PARP1 Double Nickase Plasmid (h): sc-400046-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PARP1 Double Nickase Plasmid (h) and PARP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PARP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARP1 Antibody (F-2): sc-8007
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP1 Double Nickase Plasmid (h) | sc-400046-NIC | 20 µg | $410.00 | |||
PARP1 Double Nickase Plasmid (h2) | sc-400046-NIC-2 | 20 µg | $410.00 |
PARP1 encodes poly(ADP-ribose) polymerase 1, a nuclear enzyme that detects DNA single-strand breaks and catalyzes poly(ADP-ribosyl)ation of itself and other chromatin-associated proteins to coordinate DNA repair and chromatin remodeling. PARP1 functions prominently in base excision repair and single-strand break repair, and interfaces with replication stress responses, transcriptional regulation, and genome stability pathways through protein recruitment and chromatin relaxation. Altered PARP1 activity or expression is associated with defects in DNA damage signaling, mutational accumulation, and dysregulated cell survival under genotoxic stress, making it a widely studied node in oncogenic transformation and therapy response models. PARP1 is also investigated in neurodegeneration and inflammation contexts where persistent DNA damage and PAR metabolism can influence cell fate and gene expression programs.
PARP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PARP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PARP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PARP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PARP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.