Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
KRAS Antibody (F234) - Western Blotting - Image 4199
K-Ras 抗体 (F234): sc-30. 对 KNRK 全细胞裂解液中 K-Ras 的表达进行 Western 印迹分析.
K-Ras 抗体 (F234): sc-30. 对 KNRK 全细胞裂解液中 K-Ras 的表达进行 Western 印迹分析.

KRAS 抗体 (F234): sc-30

3.9(31)
寫評論提問

说明书
  • KRAS 抗体 F234 是小鼠单克隆 IgG2a κ，KRAS抗体, 在459篇文献中引用，规格为100 µg/ml
  • 针对重组的包含rat起源的54-189的氨基酸K-Ras的抗体
  • 推荐用于 mouse, rat 和 human 来源的c-K-Ras and v-K-Ras WB, IP 和 IF检测; 可能与 c-H-Ras and c-N-Ras产生交联反应
  • 看 pan Ras (C-4): sc-166691，可查看有标记的 KRAS 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取KRAS (F234): sc-30的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是KRAS Antibody (F234) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与KRAS Antibody (F234) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Ras抗体，包括Ras-GRF1, κ B-Ras1, κ B-Ras2, E-Ras, H-Ras, N-Ras, R-Ras, pan Ras, Ras GAP, Ras2和K-Ras

K-Ras抗体（F234）是一种IgG2a κ小鼠单克隆K-Ras抗体（也称为KRAS抗体或c-K-Ras抗体），可通过WB、IP和IF检测来自小鼠、大鼠和人类的K-Ras蛋白。K-Ras抗体（F234）既可以是非偶联的抗K-Ras抗体，也可以是多种偶联形式的抗K-Ras抗体。哺乳动物Ras（也称为v-Ha-Ras、Harvey大鼠肉瘤病毒致癌基因同源物、HRAS1、K-Ras、N-Ras、RASH1或c-bas/has）基因家族由Harvey和Kirsten Ras基因（c-H-Ras1和c-K-Ras2）、每个基因的一个无活性假基因（c-H-Ras2和c-K-Ras1）以及N-Ras基因组成。三种Ras致癌基因H-Ras、K-Ras和N-Ras编码具有GTP/GDP结合和GTP酶活性的蛋白质。Ras蛋白在GDP结合的无活性形式和GTP结合的活性形式之间交替被Guanine核苷交换因子（GEF）激活，被GTP酶激活蛋白（GAP）失活。Ras的命名来源于与C型哺乳动物逆转录病毒Harvey株鼠类肉瘤病毒(HaMSV)致癌序列的克隆DNA片段同源的人类DNA序列的特征。在正常情况下，Ras家族成员影响基于亚细胞膜分离的信号传导系统中的细胞生长和分化。致癌Ras可以解除控制细胞增殖和凋亡的过程。GTP水解偶联信号转导中继蛋白Ras超家族可分为Ras、Rho、Rab和ARF家族。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

KRAS 抗体 (F234) 参考文献:

  1. Ras 的阴暗面：细胞凋亡的调控。  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
  2. 前列腺癌进展过程中的 Ras 信号传导。  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
  3. 人类 RAS 超家族蛋白和相关 GTP 酶。  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
  4. 肿瘤进展和转移中的致癌 Ras。  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
  5. Ras 质膜信号平台。  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
  6. 高尔基体上的 Ras 信号传导。  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
  7. 分区化的 Ras/MAPK 信号传导。  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
  8. Ras, PI(3)K 和 mTOR 信号控制着肿瘤细胞的生长。  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
  9. 人类 DNA 中与哺乳动物肉瘤逆转录病毒转化基因相关的三个不同基因。  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

订购信息

产品名称产品编号规格价格数量收藏夹

KRAS 抗体 (F234)

sc-30
100 µg/ml
$316.00

KRAS (F234): m-IgGκ BP-HRP 套装

sc-520388
100 µg Ab, 40 µg BP
$354.00

Is this antibody specific for KRAS 4A or KRAS 4B or does it recognise both isoforms?

