Date published: 2026-10-16

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PKC 抗体 (MC5): sc-80

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说明书
  • PKC抗体(MC5)是小鼠单克隆IgG2a κ, 在103篇文献中引用,规格为200 µg/ml
  • bovine起源的PKC铰链区域(残基292-317)内的表位图谱
  • 抗-PKC 抗体 (MC5) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 PKC α, PKC β and PKC γ; 也和以下物种反应,包括: bovine
  • 抗PKC抗体(MC5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是PKC Antibody (MC5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与PKC Antibody (MC5) 打包提供(请参阅下面的订购信息)。
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関連項目

PKC抗体(MC5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠和人类样本中的PKC。PKC (MC5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。蛋白激酶C(PKC)家族对于调节细胞生长、分化、基因表达、激素分泌和膜动力学等广泛细胞功能至关重要。PKC蛋白主要位于细胞质中,激活后转移到细胞膜,这对于其在信号转导通路中的作用至关重要。这种转移对于下游信号级联的激活至关重要,从而影响各种生理过程。PKC同工型受二酰基甘油和肿瘤促进剂苯氧乙醇酯激活,可分为传统同工型和新型同工型,每种同工型具有不同的调节机制和组织特异性表达模式。了解PKC的定位和激活对于阐明其在健康和疾病中的作用至关重要,特别是在信号通路被破坏的癌症和其他病理中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PKC 抗体 (MC5) 参考文献:

  1. PKC在发育性甲状腺功能减退大鼠脑中的作用。  |  Zhang, HM. and Su, Q. 2014. Neurol Sci. 35: 1161-6. PMID: 24682728
  2. 多酚化合物和 PKC 信号传导。  |  Das, J., et al. 2016. Biochim Biophys Acta. 1860: 2107-21. PMID: 27369735
  3. 癌症、肿瘤促进和肿瘤抑制中的蛋白激酶C(PKC)异构体。  |  Isakov, N. 2018. Semin Cancer Biol. 48: 36-52. PMID: 28571764
  4. 心脏病中的 PKC 和 PKN  |  Marrocco, V., et al. 2019. J Mol Cell Cardiol. 128: 212-226. PMID: 30742812
  5. TRPM2通过PKC/MAPK途径促进胰腺癌的发生。  |  Lin, R., et al. 2021. Cell Death Dis. 12: 585. PMID: 34099637
  6. PKC-δ和PKC-ε在心肌缺血/再灌注损伤中的作用。  |  Chen, L., et al. 2021. Pharmacol Res. 170: 105716. PMID: 34102229
  7. 蛋白激酶C(PKC)的激活剂和抑制剂:其在临床试验中的应用。  |  Kawano, T., et al. 2021. Pharmaceutics. 13: PMID: 34834162
  8. 以小胶质细胞中的 PKC 为靶点促进中枢神经系统的髓鞘再形成和修复  |  Kim, PM. and Kornberg, MD. 2022. Curr Opin Pharmacol. 62: 103-108. PMID: 34965482
  9. PKC-β通过Stim1磷酸化调节Ca2+动员。  |  Song, HJ., et al. 2022. Genes Genomics. 44: 571-582. PMID: 35254656
  10. 靶向PKC可减轻糖尿病和血色素沉着症中的铁超负荷。  |  Banerjee, S., et al. 2023. bioRxiv.. PMID: 38076948

订购信息

产品名称产品编号规格价格数量收藏夹

PKC 抗体 (MC5)

sc-80
200 µg/ml
$322.00

PKC (MC5): m-IgG Fc BP-HRP 套装

sc-528095
200 µg Ab; 10 µg BP
$361.00

PKC (MC5): m-IgGκ BP-HRP 套装

sc-520393
200 µg Ab, 40 µg BP
$361.00

PKC (MC5): m-IgG2a BP-HRP 套装

sc-547023
200 µg Ab; 10 µg BP
$361.00

PKC 抗体 (MC5) AC

sc-80 AC
500 µg/ml, 25% agarose
$424.00

PKC 抗体 (MC5) HRP

sc-80 HRP
200 µg/ml
$322.00

PKC 抗体 (MC5) FITC

sc-80 FITC
200 µg/ml
$336.00

PKC 抗体 (MC5) PE

sc-80 PE
200 µg/ml
$349.00

PKC 抗体 (MC5) Alexa Fluor® 488

sc-80 AF488
200 µg/ml
$364.00

PKC 抗体 (MC5) Alexa Fluor® 546

sc-80 AF546
200 µg/ml
$364.00

PKC 抗体 (MC5) Alexa Fluor® 594

sc-80 AF594
200 µg/ml
$364.00

PKC 抗体 (MC5) Alexa Fluor® 647

sc-80 AF647
200 µg/ml
$364.00

PKC 抗体 (MC5) Alexa Fluor® 680

sc-80 AF680
200 µg/ml
$364.00

PKC 抗体 (MC5) Alexa Fluor® 790

sc-80 AF790
200 µg/ml
$364.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-02-02
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Rated 5 out of 5 by gkyriakopoulos from Excellent detectionI took great signals from all the lysates from lung adenocarcinoma cell lines
Date published: 2021-03-17
Rated 4 out of 5 by CristinaQG from Está bienEste anticuerpo recién comprado está bien pero da un poco de señal-ruido de fondo en inmunohistoquímica.
Date published: 2017-05-11
Rated 5 out of 5 by snowy from highly specificEyeballs were fixed in 4% Paraformaldehyde for 2h. Immunoreactivity in the mole-rat retina was observed exclusively in bipolar cells with this highly specific ab.
Date published: 2017-02-08
Rated 5 out of 5 by Like hao from Good WB data with rat skeletal muscleRecently,I used this monoclonal antibody 1:1000 with rat skeletal muscle,obtained the good bands which is my desired。
Date published: 2017-01-13
Rated 5 out of 5 by Randy McDonald from Very specific antibody fo western blotWe use this antibody for western blot and we got a very specific clear band around the correct molecular weight
Date published: 2017-01-11
Rated 5 out of 5 by LM from Produced a clear band with human recombinant PKCProduced a clear band with human recombinant PKC . -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by CJ from Works well for WB with NIH/3T3 and JurkatWorks well for WB with NIH/3T3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by NZ from Great staining with paraffinGreat staining with paraffin-embedded human gall bladder marrow. -SCBT QC
Date published: 2015-01-26
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PKC 抗体 (MC5) is rated 4.9 out of 5 by 9.
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