Date published: 2025-9-8
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Pax-6 Antibody (PAX6) - Western Blotting - Image 375143
Pax-6 抗体 (PAX6): sc-81649. AN3 CA (A) 和 ECV304 (B) 全细胞裂解液中 Pax-6 表达的 Western 印迹分析.
Pax-6 抗体 (PAX6): sc-81649. AN3 CA (A) 和 ECV304 (B) 全细胞裂解液中 Pax-6 表达的 Western 印迹分析.

Pax-6 抗体 (PAX6): sc-81649

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说明书
  • Pax-6 抗体 PAX6 是小鼠单克隆 IgG1 κ，Pax-6抗体, 在65篇文献中引用，规格为200 µg/ml
  • 免疫chicken物种的Pax-6的氨基酸1-223
  • Pax-6 抗体 (PAX6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和avian 来源的 Pax-6
  • 抗Pax-6抗体（PAX6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Pax-6 (PAX6): sc-81649的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Pax-6 抗体 (PAX6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Pax-6 抗体 (PAX6) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Pax 抗体包括 Pax-2, Pax-3, Pax-4, Pax-5, Pax-6, Pax-7, Pax-8 和 Pax-9

Pax-6抗体（PAX6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人类和鸟类来源的Pax-6蛋白。抗Pax-6抗体（PAX6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Pax-6在眼睛发育中起着至关重要的作用，对于包括角膜和虹膜在内的眼前段形成至关重要。Pax-6的功能至关重要，因为Pax-6基因突变可导致严重的先天性眼疾，如无虹膜症，其特征是虹膜缺失，可导致严重的视力障碍或失明。Pax-6还与其他前段畸形有关，包括彼得斯异常，其特征是角膜混浊和各种影响眼睛结构的粘连。Pax-6基因编码一种转录调节因子，该因子通过其配对型DNA结合结构域与特定的DNA序列结合，该结构域由两个子结构域组成，它们共同调节对眼睛发育至关重要的基因表达。人类Pax-6基因产生两种可替代剪接的同种型，每种同种型具有不同的结构特征，有助于其调节功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Pax-6 抗体 (PAX6) 参考文献:

  1. 眼部异常中 PAX6 基因替代剪接区的错义突变。  |  Azuma, N., et al. 1999. Am J Hum Genet. 65: 656-63. PMID: 10441571
  2. 在人类脑室下区，神经胶质细胞和祖细胞表达 Sox-2，神经母细胞表达 Pax-6。  |  Baer, K., et al. 2007. Exp Neurol. 204: 828-31. PMID: 17291498
  3. 眼球发育受 SRp55/B52 介导的无眼基因替代剪接的控制。  |  Fic, W., et al. 2007. PLoS One. 2: e253. PMID: 17327915
  4. 蛋白磷酸酶-1能调节控制大脑和眼睛发育的转录因子Pax-6的功能。  |  Yan, Q., et al. 2007. J Biol Chem. 282: 13954-65. PMID: 17374606
  5. 从小鼠, 大鼠和人眼成体睫状体中提取的祖细胞的特征。  |  Xu, H., et al. 2007. Invest Ophthalmol Vis Sci. 48: 1674-82. PMID: 17389499
  6. 人类胶质瘤中 PAX6 基因的突变分析。  |  Pinto, GR., et al. 2007. Genet Mol Res. 6: 1019-25. PMID: 18273794
  7. 对来自阿拉伯半岛的两个无血缘关系的典型遗传性无脑畸形家族的 PAX6 分析。  |  Khan, AO. and Aldahmesh, MA. 2008. Ophthalmic Genet. 29: 145-8. PMID: 18766996
  8. 在蝾螈眼睛发育和晶状体再生过程中pax-6的表达。  |  Del Rio-Tsonis, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 5092-6. PMID: 7761453
  9. PAX6 基因位点的突变可导致包括彼得斯氏畸形在内的多种前节畸形。  |  Hanson, IM., et al. 1994. Nat Genet. 6: 168-73. PMID: 8162071
  10. PAX6突变与无睾症的关系  |  Hanson, IM., et al. 1993. Hum Mol Genet. 2: 915-20. PMID: 8364574
  11. 小鼠晶状体中Pax-6和αB-晶状体蛋白/小热休克蛋白基因的调控。与晶状体特异性区域LSR1和LSR2的相互作用。  |  Gopal-Srivastava, R., et al. 1996. J Biol Chem. 271: 23029-36. PMID: 8798491
  12. PAX-6基因转录的调控：人类大脑中备选启动子的使用。  |  Okladnova, O., et al. 1998. Brain Res Mol Brain Res. 60: 177-92. PMID: 9757029

