Date published: 2026-10-16

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PKC gamma 抗体 (D-4): sc-166424

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说明书
  • PKC gamma抗体(D-4)是小鼠单克隆IgG2a κ, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PKC γ的N-terminus的氨基酸7-33之间
  • 推荐用于 mouse, rat 和 human 来源的PKC γ WB, IP, IF 和 ELISA检测
  • 看 PKC gamma (C-4): sc-166385,可查看有标记的 PKC gamma 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是PKC gamma Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与PKC gamma Antibody (D-4) 打包提供(请参阅下面的订购信息)。
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蛋白激酶C(PKC)家族成员在多种细胞功能中发挥着关键的调节作用,包括细胞生长和分化、基因表达、激素分泌和膜功能。蛋白激酶C最初被鉴定为丝氨酸/苏氨酸蛋白激酶,其活性依赖于钙和磷脂。二酰甘油(DAG)和促肿瘤佛波酯与蛋白激酶C结合并激活它。蛋白激酶C可分为至少两大类,包括常规(c)蛋白激酶C亚型(α、βI、βII和γ)和新型(n)蛋白激酶C亚型(δ、ε、ζ、η、θ、λ/ι、µ和ν)。每种蛋白激酶C亚型在不同组织中的表达模式不同,蛋白激酶C家族成员在辅因子依赖性方面存在明显差异。例如,蛋白激酶C δ和ε的激酶活性不依赖于Ca2+。另一方面,大多数其他蛋白激酶C成员具有佛波酯结合活性和激酶活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC gamma 抗体 (D-4) 参考文献:

  1. 多巴胺能通过增加PKC-gamma表达和降低PKA活性抑制促胰液素刺激的胆汁分泌。  |  Glaser, S., et al. 2003. Am J Physiol Gastrointest Liver Physiol. 284: G683-94. PMID: 12505882
  2. LEDGF激活PKC伽马和晶状体上皮细胞间隙连接的拆卸。  |  Nguyen, TA., et al. 2003. Exp Eye Res. 76: 565-72. PMID: 12697420
  3. PKC gamma-Cre小鼠系的产生和表征。  |  Ding, YQ., et al. 2005. Genesis. 43: 28-33. PMID: 16106361
  4. 听力受损的C57BL/6J和BALB/c小鼠耳蜗核中PKC伽马表达与年龄相关增加。  |  Kou, ZZ., et al. 2011. J Chem Neuroanat. 41: 20-4. PMID: 21056652
  5. 挥发性麻醉剂的催眠作用由PKC-γ动力学介导。  |  Takeda, A., et al. 2013. Acta Neurochir Suppl. 118: 307-10. PMID: 23564155
  6. 链脲佐菌素诱导的糖尿病小鼠三叉神经脊核中PKCγ表达的增加与口面热痛觉过敏有关。  |  Xie, HY., et al. 2015. J Chem Neuroanat. 63: 13-9. PMID: 25561408
  7. PKC伽马突变小鼠海马长期增强的修饰。  |  Abeliovich, A., et al. 1993. Cell. 75: 1253-62. PMID: 8269509
  8. PKC伽马突变小鼠在空间学习和情境学习方面表现出轻微缺陷。  |  Abeliovich, A., et al. 1993. Cell. 75: 1263-71. PMID: 8269510
  9. 蛋白激酶C(PKC)家族成员的功能分化。PKC gamma与PKC alpha和-beta II以及nPKC epsilon的不同之处在于,它能够通过TPA反应元件介导-12-O-十四烷酰基-13-乙酰基-12-羟基-23-磷酰胆碱(TPA)反应的转录激活。  |  Hata, A., et al. 1993. J Biol Chem. 268: 9122-9. PMID: 8473351
  10. 蛋白激酶(PKC gamma)阳性神经元中Fos免疫反应的共同表达:对大脑学习区域的定量分析。  |  Ambalavanar, R., et al. 1993. Brain Res. 606: 315-8. PMID: 8490724

订购信息

产品名称产品编号规格价格数量收藏夹

PKC gamma 抗体 (D-4)

sc-166424
200 µg/ml
$322.00

PKC gamma (D-4): m-IgG Fc BP-HRP 套装

sc-540100
200 µg Ab; 10 µg BP
$361.00

PKC gamma (D-4): m-IgG2a BP-HRP 套装

sc-546850
200 µg Ab; 10 µg BP
$361.00

PKC gamma (D-4) 中和勝肽

sc-166424 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-166424 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by zjw_8 from great success by WBI have used this antibody for PKC γ detection with great success by WB, the result is very fine band at target position 80kda.I adviced use 1:400 to WB analysis.
Date published: 2017-08-19
Rated 5 out of 5 by huayuyikang from got the expected bandI use this antibdoy detect PKC γ protein in mouse brain tissue extracts.At begining I didn't know whether this protein is expressed in the sample.I highly recommend use the positive control to confrim the activity of the antbody. I use 1:600 dilution of the antibody,got the target band.
Date published: 2017-07-15
Rated 5 out of 5 by TS from Srong band with a little background in humanSrong band with a little background in human PKC transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-05-25
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PKC gamma 抗体 (D-4) is rated 5.0 out of 5 by 3.
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