Date published: 2025-9-8
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p-Trk Antibody (E-6) - Western Blotting - Image 378993
p-Trk 抗体 (E-6): sc-8058. MEG-01 (A), Neuro-2A (B) 和 F9 (C) 全细胞裂解液中 Trk 磷酸化的 Western 印迹分析.
p-Trk 抗体 (E-6): sc-8058. MEG-01 (A), Neuro-2A (B) 和 F9 (C) 全细胞裂解液中 Trk 磷酸化的 Western 印迹分析.

p-Trk 抗体 (E-6): sc-8058

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寫評論提問

说明书
  • p-Trk抗体(E-6)是小鼠单克隆IgG1 κ, 在62篇文献中引用，规格为200 µg/ml
  • 抗-p-Trk 抗体 (E-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Trk A phosphorylated at Tyr 496 and correspondingly phosphorylated Trk B and Trk C
  • 抗p-Trk抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Trk (E-6): sc-8058的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 p-Trk 抗体 (E-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与p-Trk 抗体 (E-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Trk 抗体包括 Trk A, Trk B 和 Trk C

p-Trk抗体（E-6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中酪氨酸496位点磷酸化Trk以及相应磷酸化Trk B和Trk C，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学 和酶联免疫吸附测定（ELISA）。p-Trk (E-6)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物。Trk原癌基因编码一种跨膜蛋白酪氨酸激酶Trk gp140，这种蛋白对神经元的发育和功能至关重要，因为其表达主要局限于源自感觉脊髓和颅神经节的神经元。神经生长因子（NGF）对Trk A的磷酸化是一种快速且特异的过程，即使在皮摩尔浓度下也会发生，这凸显了NGF在神经元信号传导中的生理意义。这种磷酸化对于神经元的存活、分化与生长至关重要，使Trk A成为神经生长因子信号传导通路中的重要组成部分。相比之下，Trk B由脑源性神经营养因子（BDNF）磷酸化，而Trk C的磷酸化则由神经营养因子-3（NT-3）诱导，这表明这些神经营养因子在神经元健康与发育中发挥着不同的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Trk 抗体 (E-6) 参考文献:

  1. trk酪氨酸蛋白激酶介导神经生长因子和神经营养素-3的有丝分裂特性。  |  Cordon-Cardo, C., et al. 1991. Cell. 66: 173-83. PMID: 1649007
  2. trkB 酪氨酸蛋白激酶是脑源性神经营养因子和神经营养素-3 的受体。  |  Klein, R., et al. 1991. Cell. 66: 395-403. PMID: 1649702
  3. NGF 诱导的 trk 原癌基因产物的酪氨酸磷酸化和酪氨酸激酶活性。  |  Kaplan, DR., et al. 1991. Nature. 350: 158-60. PMID: 1706478
  4. 酪氨酸蛋白激酶受体 trk 家族。  |  Barbacid, M., et al. 1991. Biochim Biophys Acta. 1072: 115-27. PMID: 1751544
  5. trk 原癌基因编码神经生长因子的受体。  |  Klein, R., et al. 1991. Cell. 65: 189-97. PMID: 1849459
  6. 高亲和力 NGF 结合需要 trk 原癌基因和低亲和力 NGF 受体的共同表达。  |  Hempstead, BL., et al. 1991. Nature. 350: 678-83. PMID: 1850821
  7. 酪氨酸激酶受体基因 trkB 的表达仅限于小鼠胚胎和成年神经系统。  |  Klein, R., et al. 1990. Development. 109: 845-50. PMID: 2171894
  8. trkB，一种在小鼠神经发育过程中表达的新型酪氨酸蛋白激酶受体。  |  Klein, R., et al. 1989. EMBO J. 8: 3701-9. PMID: 2555172
  9. 临床癌症治疗中TRK蛋白的抑制。  |  Lange, AM. and Lo, HW. 2018. Cancers (Basel). 10: PMID: 29617282
  10. 培养的小脑颗粒神经元中 TrkC 受体的持续磷酸化可能是 NT-3 无法提高神经元存活率和激活 p21 Ras 的原因。  |  Zirrgiebel, U. and Lindholm, D. 1996. Neurochem Res. 21: 851-9. PMID: 8873090

