Date published: 2025-9-7
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p22-phox Antibody (44.1) - Western Blotting - Image 351981
p22-phox 抗体 (44. 1): sc-130550. NCI-H929 全细胞裂解液 (A) 和人脾组织提取物 (B) 中 p22-phox 表达的 Western 印迹分析.
p22-phox 抗体 (44. 1): sc-130550. NCI-H929 全细胞裂解液 (A) 和人脾组织提取物 (B) 中 p22-phox 表达的 Western 印迹分析.

p22-phox 抗体 (44.1): sc-130550

4.4(5)
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说明书
  • p22-phox抗体(44.1)是小鼠单克隆IgG2a κ, 在21篇文献中引用，规格为200 µg/ml
  • 与human的p22-phox相比，具有与氨基酸29-33 and 182-188的表位图谱
  • 抗-p22-phox 抗体 (44.1) 推荐用于 WB, IP 和 FCM，检测mouse, rat 和human 来源的 p22-phox
  • 抗p22-phox抗体（44.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p22-phox (44.1): sc-130550的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是p22-phox Antibody (44.1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p22-phox Antibody (44.1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p22-phox抗体（44.1）是一种IgG2aκ小鼠单克隆p22-phox抗体（也称为CYBA抗体），可通过WB、IP和FCM检测小鼠、大鼠和人源的p22-phox。p22-phox抗体（44.1）既可作为非偶联抗p22-phox抗体形式存在，也可作为多种偶联形式的抗p22-phox抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Mox1和糖蛋白gp91-phox是密切相关的蛋白质，是NADPH氧化酶的重要组成部分。超氧化物生成的NADPH氧化酶存在于吞噬细胞、神经上皮体、血管平滑肌细胞和内皮细胞中。它包括含有两个亚基gp91-phox和p22-phox的膜结合黄素细胞色素，以及胞质蛋白p47-phox和p67-phox。在NADPH氧化酶激活过程中，p47-phox和p67-phox迁移到质膜，在那里它们与黄素细胞色素细胞色素b558结合形成活性酶复合物。p22-和gp91-phox亚基也作为表面O2传感器，响应缺氧条件启动细胞信号传导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p22-phox 抗体 (44.1)

sc-130550
200 µg/ml
$316.00

p22-phox (44.1): m-IgG Fc BP-HRP 套装

sc-525740
200 µg Ab; 10 µg BP
$354.00

p22-phox (44.1): m-IgG2a BP-HRP 套装

sc-546390
200 µg Ab; 10 µg BP
$354.00

p22-phox 抗体 (44.1) AC

sc-130550 AC
500 µg/ml, 25% agarose
$416.00

p22-phox 抗体 (44.1) HRP

sc-130550 HRP
200 µg/ml
$316.00

p22-phox 抗体 (44.1) FITC

sc-130550 FITC
200 µg/ml
$330.00

p22-phox 抗体 (44.1) PE

sc-130550 PE
200 µg/ml
$343.00

p22-phox 抗体 (44.1) Alexa Fluor® 488

sc-130550 AF488
200 µg/ml
$357.00

p22-phox 抗体 (44.1) Alexa Fluor® 546

sc-130550 AF546
200 µg/ml
$357.00

p22-phox 抗体 (44.1) Alexa Fluor® 594

sc-130550 AF594
200 µg/ml
$357.00

p22-phox 抗体 (44.1) Alexa Fluor® 647

sc-130550 AF647
200 µg/ml
$357.00

p22-phox 抗体 (44.1) Alexa Fluor® 680

sc-130550 AF680
200 µg/ml
$357.00

p22-phox 抗体 (44.1) Alexa Fluor® 790

sc-130550 AF790
200 µg/ml
$357.00

Hello, You state that this clone can recognize mouse p22. Do you have any example immunoblot or flow cytometry data for this antibody on mouse cells? Thanks, David

Asked by: David M
Thank you for your question. We have not yet tested this mouse monoclonal antibody, p22-phox (44.1): sc-130550, in mouse or rat samples. However, the antibody is expected to cross-react with mouse and rat based on sequence identity with the antigen used to develop the antibody. We do warranty the antibody for detection of p22-phox of mouse, rat or human origin.
Answered by: Technical Support
Date published: 2018-01-12

Can p22-phox (44.1): sc-130550 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, p22-phox (44.1): sc-130550 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good detection in protein extract from human bloodI tested the antibody at 1:1000 dilution in Everyblot solution (by Biorad), by overnight incubation at 4°C. In this conditions, I obtained a very good signal in samples from human blood, while I was not able to detect the signal in human immortalized fibroblasts.
Date published: 2024-12-10
Rated 5 out of 5 by from Perfect for FACS applicationWe bought the sc-130550 PE (i.e. the PE coupled clone 44.1) and tested it on both PBMCs from a normal donor or from a p22phox mutated patient. The signal is absolutely perfect and specific since only the control shows a nice and strong peak.
Date published: 2017-04-06
Rated 4 out of 5 by from Published WB data with human and hamster H661Published WB data with human and hamster H661, CHO-91-47-67, CHO-K1 - SCBT Publication Review
Date published: 2015-01-26
Rated 5 out of 5 by from Robust band present in western blot analysisRobust band present in western blot analysis of p22-phox expression in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-01-20
Rated 4 out of 5 by from Great intracellular FCM data of fixed andGreat intracellular FCM data of fixed and permeabilized THP-1 cells. -SCBT QC
Date published: 2013-12-04
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p22-phox 抗体 (44.1) is rated 4.4 out of 5 by 5.
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