Phosphate Buffered Saline: powder for 100 ml of 10X Ready to use for the preparation of 10X PBS; PBS solution is commonly used in biological research.

Phosphate Buffered Saline: powder for 100 ml of 10X

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同义词: 10X PBS (powder)
应用; Ready to use for the preparation of 10X PBS; PBS solution is commonly used in biological research
* Refer to Certificate of Analysis for lot specific data (including water content).

Phosphate Buffered Saline: powder for 100 ml of 10X is ready to use for preparation of 100 ml of concentrated 10X Phosphate-buffered saline (PBS) or 1L of 1X Phosphate-buffered saline (PBS). PBS buffer is ideal for maintaining a constant pH, and since it is isotonic and non-toxic to cells, it can be used in a wide variety of biological applications. PBS buffer is frequently used as a wash and dilution buffer, and is also used to take a reference spectrum when measuring protein adsorption.
Additional sizes, formats, and concentrations available:
Phosphate Buffered Saline: 1 L of 1X (sc-362182)
Phosphate Buffered Saline: 100 ml of 10X (sc-362297)
Phosphate Buffered Saline: 500 ml of 10X (sc-362298)
Phosphate Buffered Saline: 1 L of 10X (sc-24946)
Phosphate Buffered Saline: 1 L of 20X (sc-362183)
Phosphate Buffered Saline: 5 L of 20X (sc-362299)
Phosphate Buffered Saline: powder for 500 ml of 10X (sc-362301)
Phosphate Buffered Saline: powder for 1 L of 10X (sc-362302)
Phosphate Buffered Saline: powder for 5 L of 10X (sc-24947)


参考文献

1. Dulbecco, R. and Vogt, M. 1954. J. Exp. Med. 99: 167-182. PMID: 13130792
2. Wilcox, C.S., et al. 1992. Proc. Natl. Acad. Sci. U.S.A. 89: 11993-11997. PMID: 1281548
3. Fernando, P.N., et al. 2001. J Chromatogr A. 920: 155-162. PMID: 11452994
4. Kang, J., et al. 2009. Anal. Biochem. 386: 251-255. PMID: 19150324
5. Andersson, J. and Dahlgren, U. 2010. J Dent Res. PMID: 20811070
6. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor.Vol. 3.

用法 :
• Reconstitute powder in ddH20, bringing final volume to 100 ml (10X). Prior to use, dilute to a 1X solution and filter through a 0.22 µm filter into a sterile flask. Makes 1 L of 1X PBS buffer with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. The PBS solution pH will be approximately 7.4.
• If buffer is used in cell culture applications, sterilization of the solution may be necessary. Pre-diluted buffer can be dispensed into aliquots and sterilized by autoclaving (20 min, 121°C, liquid cycle).
• PBS can be stored at room temperature, but non-sterile solutions may warrant refrigeration to prevent bacterial growth over time. Concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
保存 :
Store at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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