PBEF抗体 (E-3) 推荐用于 mouse, rat 和 human 来源的PBEF WB, IP, IF 和 ELISA检测

PBEF 抗体 (E-3): sc-393444

PBEF抗体 (E-3) is rated 5.0 out of 5 by 13.
  • y_2021, m_6, d_13, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_393444, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 159ms
  • REVIEWS, PRODUCT
loading
5
13
4
0
3
0
2
0
1
0

说明书

    • 抗-PBEF 抗体 E-3 是小鼠单克隆 IgG2b κ,PBEF抗体, 在2篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列1-300位于human物种的PBEF的N-terminus
    • 抗-PBEF 抗体 (E-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 PBEF
    • 抗-PBEF 抗体 (E-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取PBEF (E-3): sc-393444免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 对于PBEF 抗体 (E-3)的WB应用,m-IgGκ BP-HRP 是首选的二抗检测试剂。现在该试剂和PBEF 抗体 (E-3)组合成套装提供(参见下面的订购信息)。对于其他m-IgGκ BP 偶联产品,参见我们小鼠IgG 结合蛋白的完整列表
loading

What is the recommended fixation method for immunofluorescence staining with PBEF (E-3): sc-393444 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
  • y_2021, m_6, d_13, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_393444, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 148ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very good antibody I used this antibody in mouse liver and muscle samples. This antibody works well in both tissues. I was able to get clean bands in WB with 1:500 dilution (by both fluorescence and chemiluminescence).
Date published: 2020-07-27
Rated 5 out of 5 by from Works well Probed at 1:500 overnight, detected strong bands at the right size
Date published: 2017-11-15
Rated 5 out of 5 by from Satisfied with the antibody PBEF antibody provides great staining in IF and IHC applications, binding is both strong and specific.The antibody could be performed under isothermal conditions, its specificity was good and the sensitivity.
Date published: 2017-10-09
Rated 5 out of 5 by from This antibody is very good. PBEF antibody works well for WB at the concentration of 1:200, with satisfied bands.
Date published: 2017-05-23
Rated 5 out of 5 by from Excellent for WB it works excellent for WB in our lab, will keep usinig it
Date published: 2017-05-08
Rated 5 out of 5 by from Excellent antibody! I apply for the sample sack of this antibody,the band is special and clear.
Date published: 2017-03-29
Rated 5 out of 5 by from The Product is Good We tried it detect this sample from dilution 1:800. The researchers confirmed that PBEF Antibody accurately detects rat with high sensitivity and specificity. We were convinced the antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from WB and worked fine This gave very strong signal in our Western, we use dilution to 1:400 and it worked well.
Date published: 2017-02-12
  • y_2021, m_6, d_13, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_393444, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话(021) 800-820-8626。 版权 ©, 2007-2021圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。