Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NUDT21 CRISPR/Cas9 KO Plasmid (h): sc-402082
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NUDT21 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NUDT21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NUDT21 Antibody (2203C3): sc-81109
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUDT21 CRISPR/Cas9 KO Plasmid (h) | sc-402082 | 20 µg | $397.00 |
Overview
NUDT21 encodes a core subunit of the cleavage factor Im (CFIm) complex that recognizes UGUA motifs and promotes 3′ end processing and polyadenylation of pre-mRNAs. By influencing alternative polyadenylation site choice, NUDT21 helps control 3′ UTR length, mRNA stability, and translational output, thereby shaping gene expression programs linked to proliferation, differentiation, and stress responses. Altered NUDT21 activity is associated with widespread shifts in alternative polyadenylation that can remodel regulatory networks, including microRNA-mediated control and RNA-binding protein interactions. Dysregulation of these RNA processing pathways has been implicated in diverse disease-relevant contexts, including cancer-associated transcriptome remodeling and neurodevelopmental phenotypes.
NUDT21 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NUDT21 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NUDT21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NUDT21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NUDT21 protein expression.
This CRISPR knockout system enables efficient generation of NUDT21-deficient cell models for investigation of NUDT21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NUDT21 exon(s) critical for NUDT21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NUDT21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NUDT21 CRISPR/Cas9 KO Plasmid (h) and NUDT21 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NUDT21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NUDT21 HDR Plasmid (h) and NUDT21 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NUDT21 homology arms to support homology-directed repair at defined NUDT21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.