订购信息

产品名称产品编号规格价格数量收藏夹

p-Trk 抗体 (E-6)

sc-8058
200 µg/ml
$316.00

p-Trk (E-6): m-IgG1 BP-HRP 套装

sc-531632
200 µg Ab; 20 µg BP
$354.00

p-Trk 抗体 (E-6) AC

sc-8058 AC
500 µg/ml, 25% agarose
$416.00

p-Trk 抗体 (E-6) HRP

sc-8058 HRP
200 µg/ml
$316.00

p-Trk 抗体 (E-6) FITC

sc-8058 FITC
200 µg/ml
$330.00

p-Trk 抗体 (E-6) PE

sc-8058 PE
200 µg/ml
$343.00

p-Trk 抗体 (E-6) Alexa Fluor® 488

sc-8058 AF488
200 µg/ml
$357.00

p-Trk 抗体 (E-6) Alexa Fluor® 546

sc-8058 AF546
200 µg/ml
$357.00

p-Trk 抗体 (E-6) Alexa Fluor® 594

sc-8058 AF594
200 µg/ml
$357.00

p-Trk 抗体 (E-6) Alexa Fluor® 647

sc-8058 AF647
200 µg/ml
$357.00

p-Trk 抗体 (E-6) Alexa Fluor® 680

sc-8058 AF680
200 µg/ml
$357.00

p-Trk 抗体 (E-6) Alexa Fluor® 790

sc-8058 AF790
200 µg/ml
$357.00

p-Trk (E-6) 中和勝肽

sc-8058 P
100 µg/0.5 ml
$68.00

As for the product p-Trk antibody (E-6): sc-8058, I would like to know if there is any information on the site (epitope) that the antibody binds to. Thank you for your support.

Asked by: Kazuo
Thank you for your question.sc-8058 p-Trk (E-6) antibody has not been epitope mapped . https://www.uniprot.org/uniprotkb/P04629/entry Thank you.
Answered by: Jiajun Z
Date published: 2024-12-25

What is the best secondary to use with this for WB?

Asked by: Anna99
Try our new Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-11

Can this mouse antibody be used for staining on mouse samples?

Asked by: Anna99
Yes. p-Trk Antibody (E-6) is available conjugated to HRP (sc-8058 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-8058 PE), fluorescein (sc-8058 FITC), Alexa Fluor® 488 (sc-8058 AF488) or Alexa Fluor® 647 (sc-8058 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 5 out of 5 by from Reliable antibody!It works in Western blot assays as well IFA assays!
Date published: 2022-07-20
Rated 1 out of 5 by from IF on whole mount sectionI used the sc-8058 antibody for IF on whole mount section of mouse small intestine, but the signal was very weak.
Date published: 2022-06-23
Rated 5 out of 5 by from p-Trk antibody for IHCI used this antibody for IHC on paraffin sections. It works very well with agood positive cytoplasmic signal, anyway I recommend also the use of the blocking peptide because it is possible also to see a background signal.
Date published: 2020-10-02
Rated 4 out of 5 by from 1:500 diluted for wb1:500 diluted for wb of rat protein ,the band is strong
Date published: 2019-03-28
Rated 2 out of 5 by from Worked for me in IHC but with backgroundIt worked in my IHC but with background. Maybe it is better to use the blocking peptide.
Date published: 2017-11-13
Rated 2 out of 5 by from IHC worked by with backgroundIt worked but with background. I think it is better to use the blocking peptide.
Date published: 2017-11-10
Rated 5 out of 5 by from Antibody works for WB with band detected at ~55kDaThank you for sending us a free sample of the mAb for pTrkA (E-6): sc-8058. The pTrkA antibody was used at 1:1000 and 1:300 dilutions in Western blot experiments with mouse eosinophil lysates. There were no detectable bands in the 140 kDa range. However, strong bands were detected at ~ 55 kDa. We will need to confirm whether this 55 kDa band is a cytosolic fragment of activated TrkA as indicated in a previous publication (Eur J Neurosci. 1999 Apr;11(4):1421-30). As such, we have ordered a blocking peptide for this mAb to confirm whether the bands at ~55 kDa are indeed fragments of TrkA.
Date published: 2017-11-06
Rated 5 out of 5 by from Good WBSignal is strong detected by WB at 1:500 ratio in K-562 cells, good result
Date published: 2017-05-20
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p-Trk 抗体 (E-6) is rated 4.3 out of 5 by 18.
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