Date published: 2025-9-6

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pan Ras 抗体 (C-4): sc-166691

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说明书
  • pan Ras 抗体 C-4 是小鼠单克隆 IgG2b κ,pan Ras抗体, 在43篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长N-Ras的氨基酸1-189
  • pan Ras 抗体 (C-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 N-Ras, H-Ras and K-Ras p21; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗pan Ras抗体(C-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取pan Ras (C-4): sc-166691免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 pan Ras 抗体 (C-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与pan Ras 抗体 (C-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Pan Ras抗体(C-4)是一种IgG2b κ型小鼠单克隆Pan Ras抗体,能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组化(IHC(P))和酶联免疫吸附测定(ELISA)检测来自小鼠、大鼠和人类的Pan Ras蛋白。Pan Ras抗体(C-4)既有非偶联形式的抗Pan Ras抗体,也有多种偶联形式的抗Pan Ras抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。哺乳动物的c-H-、c-K-和N-Ras原癌基因编码鸟嘌呤核苷酸结合蛋白,这些蛋白在脊椎动物细胞中普遍表达。c-H-和c-K-Ras是最初从大鼠肉瘤病毒的Harvey和Kirsten株中分离出的v-H和v-K-Ras序列的细胞同源物。Ras编码的蛋白以高亲和力结合GDP和GTP,并具有低水平的内在GTP酶活性,该活性可以通过与细胞质GTP酶激活蛋白(GAP)(Ras p21功能的潜在效应物)相互作用而刺激超过100倍。负责GTP结合和水解的结构域内氨基酸12、13、59和61的点突变会激活Ras蛋白至其致癌形式,并阻断GTP酶活性受GAP刺激的能力。已经鉴定出几种具有GAP活性的额外蛋白质,并显示它们与p21 Ras或Ras基因家族的其他成员相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

pan Ras 抗体 (C-4)

sc-166691
200 µg/ml
$316.00

pan Ras (C-4): m-IgGκ BP-HRP 套装

sc-521712
200 µg Ab, 40 µg BP
$354.00

pan Ras (C-4): m-IgG2b BP-HRP 套装

sc-548956
200 µg Ab; 10 µg BP
$354.00

pan Ras 抗体 (C-4) AC

sc-166691 AC
500 µg/ml, 25% agarose
$416.00

pan Ras 抗体 (C-4) HRP

sc-166691 HRP
200 µg/ml
$316.00

pan Ras 抗体 (C-4) FITC

sc-166691 FITC
200 µg/ml
$330.00

pan Ras 抗体 (C-4) PE

sc-166691 PE
200 µg/ml
$343.00

pan Ras 抗体 (C-4) Alexa Fluor® 488

sc-166691 AF488
200 µg/ml
$357.00

pan Ras 抗体 (C-4) Alexa Fluor® 546

sc-166691 AF546
200 µg/ml
$357.00

pan Ras 抗体 (C-4) Alexa Fluor® 594

sc-166691 AF594
200 µg/ml
$357.00

pan Ras 抗体 (C-4) Alexa Fluor® 647

sc-166691 AF647
200 µg/ml
$357.00

pan Ras 抗体 (C-4) Alexa Fluor® 680

sc-166691 AF680
200 µg/ml
$357.00

pan Ras 抗体 (C-4) Alexa Fluor® 790

sc-166691 AF790
200 µg/ml
$357.00

The polyclonal predecessor of this antibody (https://www.scbt.com/scbt/product/k-ras-2b-antibody-c-19) binds to the c-terminus of RAS. Does this also bind to the c-terminus?

Asked by: Jonathan123
Thank you for your question. The exact epitope for this monoclonal antibody has not yet been epitope mapped, so we do not have this information. It was raised against full length Ras of human origin. Please contact our Technical Service Department and we would be glad to discuss the reactivity of this antibody. You can reach us by email at scbt@scbt.com, or use the live chat on our website.
Answered by: Tech Service
Date published: 2017-06-09

What is the recommended secondary reagent to use with pan Ras (C-4): sc-166691 mouse monoclonal primary?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with pan Ras (C-4): sc-166691, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-10
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Rated 4 out of 5 by from Good bandsI used it to detect the RAS proteins in A549 and HeLa lysates
Date published: 2021-03-17
Rated 5 out of 5 by from Nice signal by WB on mouse colon extractsWe used the ab by WB on mouse colon protein extracts and obtained a nice signal at the expected MW using a 1/500 dilution ( in TBST-5% non fat milk) on 20 ug of protein and with regular ECL
Date published: 2017-10-27
Rated 5 out of 5 by from Strong clean Western bandsWorks well for Western in Molt-4 cell line. Clean blot.
Date published: 2017-10-11
Rated 5 out of 5 by from work well in western blot at 1:200,ov90 cellThe bands are strong and clear.The antibody works really well
Date published: 2017-09-20
Rated 5 out of 5 by from Excellent for WBit works excellent for WB in our lab, will keep usinig it
Date published: 2017-09-18
Rated 4 out of 5 by from not very specific for WBthis antibody shows weak target band acompanies nonspecific bands in WB in human lung cencer cell lysates
Date published: 2017-08-02
Rated 5 out of 5 by from very goodEasy to get good band by western blot. 1:1000 dilution. loading 20ug.
Date published: 2017-06-06
Rated 5 out of 5 by from very goodWestern blot, AsPC-1 & KPC cell lines, loading 30ug, 1:1000 dilution, strong band at the right size.
Date published: 2017-04-20
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pan Ras 抗体 (C-4) is rated 4.8 out of 5 by 15.
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