Date published: 2025-9-6
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CDKN2B/p15 INK4B Antibody (D-12) - Western Blotting - Image 353251
p15 INK4B 抗体 (D-12): sc-271791. Caco-2 全细胞裂解液中 p15 INK4B 表达的 Western 印迹分析.
CDKN2B/p15 INK4B Antibody (D-12) - Western Blotting - Image 353251
p15 INK4B 抗体 (D-12): sc-271791. Caco-2 全细胞裂解液中 p15 INK4B 表达的 Western 印迹分析.
CDKN2B/p15 INK4B Antibody (D-12) - Western Blotting - Image 136996
p15 INK4B 抗体 (D-12): sc-271791. 未转染 293T: sc-117752 (A), 转染人 p15 INK4B 的 293T: sc-129373 (B), A549 (C) 和 HeLa (D) 全细胞裂解液中 p15 INK4B 表达的 Western 印迹分析.
p15 INK4B 抗体 (D-12): sc-271791. Caco-2 全细胞裂解液中 p15 INK4B 表达的 Western 印迹分析.

CDKN2B/p15 INK4B 抗体 (D-12): sc-271791

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寫評論提問

说明书
  • CDKN2B/p15 INK4B 抗体 D-12 是小鼠单克隆 IgG2a κ，CDKN2B/p15 INK4B抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的p15的C-terminus的氨基酸103-137之间
  • CDKN2B/p15 INK4B 抗体 (D-12) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 p15 INK4B
  • 抗CDKN2B/p15 INK4B抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CDKN2B/p15 INK4B (D-12): sc-271791的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是CDKN2B/p15 INK4B Antibody (D-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CDKN2B/p15 INK4B Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加p15 INK4B抗体，包括PCLAF

p15 INK4B抗体（D-12）是一种IgG2a κ型小鼠单克隆p15 INK4B抗体（也被称为CDKN2B抗体、MTS2抗体、p15ink4b抗体或p15抗体），可以通过WB、IP、IF和ELISA等方法检测来自人类的p15 INK4B蛋白。p15 INK4B抗体（D-12）既有非偶联形式的抗p15 INK4B抗体，也有多种偶联形式的抗p15 INK4B抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞周期的正常进程受细胞周期蛋白依赖性蛋白激酶Cdk4和Cdk6的控制，这两种激酶受到有丝分裂抑制蛋白p16 INK4A的抑制。p16 INK4A家族中的一个独立成员被命名为p15 INK4B（也被称为p15、INK4B、CDK4I、TP15或MTS2）。在经TGFβ处理的人角质形成细胞中，p15 INK4B的表达上调约30倍，这表明p15 INK4B可能作为TGFβ介导的细胞周期停滞的效应物。编码p15 INK4B（CDKN2B）的基因已被定位到9p21.3染色体上，该位置与p16 INK4A基因相邻，是人类肿瘤中染色体异常频发的位点。有研究表明，p15 INK4B可能通过抑制Cdk4和Cdk6激酶来作为TGFβ介导的细胞周期停滞的效应物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CDKN2B/p15 INK4B 抗体 (D-12) 参考文献:

  1. 骨髓增生异常综合征中 p15（INK4B）基因的甲基化：可在诊断早期或疾病进展过程中检测到，并与白血病转化体高度相关。  |  Tien, HF., et al. 2001. Br J Haematol. 112: 148-54. PMID: 11167795
  2. 急性白血病和骨髓增生异常综合征中 p15(INK4B) 基因的高甲基化。  |  Chen, H. and Wu, S. 2002. Chin Med J (Engl). 115: 987-90. PMID: 12150726
  3. 抗表皮生长因子受体单克隆抗体 225 能上调 p27（KIP1）和 p15（INK4B），并诱导口腔鳞癌细胞系 G1 停滞。  |  Kiyota, A., et al. 2002. Oncology. 63: 92-8. PMID: 12187077
  4. p15(INK4b)和E-cadherin基因的甲基化与急性髓性白血病的不良预后独立相关。  |  Shimamoto, T., et al. 2005. Leuk Res. 29: 653-9. PMID: 15863205
  5. 套细胞淋巴瘤中CDK抑制剂[p16(INK4a), p15(INK4b), p14(ARF)]的表观遗传学改变和基因组缺失的差异效应。  |  Hutter, G., et al. 2006. Genes Chromosomes Cancer. 45: 203-10. PMID: 16258956
  6. 组蛋白去乙酰化酶 3 通过与 Sp1 相互作用抑制 p15（INK4b）和 p21（WAF1/cip1）的转录。  |  Huang, W., et al. 2006. Biochem Biophys Res Commun. 339: 165-71. PMID: 16298343
  7. INK4b-ARF-INK4a 抑癌基因位点的调控：人人为我还是我为人人。  |  Gil, J. and Peters, G. 2006. Nat Rev Mol Cell Biol. 7: 667-77. PMID: 16921403
  8. p15(INK4b)在小鼠淋巴细胞发育和肿瘤发生中起着至关重要的作用。  |  Osei-Sarfo, K., et al. 2012. Carcinogenesis. 33: 708-13. PMID: 22227036
  9. CDKN2B/P15甲基化与环境因素对胃癌易感性的共同影响  |  Zhou, H., et al. 2023. Dig Dis Sci. 68: 3009-3017. PMID: 36961670
  10. p15INK4B 是 TGF-beta 诱导的细胞周期停滞的潜在效应因子。  |  Hannon, GJ. and Beach, D. 1994. Nature. 371: 257-61. PMID: 8078588
  11. 一种细胞周期调节剂，可能与多种肿瘤类型的发生有关。  |  Kamb, A., et al. 1994. Science. 264: 436-40. PMID: 8153634
  12. p15（Ink4b）和 p27（Kip1）的亚细胞位置协调了它们与 cdk4 和 cdk2 的抑制性相互作用。  |  Reynisdóttir, I. and Massagué, J. 1997. Genes Dev. 11: 492-503. PMID: 9042862

