OSBP抗体 (H-5) 推荐用于 mouse, rat 和 human 来源的OSBP WB, IP, IF 和 ELISA检测

OSBP 抗体 (H-5): sc-515683

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说明书

    • OSBP抗体(H-5)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • 特异性抗原位于human物种的OSBP的C-terminus的氨基酸778-793之间
    • 推荐用于 mouse, rat 和 human 来源的OSBP WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

How much sample do you recommend loading on the gel for Western blot detection using OSBP (H-5): sc-515683?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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