p-ErbB2/HER2 Antibody (6G7): sc-81507

p-Neu Antibody (6G7) is a mouse monoclonal IgG1 antibody that detects p-Neu, specifically Tyr 1248 phosphorylated Neu, in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). p-Neu (6G7) antibody is available in a non-conjugated form, allowing for versatile applications in research. Neu protein, also known as v-ErbB-2 erythroblastic leukemia viral oncogene homolog 2, HER-2, and ErbB-2, plays a critical role in cell signaling pathways that regulate cell growth and differentiation. Neu structure resembles the human epidermal growth factor receptor, which mediates cellular responses to growth factors. Phosphorylation of Neu at Tyr 1248 facilitates interaction with key signaling proteins such as Shc, essential for propagating signals that can lead to oncogenic transformations. This interaction is crucial in various cancers, including breast, gastric, and esophageal cancers, as well as testicular germ cell tumors, highlighting Neu′s importance as a target for therapeutic interventions. Anti-p-Neu antibody (6G7) helps researchers gain insights into Neu phosphorylation status and implications in cancer biology, making this tool invaluable for advancing understanding of tumorigenesis.

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p-ErbB2/HER2 Antibody (6G7) References:

1. Neu differentiation factor stimulates phosphorylation and activation of the Sp1 transcription factor.  |  Alroy, I., et al. 1999. Mol Cell Biol. 19: 1961-72. PMID: 10022883
2. Activation (tyrosine phosphorylation) of ErbB-2 (HER-2/neu): a study of incidence and correlation with outcome in breast cancer.  |  Thor, AD., et al. 2000. J Clin Oncol. 18: 3230-9. PMID: 10986055
3. HER-2/neu induces p53 ubiquitination via Akt-mediated MDM2 phosphorylation.  |  Zhou, BP., et al. 2001. Nat Cell Biol. 3: 973-82. PMID: 11715018
4. Phosphorylation/cytoplasmic localization of p21Cip1/WAF1 is associated with HER2/neu overexpression and provides a novel combination predictor for poor prognosis in breast cancer patients.  |  Xia, W., et al. 2004. Clin Cancer Res. 10: 3815-24. PMID: 15173090
5. Expression, location, and interactions of ErbB2 and its intramembrane ligand Muc4 (sialomucin complex) in rat mammary gland during pregnancy.  |  Price-Schiavi, SA., et al. 2005. J Cell Physiol. 203: 44-53. PMID: 15499570
6. EGFR activity in HER-2 over-expressing metastatic breast cancer: evidence for simultaneous phosphorylation of Her-2/neu and EGFR.  |  Gschwantler-Kaulich, D., et al. 2005. Oncol Rep. 14: 305-11. PMID: 16012707
7. The transforming potential of the c-erbB-2 protein is regulated by its autophosphorylation at the carboxyl-terminal domain.  |  Akiyama, T., et al. 1991. Mol Cell Biol. 11: 833-42. PMID: 1671296
8. Analysis of protein-protein interactions involved in the activation of the Shc/Grb-2 pathway by the ErbB-2 kinase.  |  Ricci, A., et al. 1995. Oncogene. 11: 1519-29. PMID: 7478576
9. Tyrosine phosphorylation of Shc proteins and formation of Shc/Grb2 complex correlate to the transformation of NIH3T3 cells mediated by the point-mutation activated neu.  |  Xie, Y., et al. 1995. Oncogene. 10: 2409-13. PMID: 7784091
10. Structural determinants of the interaction between the erbB2 receptor and the Src homology 2 domain of Grb7.  |  Janes, PW., et al. 1997. J Biol Chem. 272: 8490-7. PMID: 9079677

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ErbB2/HER2 Antibody (6G7) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ErbB2/HER2 Antibody (6G7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ErbB2/HER2 Antibody (6G7) Specifications

Alternate Product Name: HER2

Product Clone Name: 6G7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: Tyr 1248

Epitope Target: Neu

Epitope Species: human

Product Reactivity/Detection: Tyr 1248 phosphorylated Neu

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for p-ErbB2/HER2 Antibody (6G7): N/A

The preferred antibody is Antibody (): sc-8.