Date published: 2025-9-9

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Pax-3 抗体 (F-2): sc-376204

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说明书
  • Pax-3 抗体 F-2 是小鼠单克隆 IgG3Pax-3抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的Pax-3的C-terminus附近的氨基酸387-425之间
  • 推荐用于 mouse, rat, human 和 来源的Pax-3 WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine, bovine, porcine and avian
  • 可用于ChIP的TransCruz试剂(sc-376204 X, 200 µg/0.1 ml)
  • 看 Pax-3/7 (B-5): sc-365843,可查看有标记的 Pax-3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取Pax-3 (F-2): sc-376204免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Pax-3 抗体 (F-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    Pax-3抗体(F-2)是一种IgG3小鼠单克隆Pax-3抗体(也称为PAX3抗体或HUP2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Pax-3蛋白。Pax-3抗体(F-2)为非偶联型抗Pax-3抗体。Pax基因包含与果蝇中参与早期发育编程的基因具有高度同源性的配对结构域。PAX3基因的产物是在早期神经发生过程中表达的DNA结合蛋白。Pax-3是一种同时含有配对结构域和配对型同源结构域的蛋白质。在早期神经发生过程中,Pax-3的表达仅限于发育中的脊髓脑室区的有丝分裂细胞,以及后脑、中脑和间脑的不同区域。在10-12天的胚胎中,Pax-3的表达也见于发育中的脊髓神经节、颅面中胚层和四肢间充质的神经嵴细胞。编码转录因子的MITF和Pax-3基因的突变分别导致Waardenburg综合征II(WSII)和WSI/WSIII。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Pax-3 抗体 (F-2) 参考文献:

    1. 瓦登堡综合症患者的 Pax-3 配对盒基因的人类同源物发生了突变。  |  Tassabehji, M., et al. 1992. Nature. 355: 635-6. PMID: 1347148
    2. 基因表达特征可识别横纹肌肉瘤亚型,并检测出融合 PAX3 与 NCOA1 的新型 t(2;2)(q35;p23)易位。  |  Wachtel, M., et al. 2004. Cancer Res. 64: 5539-45. PMID: 15313887
    3. Pax-3,一种在早期神经发生过程中表达的新型小鼠 DNA 结合蛋白。  |  Goulding, MD., et al. 1991. EMBO J. 10: 1135-47. PMID: 2022185
    4. 自噬依赖性 GILT 和 PAX-3 之间的串扰影响人类黑色素瘤细胞对辐射的敏感性。  |  Hathaway-Schrader, JD., et al. 2018. J Cell Biochem. 119: 2212-2221. PMID: 28857256
    5. PAX3和PAX7作为肌原纤维的上游调节因子。  |  Azhar, M., et al. 2022. J Genet Eng Biotechnol. 20: 143. PMID: 36251225
    6. Pax3 的缺失会导致发育中小鼠耳蜗中黑色素细胞的减少。  |  Udagawa, T., et al. 2024. Sci Rep. 14: 2210. PMID: 38278860
    7. 分离 PAX3 基因转录本的两种异构体及其在人类成人组织中的特异性替代表达。  |  Tsukamoto, K., et al. 1994. Hum Genet. 93: 270-4. PMID: 7545913
    8. [电针血清对培养的多裂肌卫星细胞增殖以及Pax-7、MyoD和p-Akt表达的影响]。  |  Goulding, M., et al. 1994. Development. 120: 957-71. PMID: 7600971
    9. 七个 PAX 基因的染色体定位和一个新家族成员 PAX-9 的克隆。  |  Stapleton, P., et al. 1993. Nat Genet. 3: 292-8. PMID: 7981748
    10. 人类 PAX3 基因成对结构域的突变会导致克莱因-瓦登堡综合征(WS-III)和 I 型瓦登堡综合征(WS-I)。  |  Hoth, CF., et al. 1993. Am J Hum Genet. 52: 455-62. PMID: 8447316
    11. 瓦登堡综合征基因 MITF 和 PAX3 之间的表观关系。  |  Watanabe, A., et al. 1998. Nat Genet. 18: 283-6. PMID: 9500554
    12. 高迁移率组蛋白1通过增加miR-342-5p的表达,下调Pax-7的合成,从而阻碍骨骼肌再生。  |  Mansouri, A. 1998. Crit Rev Oncog. 9: 141-9. PMID: 9973247

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Pax-3 抗体 (F-2)

    sc-376204
    200 µg/ml
    $316.00

    Pax-3 (F-2) 中和勝肽

    sc-376204 P
    100 µg/0.5 ml
    $68.00

    Pax-3 抗体 (F-2) X

    sc-376204 X
    200 µg/0.1 ml
    $316.00

    What application is the blocking peptide sc-376204 P appropriate for?

    Asked by: Trav11
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 5 out of 5 by from Works well in western blotThe antibody detects PAX-3 in melanoma cells at the expected molecular size.
    Date published: 2020-09-18
    Rated 4 out of 5 by from Good ProductThis product works good in IP and WB with mouse brain tissue.
    Date published: 2019-06-13
    Rated 4 out of 5 by from Good antibodyPax3 antibody worked well in Western blotting analysis at 1:500 dilution using mouse tissue lysate.
    Date published: 2018-09-22
    Rated 1 out of 5 by from Not good for IFTest IF on cryosetions from mouse embryonic cerebellum, no specific signal were detected.
    Date published: 2017-10-18
    Rated 1 out of 5 by from Did not work on mouse brain tissue (Thalamus)Immunofluorescence (1:200) did not work on frozen section from 4% PFA fixed embryonic mouse brain tissue (Thalamus). 3-5 min retrieval with citric buffer (PH6.0).
    Date published: 2017-10-11
    Rated 5 out of 5 by from very goodi bought this a month ago ,it is very good single band
    Date published: 2017-08-03
    Rated 5 out of 5 by from Good Western blot data of PaxGood Western blot data of Pax-3 expression in non-transfected and mouse Pax-3 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-01-02
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    Pax-3 抗体 (F-2) is rated 3.6 out of 5 by 7.
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