Date published: 2025-9-10

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CD31/PECAM-1 抗体 (E-4): sc-365804

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说明书
  • CD31/PECAM-1抗体(E-4)是小鼠单克隆IgG1 κ, 在11篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PECAM-1的C-terminus的氨基酸712-738之间
  • 抗-CD31/PECAM-1 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 PECAM-1
  • 抗CD31/PECAM-1抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD31/PECAM-1 (E-4): sc-365804免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CD31/PECAM-1 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD31/PECAM-1 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD31/PECAM-1抗体(E-4)是一种IgG1 κ小鼠单克隆PECAM-1抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的PECAM-1。CD31/PECAM-1抗体(E-4)可作为非偶联抗PECAM-1抗体形式,也可作为多种偶联形式的抗PECAM-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞粘附分子是一组密切相关的细胞表面糖蛋白家族,参与生长过程中的细胞间相互作用,并被认为在胚胎发生和发育中发挥重要作用。神经元细胞粘附分子(NCAM)的表达在多种人类肿瘤中观察到,包括神经母细胞瘤、横纹肌肉瘤、Wilms肿瘤、Ewing肉瘤和一些原始髓系恶性肿瘤。细胞内粘附分子-1(ICAM-1),也称为CD54,是免疫球蛋白超家族的一个整合膜蛋白,可识别β2/α1和β2/αM整合素。PECAM-1(血小板/内皮细胞粘附分子-1),也称为CD31,是表达于单核细胞、中性粒细胞、血小板和T细胞亚群的细胞表面的糖蛋白。VCAM-1(血管细胞粘附分子-1)最初被鉴定为由炎症细胞因子(如IL-1、肿瘤坏死因子(TNF)和脂多糖(LPS))在人内皮细胞上诱导的粘附分子。KALIG基因编码一种类似神经细胞粘附分子(NCAM)的蛋白质,在66%的Kallmann综合征患者中缺失,该综合征患者患有继发性性腺功能减退症的嗅觉缺失。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD31/PECAM-1 抗体 (E-4)

sc-365804
200 µg/ml
$316.00

CD31/PECAM-1 (E-4): m-IgG Fc BP-HRP 套装

sc-529474
200 µg Ab; 10 µg BP
$354.00

CD31/PECAM-1 (E-4): m-IgGκ BP-HRP 套装

sc-522375
200 µg Ab, 40 µg BP
$354.00

CD31/PECAM-1 (E-4): m-IgG1 BP-HRP 套装

sc-543531
200 µg Ab; 20 µg BP
$354.00

CD31/PECAM-1 抗体 (E-4) AC

sc-365804 AC
500 µg/ml, 25% agarose
$416.00

CD31/PECAM-1 抗体 (E-4) HRP

sc-365804 HRP
200 µg/ml
$316.00

CD31/PECAM-1 抗体 (E-4) FITC

sc-365804 FITC
200 µg/ml
$330.00

CD31/PECAM-1 抗体 (E-4) PE

sc-365804 PE
200 µg/ml
$343.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 488

sc-365804 AF488
200 µg/ml
$357.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 546

sc-365804 AF546
200 µg/ml
$357.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 594

sc-365804 AF594
200 µg/ml
$357.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 647

sc-365804 AF647
200 µg/ml
$357.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 680

sc-365804 AF680
200 µg/ml
$357.00

CD31/PECAM-1 抗体 (E-4) Alexa Fluor® 790

sc-365804 AF790
200 µg/ml
$357.00

CD31/PECAM-1 (E-4) 中和勝肽

sc-365804 P
100 µg/0.5 ml
$68.00

Hi. I want to try IHC-P but I wonder which antigen retrieval you use and for how long. Do you have the protocols somewhere in your web? Thanks

Asked by: Machetero
Thank you for your question. We recommend using sodium citrate buffer plus heat treatment for antigen retrieval with this mouse monoclonal antibody, PECAM-1 (E-4): sc-365804. An excerpt from our protocol is provided below, as well as the link to the complete protocol. Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining A complete list of our protocols is available on our website listed at the very bottom of the page, below "Resources". A link is also provided below for your reference. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support
Date published: 2023-05-05

What application is the blocking peptide sc-365804 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from cd31The product is in high titer, satisfied with stain,and worth being intriduced
Date published: 2017-09-20
Rated 5 out of 5 by from WB: Positive data in Jurkat whole cell lysateWB: Positive data in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Worked great for Western in human PBLWorked great for Western in human PBL, THP-1 whole cell lysates and human platelet extract. -SCBT QC
Date published: 2013-10-13
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CD31/PECAM-1 抗体 (E-4) is rated 5.0 out of 5 by 4.
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