Date published: 2025-9-8

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PCB 抗体 (H-2): sc-271493

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说明书
  • PCB 抗体 H-2 是小鼠单克隆 IgG1 κ,PCB抗体, 在22篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的PCB的C-terminus附近的氨基酸954-985之间
  • PCB 抗体 (H-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PCB; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗PCB抗体(H-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PCB (H-2): sc-271493免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是PCB Antibody (H-2) for WB applications. 的首选辅助检测试剂,这些试剂现与PCB Antibody (H-2) 打包提供(请参阅下面的订购信息)。

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関連項目

PCB抗体(H-2)是一种IgG1 κ小鼠单克隆PCB抗体(也称为PC抗体或丙酮酸羧化酶抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的PCB蛋白。PCB抗体(H-2)既可以非偶联抗PCB抗体的形式提供,也可以多种偶联形式的抗PCB抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丙酮酸羧化酶(PCB)是一种生物素化的线粒体酶,它催化丙酮酸以组织特异性方式合成草酰乙酸。除了与生物素辅因子共价结合外,PCB还含有ATP和底物丙酮酸附着的共有序列。PCB基因位于11号染色体的长臂上。已知PCB代谢的突变(丙酮酸羧化酶缺乏症)会导致乳酸性酸中毒、低血糖和精神发育迟滞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PCB 抗体 (H-2) 参考文献:

  1. 遗传性肥胖中的丙酮酸羧化酶  |  Lynch, CJ., et al. 1992. Am J Physiol. 262: E608-18. PMID: 1375435
  2. 大脑中的细胞间代谢分区:过去, 现在和未来。  |  Hertz, L. 2004. Neurochem Int. 45: 285-96. PMID: 15145544
  3. INS-1细胞中无性繁殖途径的13C NMR同位素分析。  |  Cline, GW., et al. 2004. J Biol Chem. 279: 44370-5. PMID: 15304488
  4. 转羧酶 5S 结构:多酶复合物亚基的组装和催化机制。  |  Hall, PR., et al. 2004. EMBO J. 23: 3621-31. PMID: 15329673
  5. 高氨血症伴瓜氨酸血症。  |  Karnik, D., et al. 2004. Indian Pediatr. 41: 842-4. PMID: 15347874
  6. 碳酸酐酶抑制剂:用阴离子抑制膜结合的人类同工酶 IV。  |  Innocenti, A., et al. 2004. Bioorg Med Chem Lett. 14: 5769-73. PMID: 15501038
  7. 生物素缺乏会降低人体血液白细胞中潜在生物素转运体 SLC19A3 的表达。  |  Vlasova, TI., et al. 2005. J Nutr. 135: 42-7. PMID: 15623830
  8. 人丙酮酸羧化酶 cDNA 的分子克隆。与其他含生物素羧化酶的结构关系以及对分化前脂肪细胞中 mRNA 含量的调控。  |  Freytag, SO. and Collier, KJ. 1984. J Biol Chem. 259: 12831-7. PMID: 6548474
  9. 克隆人肾丙酮酸羧化酶的 cDNA。  |  MacKay, N., et al. 1994. Biochem Biophys Res Commun. 202: 1009-14. PMID: 8048912
  10. 多药耐药性 P-糖蛋白单克隆抗体 JSB-1 与丙酮酸羧化酶发生交叉反应。  |  Rao, VV., et al. 1995. J Histochem Cytochem. 43: 1187-92. PMID: 8537634
  11. 丙酮酸羧化酶和丙酮酸脱氢酶复合物失调。  |  Robinson, BH., et al. 1996. J Inherit Metab Dis. 19: 452-62. PMID: 8884569
  12. 两个近亲家庭丙酮酸羧化酶缺乏症的分子特征。  |  Wexler, ID., et al. 1998. Pediatr Res. 43: 579-84. PMID: 9585002

订购信息

产品名称产品编号规格价格数量收藏夹

PCB 抗体 (H-2)

sc-271493
200 µg/ml
$316.00

PCB (H-2): m-IgG Fc BP-HRP 套装

sc-527249
200 µg Ab; 10 µg BP
$354.00

PCB (H-2): m-IgG1 BP-HRP 套装

sc-532622
200 µg Ab; 20 µg BP
$354.00

PCB 抗体 (H-2) AC

sc-271493 AC
500 µg/ml, 25% agarose
$416.00

PCB 抗体 (H-2) HRP

sc-271493 HRP
200 µg/ml
$316.00

PCB 抗体 (H-2) FITC

sc-271493 FITC
200 µg/ml
$330.00

PCB 抗体 (H-2) PE

sc-271493 PE
200 µg/ml
$343.00

PCB 抗体 (H-2) Alexa Fluor® 488

sc-271493 AF488
200 µg/ml
$357.00

PCB 抗体 (H-2) Alexa Fluor® 546

sc-271493 AF546
200 µg/ml
$357.00

PCB 抗体 (H-2) Alexa Fluor® 594

sc-271493 AF594
200 µg/ml
$357.00

PCB 抗体 (H-2) Alexa Fluor® 647

sc-271493 AF647
200 µg/ml
$357.00

PCB 抗体 (H-2) Alexa Fluor® 680

sc-271493 AF680
200 µg/ml
$357.00

PCB 抗体 (H-2) Alexa Fluor® 790

sc-271493 AF790
200 µg/ml
$357.00

PCB (H-2) 中和勝肽

sc-271493 P
100 µg/0.5 ml
$68.00

I am very interested in Anti-PCB Antibody (H-2): sc-271493. Would you like to give us a sample to try?

Asked by: Mengjie
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What application is the blocking peptide sc-271493 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Two bandsI got two bands in my western. The molecular weight is so close that I can not tell which one is the PCB band.
Date published: 2018-04-17
Rated 5 out of 5 by from High Quality ProductThe PCB Antibody (H-2): sc-271493 works very well for immunofluorescence applications, showing strong staining pattern with cytoplasmic localization. This antibody has also been cited several times for further verification.
Date published: 2017-01-30
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Satisfactory Western Blot data of PCB expressionSatisfactory Western Blot data of PCB expression in 293T whole cell lysate. -SCBT QC
Date published: 2013-03-28
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PCB抗体 (H-2) is rated 4.8 out of 5 by 4.
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