Date published: 2026-10-26
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p-EGFR Antibody (15A2) - Western Blotting - Image 62921
p-EGFR 抗体 (15A2): sc-81488. 对未受刺激 (A), 受 EGF 刺激 (B) 和经普伐那酸处理 (C) 的 SKOV3 全细胞裂解液中的表皮生长因子受体磷酸化进行 Western 印迹分析.
p-EGFR Antibody (15A2) - Western Blotting - Image 62921
p-EGFR 抗体 (15A2): sc-81488. 对未受刺激 (A), 受 EGF 刺激 (B) 和经普伐那酸处理 (C) 的 SKOV3 全细胞裂解液中的表皮生长因子受体磷酸化进行 Western 印迹分析.
p-EGFR 抗体 (15A2): sc-81488. 对未受刺激 (A), 受 EGF 刺激 (B) 和经普伐那酸处理 (C) 的 SKOV3 全细胞裂解液中的表皮生长因子受体磷酸化进行 Western 印迹分析.

p-EGFR 抗体 (15A2): sc-81488

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说明书
  • p-EGFR 抗体 15A2 是小鼠单克隆 IgG1, 在13篇文献中引用，提供 50 µg/0.5 ml
  • 针对EGFR的human来源的酪氨酸1068周围的合成磷酸化肽
  • 推荐用于 mouse, rat, human 和 canine 来源的Tyr 1068 phosphorylated EGFR WB 和 IP检测
  • 看 EGFR (A-10): sc-373746，可查看有标记的 p-EGFR 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是p-EGFR Antibody (15A2) for WB applications. 的首选辅助检测试剂，这些试剂现与p-EGFR Antibody (15A2) 打包提供（请参阅下面的订购信息）。
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p-EGFR抗体（15A2）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠、人类和犬源性磷酸化表皮生长因子受体（p-EGFR）。p-EGFR单克隆抗体（15A2）以非结合型抗p-EGFR抗体形式提供。表皮生长因子受体（EGFR）通过与表皮生长因子的相互作用，在调节细胞生长、存活和分化方面发挥着至关重要的作用。当与EGF或TGFα等配体结合时，EGFR会发生构象变化，激活其内在的酪氨酸激酶活性，导致关键的酪氨酸残基（包括Tyr 1068、Tyr 1092和Tyr 1173）自磷酸化。这些磷酸化位点作为包含SH2或PTB结构域的信号蛋白的对接平台，确保下游效应子的精确招募和激活。自磷酸化也会导致EGFR细胞内结构域发生结构变化，从而增强与多种信号蛋白的相互作用，放大信号传导。这些磷酸化事件对于下游信号蛋白（如GRB2）的招募至关重要，GRB2可促进Ras信号通路的激活，而Ras信号通路是细胞增殖和存活的关键通路。p-EGFR能够介导这些信号级联，这凸显了其在各种生理过程中的重要性及其在 癌症进展，使其成为治疗干预和研究的宝贵靶标。抗-p-EGFR抗体（15A2）为研究人员提供了研究EGFR磷酸化及其在关键信号通路中作用的可靠工具，有助于加深对正常生理和肿瘤发生中功能的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-EGFR 抗体 (15A2) 参考文献:

  1. 表皮生长因子受体、EGFRvIII和磷酸化表皮生长因子受体在大肠癌中的共表达、预后意义和预测价值。  |  Cunningham, MP., et al. 2005. Int J Oncol. 27: 317-25. PMID: 16010411
  2. 表皮生长因子受体（EGFR）和磷酸化表皮生长因子受体在浸润性乳腺癌中的表达。  |  Magkou, C., et al. 2008. Breast Cancer Res. 10: R49. PMID: 18522728
  3. 胆囊癌中EGFR的核内导入：核内磷酸化EGFR上调iNOS表达，并具有独立的预后影响。  |  Li, CF., et al. 2012. Ann Surg Oncol. 19: 443-54. PMID: 21761100
  4. 层粘连蛋白5与PTEN、磷酸化EGFR和磷酸化Akt的组合定义了一组不同的分子亚群，表明非小细胞肺癌患者的预后较差。  |  An, SJ., et al. 2012. Exp Ther Med. 4: 226-230. PMID: 23139712
  5. 胞浆磷酸化EGFR可预测早期阴茎癌患者的复发：回顾性研究。  |  Di Lorenzo, G., et al. 2013. J Transl Med. 11: 161. PMID: 23819610
  6. IFIT1和IFIT3促进口腔鳞状细胞癌转移，并通过增强p-EGFR循环促进吉非替尼的抗肿瘤作用。  |  Pidugu, VK., et al. 2019. Oncogene. 38: 3232-3247. PMID: 30626937
  7. 磷酸化表皮生长因子受体（pEGFR T693）作为非功能性垂体腺瘤复发的新预测因子。  |  Rai, A., et al. 2021. Front Endocrinol (Lausanne). 12: 708111. PMID: 34295309
  8. 雌激素可减轻创伤后骨关节炎的发展，并降低雌性小鼠软骨中的p-EGFR水平。  |  Lu, Z., et al. 2022. BMC Musculoskelet Disord. 23: 685. PMID: 35854298
  9. 原位杂交（ISH）与免疫组织化学（IHC）联合检测肺癌组织中的EGFR RNA和磷酸化EGFR蛋白。  |  Oliver, A., et al. 2023. Methods Mol Biol. 2593: 221-232. PMID: 36513934

订购信息

产品名称产品编号规格价格数量收藏夹

p-EGFR 抗体 (15A2)

sc-81488
50 µg/0.5 ml
$322.00

p-EGFR (15A2): m-IgG Fc BP-HRP 套装

sc-539618
50 µg Ab; 10 µg BP
$361.00

p-EGFR (15A2): m-IgG1 BP-HRP 套装

sc-541604
50 µg Ab; 20 µg BP
$361.00

The data sheet says that Tyr1068 is also designated as Tyr1092. The protein reference NP_005219.2 shows me a Tyr at 1069 and at 1092. So which one does this antibody detect now?

Asked by: ToxVogel
Thank you for your question. These two phosphorylation sites are actually the same site, it's the numeration that varies for this protein. The site this antibody detects is in literature often referred to as Tyr 1068, but according to the swiss-prot accession#P00533, it is Tyr1092.
Answered by: Tech Service
Date published: 2019-11-20

I have purchase this antibody: "https://www.scbt.com/scbt/product/p-egfr-antibody-15a2"; but there are always two bands shown. one is near 170, the other is around 130. Is that right?

Asked by: Yiping111
Thank you for your question. Please contact our Technical Service Department for help with troubleshooting this antibody, and questions about the molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2021-11-17

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-02-14
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Rated 5 out of 5 by t888 from great antibodyclean band as expected. great antibody for western blot
Date published: 2020-12-01
Rated 5 out of 5 by Simon_Zhang from Free sample about p-EGFRIt's really good antibody, that I can detect the band over 130 kDa (marker).
Date published: 2018-03-16
Rated 5 out of 5 by Cweed from Great for WBThis antibody provide excellent detection of phospho-EGFR. It is ideal for WB and provides a reliably clear and specific signal. It has been used to publish multiple times.
Date published: 2017-02-08
Rated 4 out of 5 by HC from Great specificity for phosphorylated EGFRGreat specificity for phosphorylated EGFR as shown by EGF stimulation and pervanadate treatment in Western Blot -SCBT QC
Date published: 2014-01-05
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p-EGFR 抗体 (15A2) is rated 4.8 out of 5 by 4.
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