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Pdcd2L 抗体 (K-13): sc-101251

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说明书
  • Pdcd2L 抗体 K-13 是小鼠单克隆 IgG1 κ,Pdcd2L抗体, 在1篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组Pdcd2L
  • 推荐用于 mouse, rat 和 human 来源的Pdcd2L WB, IP, IF 和 ELISA检测
  • 关于如何获取Pdcd2L (K-13): sc-101251免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Pdcd2L 抗体 (K-13)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Pdcd2L抗体(K-13)是一种IgG1 κ小鼠单克隆Pdcd2L抗体(也称为Pdcd2L抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Pdcd2L蛋白。Pdcd2L抗体(K-13)为非偶联形式的抗Pdcd2L抗体。Pdcd-1(程序性细胞死亡-1蛋白)是一种I型跨膜受体,属于免疫球蛋白基因超家族成员。在小鼠胸腺中检测到Pdcd-1的表达,并在受刺激的B和T细胞系中被诱导表达,在这些细胞中,它可能在各种免疫反应的负调控中发挥作用。Pdcd-2(程序性细胞死亡-2),也称为PD-2、PDL2或B7DC,在胎盘、心脏、胰腺、肺和肝脏中高表达,在脾脏、淋巴结和胸腺中低表达。Pdcd2L(程序性细胞死亡2样),也称为MGC13096,是一种由358个氨基酸组成的蛋白质,与Pdcd-2相似,可能在细胞凋亡中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pdcd2L 抗体 (K-13) 参考文献:

  1. 参与 T 细胞凋亡的新型小鼠 cDNA ES18 的特征。  |  Park, EJ., et al. 1999. Nucleic Acids Res. 27: 1524-30. PMID: 10037816
  2. 暂时性局灶性脑缺血后大鼠大脑中与细胞凋亡相关的基因 2 (ALG2) 的表达增加。  |  Li, W., et al. 2000. Neuroscience. 96: 161-8. PMID: 10683420
  3. PD-L2 是 PD-1 的第二配体,可抑制 T 细胞的活化。  |  Latchman, Y., et al. 2001. Nat Immunol. 2: 261-8. PMID: 11224527
  4. 程序性细胞死亡诱导免疫球蛋白基因超家族新成员 PD-1 的表达。  |  Ishida, Y., et al. 1992. EMBO J. 11: 3887-95. PMID: 1396582
  5. 对伽马射线敏感的 SX9 细胞和对伽马射线耐受的 SR-1 细胞中 DNA 拷贝数以及细胞周期调控和细胞凋亡信号通路相关基因的表达变化。  |  Koshikawa, T., et al. 2005. Radiat Res. 163: 374-83. PMID: 15799692
  6. 甲磺酸伊马替尼联合三氧化二砷在慢性粒细胞白血病中协调内在, 外在和内质网介导的细胞凋亡。  |  Du, Y., et al. 2006. Blood. 107: 1582-90. PMID: 16249384
  7. 克隆具有 PDCD2(C)结构域的 cDNA 及其在 HEK293T 细胞凋亡过程中的表达。  |  Chen, Q., et al. 2005. Mol Cell Biochem. 280: 185-91. PMID: 16311922
  8. 非肥胖型糖尿病淋巴细胞的应激系统失调  |  Liu, Z. and Aune, TM. 2006. Genes Immun. 7: 352-8. PMID: 16691184
  9. 受刺激的小鼠 T 和 B 淋巴细胞表面的 PD-1 抗原表达。  |  Agata, Y., et al. 1996. Int Immunol. 8: 765-72. PMID: 8671665

订购信息

产品名称产品编号规格价格数量收藏夹

Pdcd2L 抗体 (K-13)

sc-101251
100 µg/ml
$333.00

Hi there, I am curious as to what the sequence of the antibody is for Pdcd2L Antibody (K-13): sc-101251. We are trying to use a peptide control. Thank you, Brendan

Asked by: Brendan1
Thank you for your question. This antibody, sc-101251, was raised against recombinant Pdcd2L of human origin spanning amino acids 80-179. Since it is not a peptide antibody, and the epitope location has not been mapped, you will not be able to do a peptide neutralization unless you epitope map the antibody. I would suggest using a normal mouse IgG for a negative control.
Answered by: Tech Service
Date published: 2019-09-16

I am using Pdcd2L (K-13): sc-101251 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good choice for Immunoperoxidase stainingAntibody has been used for Immunoperoxidase staining of human and mouse testis tissue in published research. -SCBT QC
Date published: 2023-09-14
Rated 3 out of 5 by from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-26
Rated 3 out of 5 by from Produced satisfactory immunofluorescenceProduced satisfactory immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-22
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Pdcd2L 抗体 (K-13) is rated 3.7 out of 5 by 3.
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