PKA alpha cat Antibody (H-8): sc-515039
- PKAα cat Antibody (H-8) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 31-48 near the N-terminus of PKAα cat of human origin
- recommended for detection of PKAα cat of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See PKAα cat (A-2): sc-28315 for PKAα cat antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PKAα cat Antibody (H-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PKAα cat Antibody (H-8) (see ordering information below).
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PKAα cat Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody specifically designed to recognize human Protein Kinase A catalytic subunit alpha (PKAα cat), also known as PRKACA or the Cα subunit. PKAα cat is a vital enzyme in the cyclic AMP (cAMP)-dependent signaling pathway, regulating diverse cellular processes such as metabolism, gene transcription, ion transport, and cell proliferation. Structurally, PKAα cat consists of an N-terminal domain, a catalytic domain, and a C-terminal domain, which are essential for kinase activity and interaction with regulatory subunits. Anti-PKAα cat antibody (H-8) recognizes an epitope mapped to amino acids 31-48 near the N-terminus, a region crucial for substrate recognition and binding. Post-translational modifications, including phosphorylation, play a significant role in modulating PKAα cat′s activity and stability, thereby influencing PKAα cat′s ability to phosphorylate target proteins and propagate cellular signals. Upon binding of cAMP, regulatory subunits dissociate from catalytic subunits, activating PKAα cat to phosphorylate specific serine and threonine residues on substrates such as the cAMP response element-binding protein (CREB), which is pivotal for initiating gene transcription. PKAα cat interacts with various proteins, including CREB and transcription factors, facilitating regulation of gene expression and maintaining cellular homeostasis. PKAα cat monoclonal antibody (H-8) is recommended for use in multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), and is effective across species such as mouse, rat, and human. PKAα cat (H-8) antibody is an indispensable tool for researchers investigating intricate mechanisms of cAMP signaling and its impact on cellular physiology, as well as for developing therapeutic strategies targeting dysregulated PKA activity in various diseases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PKAα cat Antibody (H-8) | sc-515039 | 200 µg/ml | $322.00 | |||
PKAα cat Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-538738 | 200 µg Ab; 10 µg BP | $361.00 | |||
PKAα cat Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-536403 | 200 µg Ab; 40 µg BP | $361.00 | |||
PKAα cat Antibody (H-8): m-IgG2a BP-HRP Bundle | sc-548388 | 200 µg Ab; 10 µg BP | $361.00 | |||
PKAα cat (H-8) Neutralizing Peptide | sc-515039 P | 100 µg/0.5 ml | $69.00 |