Date published: 2025-9-6

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CDKN1B/Kip1 p27 抗体 (0.N.491): sc-71813

4.6(11)
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说明书
  • CDKN1B/Kip1 p27抗体(0.N.491)是小鼠单克隆IgG1 κ, 在13篇文献中引用,规格为200 µg/ml
  • 针对mouse来源的全长p27 Kip1
  • 推荐用于 mouse, rat 和 human 来源的p27 Kip1 WB, IP, IF 和 IHC(P)检测; 不会与 other mitotic inhibitors产生交联反应
  • 看 CDKN1B/Kip1 p27 (F-8): sc-1641,可查看有标记的 CDKN1B/Kip1 p27 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 CDKN1B/Kip1 p27 抗体 (0.N.491) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CDKN1B/Kip1 p27 抗体 (0.N.491) 组合成套装提供。 (请参阅下面的订购信息)。

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p27 Kip1抗体(0.N.491)是一种小鼠单克隆IgG1抗体,可通过多种应用检测小鼠、大鼠和人类样本中的p27 Kip1,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学。p2 7 Kip1作为细胞周期进展的负调控因子发挥着至关重要的作用,特别是在G1期,p27 Kip1通过与D型细胞周期蛋白和Cdk4结合来抑制细胞周期蛋白依赖性激酶(Cdks)的活性,从而阻止向S期的过渡。这种调节对于维持正常的细胞生长和分裂至关重要,特别是对于TGFβ等外部信号的反应,这些信号可以诱导G1期停滞。p27 Kip1能够调节细胞周期的进展,这凸显了p27 Kip1在肿瘤抑制中的重要性,因为失调会导致细胞无序增殖和癌症发展。抗p27 Kip1抗体(0.N.491)是研究人员研究细胞周期动力学和各种疾病分子机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN1B/Kip1 p27 抗体 (0.N.491)

sc-71813
200 µg/ml
$316.00

CDKN1B/Kip1 p27 (0.N.491): m-IgG Fc BP-HRP 套装

sc-539413
200 µg Ab; 10 µg BP
$354.00

CDKN1B/Kip1 p27 (0.N.491): m-IgG1 BP-HRP 套装

sc-541477
200 µg Ab; 20 µg BP
$354.00

Can p27 (0.N.491): sc-71813 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, p27 (0.N.491): sc-71813 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good for detecting mouse and human p27 by WBGood for detecting p27 in mouse (MEF) and human (U2OS) cells at 1:500 dilution.
Date published: 2018-11-14
Rated 5 out of 5 by from It is useful for Western blot!I tested this antibody for TPA or DMBA experiments. While the cells occur apoptosis, this antibody worked, showed its activation.
Date published: 2018-07-05
Rated 5 out of 5 by from Great antibody! worked in my hand.A single strong band was observed using this antibody in various WCLs by western. The signals are always strong and clear.
Date published: 2017-09-11
Rated 5 out of 5 by from good jobI used it at a dilution of 1:1000 for WB incubated in 4℃ overnight, and the results were quite good! Clean band with little background for WB in mcf7 and 293t whole cell lysates!
Date published: 2016-12-30
Rated 5 out of 5 by from Excellent antibodiyA good antibody!!!This antibody works very well in western blot detection in the H1299,hela,u2os cells.
Date published: 2016-12-27
Rated 5 out of 5 by from Western blot of this antibodyi use this antibody to select high expression p27 celline,at last i got the different exprsssion levels band,very useful!
Date published: 2016-12-13
Rated 5 out of 5 by from strong signal!I used it at a dilution of 1:500 for WB, and the results were great!Clean band with little background for WB in h1299, sw620, hela, u2os whole cell lysates!!
Date published: 2016-12-07
Rated 4 out of 5 by from CleanClean, singular band observed in western blot analysis of Jurkat and human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-25
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CDKN1B/Kip1 p27 抗体 (0.N.491) is rated 4.6 out of 5 by 11.
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