订购信息

产品名称产品编号规格价格数量收藏夹

CDKN2B/p15 INK4B 抗体 (D-12)

sc-271791
200 µg/ml
$316.00

CDKN2B/p15 INK4B (D-12): m-IgG Fc BP-HRP 套装

sc-525823
200 µg Ab; 10 µg BP
$354.00

CDKN2B/p15 INK4B (D-12): m-IgG2a BP-HRP 套装

sc-546430
200 µg Ab; 10 µg BP
$354.00

CDKN2B/p15 INK4B 抗体 (D-12) AC

sc-271791 AC
500 µg/ml, 25% agarose
$416.00

CDKN2B/p15 INK4B 抗体 (D-12) HRP

sc-271791 HRP
200 µg/ml
$316.00

CDKN2B/p15 INK4B 抗体 (D-12) FITC

sc-271791 FITC
200 µg/ml
$330.00

CDKN2B/p15 INK4B 抗体 (D-12) PE

sc-271791 PE
200 µg/ml
$343.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 488

sc-271791 AF488
200 µg/ml
$357.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 546

sc-271791 AF546
200 µg/ml
$357.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 594

sc-271791 AF594
200 µg/ml
$357.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 647

sc-271791 AF647
200 µg/ml
$357.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 680

sc-271791 AF680
200 µg/ml
$357.00

CDKN2B/p15 INK4B 抗体 (D-12) Alexa Fluor® 790

sc-271791 AF790
200 µg/ml
$357.00

CDKN2B/p15 INK4B (D-12) 中和勝肽

sc-271791 P
100 µg/0.5 ml
$68.00

Buongiorno, potrei avere un'aliquota di prova?

Asked by: Anonymous
Grazie per la tua domanda. Contatta il nostro reparto di assistenza tecnica (o il tuo distributore locale) per ulteriori informazioni su come ricevere un campione GRATUITO da 10 µg
Answered by: Tech Support Europe
Date published: 2021-08-10

What is the positive control that you recommend for the p15 antibody? Thank you.

Asked by: AshE
Thank you for your question. There are many possible controls you can use for p15, such as A549 Cell Lysate (sc-2413), HeLa Whole Cell Lysate (sc-2200) or JAR Cell Lysate (sc-2276).
Answered by: Tech Service
Date published: 2020-01-02

What application is the blocking peptide sc-271791 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF analyisisThe antibodies were used in paraffin sections of humman brain tissue for IF analyisis, and 1:150 diluted the antibody ,the signal were very strong.
Date published: 2017-07-22
Rated 5 out of 5 by from expected resultThis is a small MW protein (15kda).We use the recommended conditions and WB protocol on santa Cruz website.We find 1:500 dilution ,and incubated antibody 1hour maybe better,we got the expected signal.
Date published: 2017-07-15
Rated 5 out of 5 by from Satisfied1/1000 dilution for my experiment, got a clear band.
Date published: 2017-05-12
Rated 5 out of 5 by from no problemWhen we use it in WB the results show very good, strong bands is dilution 1:500
Date published: 2017-04-19
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human p15 protein via Western Blot analysis. -SCBT QC
Date published: 2015-06-21
Rated 5 out of 5 by from Positive detection of endogenously expressedPositive detection of endogenously expressed p15 by WB using A549 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by from Published WB data with human stem cellsPublished WB data with human stem cells - SCBT Publication Review
Date published: 2014-07-07
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CDKN2B/p15 INK4B 抗体 (D-12) is rated 5.0 out of 5 by 7.
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