Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

NRAMP 1 抗体 (E-2): sc-398077

4.5(2)
寫評論提問

说明书
  • NRAMP 1 抗体 E-2 是小鼠单克隆 IgG3 κ,NRAMP 1抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的NRAMP 1的内部区域内的氨基酸68-99之间
  • 推荐用于 mouse, rat 和 human 来源的NRAMP 1 and NRAMP 2 WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine and bovine
  • 关于如何获取NRAMP 1 (E-2): sc-398077免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是NRAMP 1 Antibody (E-2) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与NRAMP 1 Antibody (E-2) 搭配使用(请参阅下面的订购信息)。

快捷链接

関連項目

vNRAMP 1抗体(E-2)是一种IgG3 κ小鼠单克隆NRAMP 1抗体(也称为NRAMP 1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的NRAMP 1蛋白和NRAMP 2蛋白。NRAMP 1抗体(E-2)为非偶联型抗NRAMP 1抗体。天然抗性相关巨噬细胞蛋白(NRAMP)属于高度保守的整体膜蛋白超家族。NRAMP 1是一种位于吞噬体膜上的细胞内巨噬细胞蛋白,在吞噬体膜上作为Fe2+、Zn2+和Mn2+的二价阳离子转运蛋白发挥作用。NRAMP 1是一种依赖于pH值的反向转运蛋白,它可将金属离子逆质子梯度运入或运出吞噬体。在人类中,NRAMP 1基因的多态性与对结核分枝杆菌和麻风的易感性有关。NRAMP 2是另一种二价阳离子转运蛋白,普遍表达为两种剪接变体,这两种剪接变体可通过铁反应元件的存在(异构体1)或缺失(异构体2)来区分。在小肠的十二指肠中,膳食铁可调节位于刷状缘的NRAMP 2表达。小鼠和大鼠NRAMP 2基因的突变会导致严重贫血。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NRAMP 1 抗体 (E-2) 参考文献:

  1. Nramp2 铁转运体在肠刷状边界的细胞和亚细胞定位以及膳食铁的调控。  |  Canonne-Hergaux, F., et al. 1999. Blood. 93: 4406-17. PMID: 10361139
  2. 自然抗性相关巨噬细胞蛋白 1 是一种 H+/二价阳离子反转运体。  |  Goswami, T., et al. 2001. Biochem J. 354: 511-9. PMID: 11237855
  3. 从 Bcg/Lsh/Ity 到 Nramp1:三十年的探索与研究。  |  Buschman, E. and Skamene, E. 2001. Drug Metab Dispos. 29: 471-3. PMID: 11259334
  4. NRAMP1 基因与几内亚-科纳克里结核病易感性之间的等全基因关联。  |  Cervino, AC., et al. 2000. Ann Hum Genet. 64: 507-12. PMID: 11281214
  5. 克隆和鉴定染色体 12q13 上的第二个人类 NRAMP 基因。  |  Vidal, S., et al. 1995. Mamm Genome. 6: 224-30. PMID: 7613023
  6. 人类天然抵抗力相关巨噬细胞蛋白:cDNA 克隆, 染色体图谱, 基因组组织和组织特异性表达。  |  Cellier, M., et al. 1994. J Exp Med. 180: 1741-52. PMID: 7964458
  7. 麻风病的易感性与人类 NRAMP1 基因有关。  |  Abel, L., et al. 1998. J Infect Dis. 177: 133-45. PMID: 9419180
  8. 西非人 NRAMP1 基因变异与结核病易感性。  |  Bellamy, R., et al. 1998. N Engl J Med. 338: 640-4. PMID: 9486992
  9. 人类 Nramp2 基因:基因结构, 替代剪接, 启动子区域和多态性的特征。  |  Lee, PL., et al. 1998. Blood Cells Mol Dis. 24: 199-215. PMID: 9642100
  10. G185R 突变会破坏铁转运体 Nramp2 的功能。  |  Su, MA., et al. 1998. Blood. 92: 2157-63. PMID: 9731075

订购信息

产品名称产品编号规格价格数量收藏夹

NRAMP 1 抗体 (E-2)

sc-398077
200 µg/ml
$316.00

NRAMP 1 (E-2): m-IgG3 BP-HRP 套装

sc-550601
200 µg Ab; 40 µg BP
$354.00

NRAMP 1 (E-2) 中和勝肽

sc-398077 P
100 µg/0.5 ml
$68.00

As there any information about the conc for IF? AYEN already used this for IF but no details about the conc?

Asked by: Rafiq
Thank you for your question. We recommend a starting dilution of 1:50 for this antibody in IF applications. You can find this information on the product datasheet and in the IF protocol: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: Tech Service
Date published: 2019-12-17

What application is the blocking peptide sc-398077 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_398077, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from NRMAP1 in the cortex of mouse brainI asked for a sample of NRAMP1 Antibody (E-2) for checking if NRAMP1 expressed in astrocytes. Although this protein was not detected in the GFAP+ astrocytes (maybe in microglia and vessels), the staining worked well.
Date published: 2018-04-03
Rated 5 out of 5 by from Very good Western blot data of NRAMP 1 expressionVery good Western blot data of NRAMP 1 expression in U-937 whole cell lysate. -SCBT QC
Date published: 2013-01-13
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_398077, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
NRAMP 1 抗体 (E-2) is rated 4.5 out of 5 by 2.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_398077, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT