLEKHA4有两种异构体,是选择性剪接事件的结果。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PLEKHA4 抗体 (H-1) 参考文献:

    1. 疾病基因与染色体:人类基因组疾病图谱。19 号染色体  |  Gilbert, F. 1997. Genet Test. 1: 145-9. PMID: 10464639
    2. 鉴定具有新型磷脂结合特异性的含褶曲蛋白组蛋白  |  Dowler, S., et al. 2000. Biochem J. 351: 19-31. PMID: 11001876
    3. 褶皱同源结构域中允许特异性识别磷酸肌醇的分子决定因素。  |  Lemmon, MA. and Ferguson, KM. 2001. Biochem Soc Trans. 29: 377-84. PMID: 11497993
    4. pleckstrin同源结构域的膜靶向作用。  |  Cozier, GE., et al. 2004. Curr Top Microbiol Immunol. 282: 49-88. PMID: 14594214
    5. 人类 19 号染色体的 DNA 序列和生物学特性。  |  Grimwood, J., et al. 2004. Nature. 428: 529-35. PMID: 15057824
    6. Pleckstrin 同源结构域:不仅适用于磷脂酰肌醇。  |  Lemmon, MA. 2004. Biochem Soc Trans. 32: 707-11. PMID: 15493994
    7. 透膜笼 PtdIns(3)P 的活化可诱导细胞内体融合。  |  Subramanian, D., et al. 2010. Nat Chem Biol. 6: 324-6. PMID: 20364126

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PLEKHA4 抗体 (H-1)

    sc-376408
    200 µg/ml
    $316.00

    PLEKHA4 (H-1): m-IgGκ BP-HRP 套装

    sc-522947
    200 µg Ab, 40 µg BP
    $354.00

    PLEKHA4 抗体 (H-1) AC

    sc-376408 AC
    500 µg/ml, 25% agarose
    $416.00

    PLEKHA4 抗体 (H-1) HRP

    sc-376408 HRP
    200 µg/ml
    $316.00

    PLEKHA4 抗体 (H-1) FITC

    sc-376408 FITC
    200 µg/ml
    $330.00

    PLEKHA4 抗体 (H-1) PE

    sc-376408 PE
    200 µg/ml
    $343.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 488

    sc-376408 AF488
    200 µg/ml
    $357.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 546

    sc-376408 AF546
    200 µg/ml
    $357.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 594

    sc-376408 AF594
    200 µg/ml
    $357.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 647

    sc-376408 AF647
    200 µg/ml
    $357.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 680

    sc-376408 AF680
    200 µg/ml
    $357.00

    PLEKHA4 抗体 (H-1) Alexa Fluor® 790

    sc-376408 AF790
    200 µg/ml
    $357.00

    PLEKHA4 (H-1) 中和勝肽

    sc-376408 P
    100 µg/0.5 ml
    $68.00

    Hi! I have ordered a sample of the antibody but in Immunofluorescence I got the data. However in western blot it is not working in both overexpressed and endogenous samples. Can you please tell me if any specific condition is needed for preparation of the

    Asked by: Nilu1497
    Thank you for your question. Please contact our technical service team directly for troubleshooting advice.
    Answered by: Tech Support Europe
    Date published: 2023-08-15

    What application is the blocking peptide sc-376408 P appropriate for?

    Asked by: TinTin
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
    Date published: 2015-04-04
    Rated 5 out of 5 by from Produced positive Western Blot data of PLEKHA4Produced positive Western Blot data of PLEKHA4 expression in non-transfected and human PLEKHA4 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-10-26
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    PLEKHA4 抗体 (H-1) is rated 5.0 out of 5 by 2.
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