Asked by: CristinaM
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2022-03-14

对于KRAS G12D突变的情况。这个抗体能反映出 G12D与非突变的差异吗

Asked by: HRZ_Richard
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 9
Date published: 2022-03-14

Can this SC-30 antibody be used for flow cytometry purposes? And if so, what protocol would you recommend using (in terms of obtaining the appropriate amount of antibody for flow sorting human cancer cells)? 

Asked by: MA08
Thank you for your question. Here is a link to the protocol we use for Flow Cytometry: https://www.scbt.com/scbt/resources/protocols/flow-cytometry If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-16

Can anti-kras antibodies be used during immunofluorescence assays of cell cultures without a permeabilization step? In other words, can k-ras be detected and recognized from outside the cell?

Asked by: cicak
Thank you for your question. K-Ras is generally a cytoplasmic membrane protein, which requires the antibody to be able to enter the cell. We recommend methanol fixation as this partially permeabilizes the cell allowing for good detection. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-05-08

IS THIS CAN BE USE FOR IHC AT HUMAN TISSUE SAMPLE (PARAFINATED)

Asked by: DIKSHA
Thank you for your question. We have not tested this mouse monoclonal, K-Ras (F234): sc-30, for IHC on formalin-fixed paraffin-embedded tissue sections. However, this particular clone has been cited in several publications for this research application so it may be suitable. As a reference, please refer to the following product citations on PubMed, PMID's: 12727811, 10884918, 18973197 and 19545448.
Answered by: Technical Support
Date published: 2017-01-10

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
No, since this antibody doesn't have any direct conjugates available, it would not be recommended for IHC in mouse tissue. See pan Ras (C-4): sc-166691 for K-Ras antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Tech Service 5
Date published: 2016-12-16

hi, what dilution of this antibody to be used for western blot?

Asked by: moni
Thank you for your question. We recommend using this mouse monoclonal, K-Ras Antibody (F234): sc-30, at a starting dilution of 1:200 (range of 1:100-1:1,000) for Western blotting. This information is also provided on our product datasheet. A link is posted below. https://datasheets.scbt.com/sc-30.pdf
Answered by: Technical Support
Date published: 2020-02-06
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_8
  • loc_zh_CN, sid_30, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great signalI have used this antibody many time in blots. Works all the time. I recommend.
Date published: 2024-05-30
Rated 5 out of 5 by from Great K-Ras antibody for Western Blot applicationClone F234 is a classic K-ras antibody raised against the rat protein, but works well with human K-Ras as well. Antibody showed a strong 21 KDa Western Blot band with transfected lysate sc-111225, that over-expressed human K-Ras (right lane) Detected with mouse IgG kappa binding protein (m-IgGκ BP): sc-516102 GenBank Sequence Reference: BC013572.2 -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from K-Ras 抗体 (F234) 做WB效果超级棒K-Ras 抗体 (F234)买来做WB的，和其他的品牌比起来效果太好了。超赞，一如既往的支持Santa Cruiz抗体，科研工作者的首选。
Date published: 2021-11-09
Rated 3 out of 5 by from good for overexpressed proteinI used it with milk overnight at 4oC but detected only the overexpressed protein from A549 lung adenocarcinoma cells
Date published: 2021-03-17
Rated 4 out of 5 by from Figura 2 - J, KWestern blot analysis of KRAS in three mutant KRAS lung adenocarcinoma cell lines (H1792.604, H2009, and H358).
Date published: 2019-02-22
Rated 4 out of 5 by from A very specific antibodyI requested the free sample and the result was good.
Date published: 2018-07-29
Rated 1 out of 5 by from Ne détecte pas la protéineSur HEK transfectées pour surexprimer K-Ras4B, aucun signal détecté dans les extraits totaux (WB réalisé en PBS). Sur cette même manip, l'IP réalisée avec cet anticorps n'a permis de détecter aucun signal de Ras.
Date published: 2018-03-18
Rated 3 out of 5 by from KRas WBWe bought this antibody to do Western blots for endogenous KRas in HEK and HeLa cells. Endogenous KRas levels in those cell lines are rather low so while we can detect it, there are several strong non-specific protein bands, which is slightly dispappointing. Otherwise, not a bad antibody.
Date published: 2018-03-08
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_30, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
KRAS 抗体 (F234) is rated 3.9 out of 5 by 31.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_31
  • loc_zh_CN, sid_30, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。