订购信息

产品名称产品编号规格价格数量收藏夹

Pax-6 抗体 (PAX6)

sc-81649
200 µg/ml
$316.00

Pax-6 (PAX6): m-IgG Fc BP-HRP 套装

sc-528709
200 µg Ab; 10 µg BP
$354.00

Pax-6 (PAX6): m-IgGκ BP-HRP 套装

sc-521177
200 µg Ab, 40 µg BP
$354.00

Pax-6 (PAX6): m-IgG1 BP-HRP 套装

sc-543080
200 µg Ab; 20 µg BP
$354.00

Pax-6 抗体 (PAX6) AC

sc-81649 AC
500 µg/ml, 25% agarose
$416.00

Pax-6 抗体 (PAX6) HRP

sc-81649 HRP
200 µg/ml
$316.00

Pax-6 抗体 (PAX6) FITC

sc-81649 FITC
200 µg/ml
$330.00

Pax-6 抗体 (PAX6) PE

sc-81649 PE
200 µg/ml
$343.00

Pax-6 抗体 (PAX6) Alexa Fluor® 488

sc-81649 AF488
200 µg/ml
$357.00

Pax-6 抗体 (PAX6) Alexa Fluor® 546

sc-81649 AF546
200 µg/ml
$357.00

Pax-6 抗体 (PAX6) Alexa Fluor® 594

sc-81649 AF594
200 µg/ml
$357.00

Pax-6 抗体 (PAX6) Alexa Fluor® 647

sc-81649 AF647
200 µg/ml
$357.00

Pax-6 抗体 (PAX6) Alexa Fluor® 680

sc-81649 AF680
200 µg/ml
$357.00

Pax-6 抗体 (PAX6) Alexa Fluor® 790

sc-81649 AF790
200 µg/ml
$357.00

Is this antibody methanol compatible?

Asked by: Neuro11
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-12-01

how can I get a sample size to test?

Asked by: deborah17
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2021-12-01

Can this antibody be used for detection of Pax-6 of bovine origin?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 12
Date published: 2021-12-01

What secondary is best to use with Pax-6 Antibody (PAX6): sc-81649?

Asked by: AbPolly
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Troponin T-C Antibody (CT3): sc-20025, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-10

I would like to detect Pax-6 in Xenopus? Will this antibody work for me?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2017-01-04
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Rated 5 out of 5 by from Great stainingI've used in mouse embryos at a concentration of 1:200 to stain pallial progenitors. It worked great
Date published: 2018-03-12
Rated 5 out of 5 by from Great staining signalI detected strong staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from Excellent antibodyImmunofluorescence (1:200) works very well on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0) is necessary.
Date published: 2017-10-03
Rated 5 out of 5 by from Great antibodyWorks great on E14.5 mouse brain sample on western blotting. I used 1:1000 dilution over night at 4 C.
Date published: 2017-07-13
Rated 5 out of 5 by from excellentI have used the SAMPLE to test the differentiation of embryonic stem cells for 4 days，The antibody is very specific .
Date published: 2017-02-13
Rated 5 out of 5 by from good sensitivity, trustfulI use this antibody to detect the expression of PAX6 of dental derived Mesenchymal steme cells after certain induction as a neural marker. I conducted the following procedures for a immunocytochemistry( ICC ) detection: 1The cells were fixed in 4% paraformaldehyde for 15minutes, and permeabilized with 0.1% triton-X for 15minutes 2 add 3% H2O2 liquid for 15 minutes,and blocked with 5% BSA for 1h 3 incubated with first antibody(sc-81649) is diluted at 1:150 ,4 °C,overnight 4 washe, incubated with goat anti-mouse HRP conjugate second antibody, DAB coloration 5 microsope , photos taking, positive cells were clear compared to Negative cells
Date published: 2017-01-19
Rated 5 out of 5 by from Great monoclonal antibodyAntibody produced great results by WB and IHC(P). Highly recommended!
Date published: 2017-01-16
Rated 5 out of 5 by from OutstandingOutstanding western blot and ihc staining data; much better than polyclonal alternatives
Date published: 2017-01-01
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Pax-6 抗体 (PAX6) is rated 5.0 out of 5 by 17.
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