pirin Antibody (D-12): sc-271622
- pirin Antibody (D-12) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-290 representing full length pirin of human origin
- pirin Antibody (D-12) is recommended for detection of pirin of human origin by WB, IP, IF and ELISA
- Anti-pirin Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271622 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for pirin Antibody (D-12) for WB applications. These reagents are now offered in bundles with pirin Antibody (D-12) (see ordering information below).
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Pirin Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects human pirin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-pirin antibody (D-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pirin, also known as iron-binding nuclear protein, plays a crucial role in transcriptional activation and apoptosis, making pirin essential for cellular regulation and response to stress. Notably, pirin is localized within the nucleus, where pirin interacts with specific transcription factors, including NFI/CTF1 (nuclear factor I/CCAAT box transcription factor), suggesting pirin′s involvement as a cofactor in transcriptional regulation. This interaction provides insights into the mechanisms of gene expression and the regulation of DNA transcription and replication, highlighting pirin′s importance in maintaining cellular homeostasis and responding to various physiological stimuli. Research indicates that pirin mRNA is poorly expressed across human tissues, with notable expression in heart and skeletal muscle, suggesting a specialized role in these tissues. The unique properties and interactions of pirin make pirin monoclonal antibody (D-12) a valuable tool for researchers studying transcriptional regulation and related cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
pirin Antibody (D-12) References:
- A tomato homologue of the human protein PIRIN is induced during programmed cell death. | Orzaez, D., et al. 2001. Plant Mol Biol. 46: 459-68. PMID: 11485202
- Purification, crystallization and preliminary X-ray analysis of human pirin. | Zeng, Q., et al. 2003. Acta Crystallogr D Biol Crystallogr. 59: 1496-8. PMID: 12876364
- Crystal structure of human pirin: an iron-binding nuclear protein and transcription cofactor. | Pang, H., et al. 2004. J Biol Chem. 279: 1491-8. PMID: 14573596
- A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions. | Hihara, Y., et al. 2004. FEBS Lett. 574: 101-5. PMID: 15358547
- Gene expression in response to anti-tumour intervention by polysaccharide-K (PSK) in colorectal carcinoma cells. | Yoshikawa, R., et al. 2004. Oncol Rep. 12: 1287-93. PMID: 15547752
- Structural and biochemical analysis reveal pirins to possess quercetinase activity. | Adams, M. and Jia, Z. 2005. J Biol Chem. 280: 28675-82. PMID: 15951572
- Upregulation of pirin expression by chronic cigarette smoking is associated with bronchial epithelial cell apoptosis. | Gelbman, BD., et al. 2007. Respir Res. 8: 10. PMID: 17288615
- Identification of pirin, a novel highly conserved nuclear protein. | Wendler, WM., et al. 1997. J Biol Chem. 272: 8482-9. PMID: 9079676
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pirin Antibody (D-12) | sc-271622 | 200 µg/ml | $322.00 | |||
pirin Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-525817 | 200 µg Ab; 10 µg BP | $361.00 | |||
pirin Antibody (D-12): m-IgG2a BP-HRP Bundle | sc-546427 | 200 µg Ab; 10 µg BP | $361.00 | |||
pirin Antibody (D-12) X | sc-271622 X | 200 µg/0.1 ml | $322.00 | |||
pirin Antibody (D-12) AC | sc-271622 AC | 500 µg/ml, 25% agarose | $424.00 | |||
pirin Antibody (D-12) HRP | sc-271622 HRP | 200 µg/ml | $322.00 | |||
pirin Antibody (D-12) FITC | sc-271622 FITC | 200 µg/ml | $336.00 | |||
pirin Antibody (D-12) PE | sc-271622 PE | 200 µg/ml | $349.00 | |||
pirin Antibody (D-12) Alexa Fluor® 488 | sc-271622 AF488 | 200 µg/ml | $364.00 | |||
pirin Antibody (D-12) Alexa Fluor® 546 | sc-271622 AF546 | 200 µg/ml | $364.00 | |||
pirin Antibody (D-12) Alexa Fluor® 594 | sc-271622 AF594 | 200 µg/ml | $364.00 | |||
pirin Antibody (D-12) Alexa Fluor® 647 | sc-271622 AF647 | 200 µg/ml | $364.00 | |||
pirin Antibody (D-12) Alexa Fluor® 680 | sc-271622 AF680 | 200 µg/ml | $364.00 | |||
pirin Antibody (D-12) Alexa Fluor® 790 | sc-271622 AF790 | 200 µg/ml | $364.00 |