Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Notch1 Double Nickase Plasmid (m): sc-421930-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Notch1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Notch1 Double Nickase Plasmid (m) and Notch1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Notch1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Notch1 Antibody (A-8): sc-376403
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Notch1 Double Nickase Plasmid (m) | sc-421930-NIC | 20 µg | $410.00 | |||
Notch1 Double Nickase Plasmid (m2) | sc-421930-NIC-2 | 20 µg | $410.00 |
Mouse Notch1 encodes a single-pass transmembrane receptor that mediates canonical Notch signaling to control cell fate decisions, stem and progenitor maintenance, and differentiation programs during development and tissue homeostasis. Ligand engagement triggers proteolytic processing and release of the Notch intracellular domain, which translocates to the nucleus and cooperates with RBPJ/CSL and coactivators such as MAML to regulate transcriptional networks including HES and HEY family targets. Notch1 integrates with pathways governing proliferation, apoptosis, and lineage commitment, and its dysregulation is associated with altered immune cell development and tumorigenic processes in multiple tissues. In mouse models, Notch1 perturbation is widely used to interrogate developmental phenotypes, hematopoietic regulation, and microenvironmental cues that shape tissue organization.
Notch1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Notch1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Notch1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Notch1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Notch1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.