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p-EGFR 抗体 (1H9): sc-57543

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说明书
  • p-EGFR 抗体 1H9 是小鼠单克隆 IgG1, 在2篇文献中引用,提供 50 µg/0.5 ml
  • 针对与EGFR的human来源的丝氨酸1047周围的氨基酸残基相对应的合成磷酸化肽
  • 推荐用于 mouse, rat, human 和 canine 来源的Ser 1047 phosphorylated EGFR WB, IP 和 IF检测
  • 看 EGFR (A-10): sc-373746,可查看有标记的 p-EGFR 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成p-EGFR 抗体 (1H9)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

表皮生长因子通过与指定为EGFR(表皮生长因子受体)的细胞表面糖蛋白的相互作用来调节它们对细胞生长的影响。EGF或TGFα与EGFR的结合激活了EGFR固有的酪氨酸特异性蛋白激酶活性。EGFR上的羧基末端酪氨酸残基Tyr 1092和Tyr 1173(在大鼠中指定为Tyr 1196)是发生自磷酸化的主要位点,这是由EGF结合引起的。一旦被激活,EGFR通过直接与人和小鼠中的Tyr 1092和Tyr 1110或大鼠中的Tyr 1109相互作用,并通过与Ras信号通路中的Tyr 1173间接相互作用,介导GRB2的磷酸酪氨酸结合(PTB)域的结合。EGFR的Tyr 1173也作为激酶底物起作用。Tyr 992、Tyr 1092和Tyr 1110的磷酸化是EGFR C端尾部构象变化所必需的。Tyr 1092、Tyr 1173和Tyr 1110也分别指定为Tyr 1068、Tyr 1197和Tyr 1086。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-EGFR 抗体 (1H9) 参考文献:

  1. 口腔鳞状细胞癌中表皮生长因子受体和磷酸化表皮生长因子受体的免疫组织化学表达。  |  Hiraishi, Y., et al. 2006. Pathol Oncol Res. 12: 87-91. PMID: 16799709
  2. 切除的非小细胞肺癌中的表皮生长因子受体(EGFR)突变和磷酸化EGFR表达。  |  McMillen, E., et al. 2010. Exp Lung Res. 36: 531-7. PMID: 20939760
  3. 胆囊癌中EGFR的核内导入:核内磷酸化EGFR上调iNOS表达,并具有独立的预后影响。  |  Li, CF., et al. 2012. Ann Surg Oncol. 19: 443-54. PMID: 21761100
  4. 磷酸化EGFR表达可预测野生型EGFR晚期NSCLC患者EGFR-TKI治疗的结果。  |  Wang, F., et al. 2012. J Exp Clin Cancer Res. 31: 65. PMID: 22901364
  5. EGFR和磷酸化EGFR与HPV和扁桃体癌临床结果的关系。  |  Romanitan, M., et al. 2013. Anticancer Res. 33: 1575-83. PMID: 23564800
  6. 食管鳞状细胞癌中磷酸化表皮生长因子受体和磷酸化Akt蛋白的表达及其预后。  |  Shan, ZZ., et al. 2017. Oncol Lett. 14: 2859-2863. PMID: 28927043
  7. miR-22-3p 通过 CBL/p-EGFR/p-STAT3/GAP43/p-GAP43 轴增强初级感觉神经元的内在再生能力。  |  Li, B., et al. 2020. J Cell Physiol. 235: 4605-4617. PMID: 31663116
  8. 鼻咽癌患者中表皮生长因子受体/磷酸化表皮生长因子受体的预后作用:荟萃分析。  |  Chen, X., et al. 2021. Front Oncol. 11: 697369. PMID: 34490092
  9. 表皮生长因子受体和磷酸化表皮生长因子受体在鼻咽癌中的预后价值:系统评价和荟萃分析。  |  Hong, X., et al. 2022. Medicine (Baltimore). 101: e28507. PMID: 35060503
  10. GLUT1通过结合和稳定磷酸化EGFR促进肺腺癌细胞增殖。  |  Zhou, Z., et al. 2024. Cell Commun Signal. 22: 303. PMID: 38831321

订购信息

产品名称产品编号规格价格数量收藏夹

p-EGFR 抗体 (1H9)

sc-57543
50 µg/0.5 ml
$322.00

What is the recommended starting dilution for Western blot for p-EGFR (1H9): sc-57543 monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by HF from Weak band with some background for WesternWeak band with some background for Western blot analysis using untreated and lambda protein phosphatase treated SK-OV-3 whole cell lysates. - SCBT QC
Date published: 2015-01-22
Rated 4 out of 5 by JT from Worked well with serum starved PMAWorked well with serum starved PMA-treated, Sorbit-treated, and Anisomycin-treated Hep G2 whole cell lysates. - SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by SS from Good specificity and signal strength for WB in AGood specificity and signal strength for WB in A-431 whole cell lysate. - SCBT QC
Date published: 2014-10-03
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p-EGFR 抗体 (1H9) is rated 4.0 out of 5 by 3.
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