Date published: 2026-10-19
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PARP1 Antibody (5A5) - Western Blotting - Image 416267
PARP1（5A5）抗体：sc-56197。通过近红外Western blot分析HeLa（A）、Jurkat（B）、THP-1 （C）和 Raji（D）细胞总裂解物中 PARP-1 的表达。使用的检测试剂：m-IgG Fc BP-CFL 790：sc-533658。使用 UltraCruz® 阻断试剂进行阻断：sc-516214。
PARP1（5A5）抗体：sc-56197。通过近红外Western blot分析HeLa（A）、Jurkat（B）、THP-1 （C）和 Raji（D）细胞总裂解物中 PARP-1 的表达。使用的检测试剂：m-IgG Fc BP-CFL 790：sc-533658。使用 UltraCruz® 阻断试剂进行阻断：sc-516214。

PARP1 抗体 (5A5): sc-56197

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说明书
  • PARP1 抗体 5A5 是小鼠单克隆 IgG1, 在32篇文献中引用，提供 200 µg/ml
  • 针对human来源的PARP-1片段
  • 推荐用于 mouse, rat 和 human 来源的PARP-1 WB, IP 和 IF检测
  • 看 PARP1 (F-2): sc-8007，可查看有标记的 PARP1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP是PARP1 Antibody (5A5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与PARP1 Antibody (5A5) 搭配使用（请参阅下面的订购信息）。
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関連項目

附加PARP抗体，包括pADPr, PARP-1, PARP-10, PARP-14, PARP-2, PARP-3和PARP-4

多聚（ADP-核糖）聚合酶-1（PARP-1），也称为PARP，是一种核DNA结合锌指蛋白，可影响DNA修复、DNA复制、染色质结构调制和细胞凋亡。作为对遗传毒性应激的响应，PARP-1催化ADP-核糖单位从NAD（+）转移到包括染色质在内的许多受体分子上。PARP-1识别DNA链中断，并能与RNA形成复合物并负调控转录。放线菌素D和依托泊苷依赖性诱导的半胱天冬酶介导PARP-1裂解成p89片段，该片段进入细胞质。凋亡诱导因子（AIF）从线粒体到细胞核的转位依赖于PARP-1，并且是PARP-1依赖性细胞死亡所必需的。PARP-1的缺乏导致由于染色体融合和异倍体频率的增加而引起的染色体不稳定性，这表明多聚（ADP-核糖基）化有助于有效维持基因组完整性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PARP1 抗体 (5A5) 参考文献:

  1. E3泛素连接酶WWP2对PARP1泛素化和降解的选择性靶向调节异丙肾上腺素诱导的心脏重塑。  |  Zhang, N., et al. 2020. Cell Death Differ. 27: 2605-2619. PMID: 32139900
  2. 癌生物学中的核仁和PARP1。  |  Engbrecht, M. and Mangerich, A. 2020. Cancers (Basel). 12: PMID: 32640701
  3. 89 kDa PARP1 裂解片段可作为细胞质 PAR 载体，诱导 AIF 介导的细胞凋亡。  |  Mashimo, M., et al. 2021. J Biol Chem. 296: 100046. PMID: 33168626
  4. PARP1在神经退行性疾病和衰老中的作用。  |  Mao, K. and Zhang, G. 2022. FEBS J. 289: 2013-2024. PMID: 33460497
  5. SIRT2对PARP1的去乙酰化依赖性调节决定了氧化应激诱导的血管损伤中PARP1的泛素化。  |  Zhang, N., et al. 2021. Redox Biol. 47: 102141. PMID: 34555594
  6. 持续激活的PARP1介导信号诱导的基因表达。  |  Cohen-Armon, M. 2022. Cells. 11: PMID: 35563882
  7. PARP1：染色质重塑和转录的联络点。  |  Zong, W., et al. 2022. Cancers (Basel). 14: PMID: 36077699
  8. PARP1 PROTAC作为一种新型策略，通过促进p53阳性乳腺癌中的铁死亡来对抗PARP抑制剂耐药性。  |  Li, G., et al. 2022. Biochem Pharmacol. 206: 115329. PMID: 36309080
  9. PARP1抑制剂可保护小鼠免受日本脑炎病毒感染。  |  Desingu, PA., et al. 2023. Cell Rep. 42: 113103. PMID: 37676769
  10. Nimbolide 以 RNF114 为靶标，诱导 PARP1 诱捕和 BRCA 基因突变癌症的合成致死率。  |  Li, P., et al. 2023. Sci Adv. 9: eadg7752. PMID: 37878693

订购信息

产品名称产品编号规格价格数量收藏夹

PARP1 抗体 (5A5)

sc-56197
200 µg/ml
$322.00

PARP1 (5A5): m-IgG Fc BP-HRP 套装

sc-551932
200 µg Ab; 10 µg BP
$361.00

Are there any issues with using sc-56197: PARP-1 (5A5) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56197 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by cxbbb from 很好用！抗体特异性高！没有多余的非特异性条带！在用过的PARP1抗体中算是效果比较理想的！非常满意！好好好好好
Date published: 2025-02-19
Rated 5 out of 5 by Trav11 from High QualityThe PARP-1 Antibody (5A5): sc-56197 provides great Western blot data, showing clear banding with minimal background noise. This antibody has also been cited several times for further verification.
Date published: 2017-02-02
Rated 5 out of 5 by zhangxiaodou from work wellthe antibody workes well for dection of apoptosis in WB
Date published: 2016-12-23
Rated 5 out of 5 by dingding from very goodI bought this product a month ago, and I feel satisfaction.
Date published: 2016-12-02
Rated 4 out of 5 by Xing_Huang from Worked well in WBWorked well in WB
Date published: 2015-11-17
Rated 5 out of 5 by CJ from Single strong band for WB in Jurkat wholeSingle strong band for WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by MC from Performed well in Jurkat (A)Performed well in Jurkat (A), HeLa (B) and K-562 (C) nuclear extracts. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by TO from Good specificity and signal strength for WB in nonGood specificity and signal strength for WB in non-transfected 293T(A), mouse PARP-1 transfected 293T(B) and Jurkat (C) whole cell lysates. -SCBT QC
Date published: 2015-01-28
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PARP1 抗体 (5A5) is rated 4.9 out of 5 by 9.
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