Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)

NOD2 抗体 (2D9): sc-56168

4.4(10)
寫評論提問

说明书
  • NOD2 抗体 2D9 是小鼠单克隆 IgG1 κ，NOD2抗体, 在19篇文献中引用，规格为200 µg/ml
  • 免疫human物种的NOD2的氨基酸28-301
  • NOD2 抗体 (2D9) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 NOD2
  • 抗NOD2抗体（2D9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NOD2 (2D9): sc-56168的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 NOD2 抗体 (2D9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NOD2 抗体 (2D9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Nod 抗体 包括 NOD1, NOD2, NOD3, NOD9, Nop2p, Nop56p和Nop5p

NOD2抗体（2D9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人源的NOD2蛋白。NOD2 (2D9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。NOD2蛋白通过识别细菌成分并激活信号通路来产生促炎细胞因子，在免疫反应中起着至关重要的作用。这一功能对于宿主抵抗感染至关重要，特别是在肠道中，NOD2有助于维持肠道稳态并预防炎症性疾病，如克罗恩病。从结构上看，NOD2由两个N端半胱氨酸蛋白酶募集结构域、一个中心核苷酸结合结构域和多个C端富含亮氨酸的重复结构域组成，这些结构域有助于与信号级联中的其他蛋白相互作用。NOD2主要在单核细胞中表达，NFκB对细菌脂多糖的反应激活强调了NOD2在先天免疫中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NOD2 抗体 (2D9) 参考文献:

  1. 人类 CARD4 蛋白是一种新型 CED-4/Apaf-1 细胞死亡家族成员，可激活 NF-kappaB。  |  Bertin, J., et al. 1999. J Biol Chem. 274: 12955-8. PMID: 10224040
  2. Nod1，一种类似 Apaf-1 的 Caspase-9 和核因子-kappaB 激活剂。  |  Inohara, N., et al. 1999. J Biol Chem. 274: 14560-7. PMID: 10329646
  3. NF-kappa B 在 Nod1/RICK 和 RIP 信号通路中激活的诱导接近模型。  |  Inohara, N., et al. 2000. J Biol Chem. 275: 27823-31. PMID: 10880512
  4. 哺乳动物 Ced-4 细胞凋亡蛋白家族新成员 DEFCAP-L 和 -S 的两种同工型的分子克隆和表征。  |  Hlaing, T., et al. 2001. J Biol Chem. 276: 9230-8. PMID: 11076957
  5. Nod2 是一种 Nod1/Apaf-1 家族成员，仅限于单核细胞，能激活 NF-kappaB。  |  Ogura, Y., et al. 2001. J Biol Chem. 276: 4812-8. PMID: 11087742
  6. NOD1和NOD2信号将ER应激与炎症联系起来。  |  Keestra-Gounder, AM., et al. 2016. Nature. 532: 394-7. PMID: 27007849
  7. 细菌感应需要NOD1和NOD2的棕榈酰化。  |  Lu, Y., et al. 2019. Science. 366: 460-467. PMID: 31649195
  8. NOD1和NOD2在炎症和感染性疾病中的作用。  |  Trindade, BC. and Chen, GY. 2020. Immunol Rev. 297: 139-161. PMID: 32677123
  9. 棕榈酰化限制了SQSTM1/p62介导的NOD2自噬降解，从而调节炎症。  |  Zhou, L., et al. 2022. Cell Death Differ. 29: 1541-1551. PMID: 35066577
  10. 微生物介导的肠道 NOD2 刺激可改善营养不良婴儿小鼠的线性生长。  |  Schwarzer, M., et al. 2023. Science. 379: 826-833. PMID: 36821686
  11. NOD2通过重新编程激活滑膜巨噬细胞来减轻骨关节炎。  |  Li, C., et al. 2023. Arthritis Res Ther. 25: 249. PMID: 38124066
  12. 人类 Nod1 能增强对细菌脂多糖的反应能力  |  Inohara, N., et al. 2001. J Biol Chem. 276: 2551-4. PMID: 11058605

订购信息

产品名称产品编号规格价格数量收藏夹

NOD2 抗体 (2D9)

sc-56168
200 µg/ml
$316.00

NOD2 (2D9): m-IgG Fc BP-HRP 套装

sc-526877
200 µg Ab; 10 µg BP
$354.00

NOD2 (2D9): m-IgG1 BP-HRP 套装

sc-532250
200 µg Ab; 20 µg BP
$354.00

NOD2 抗体 (2D9) AC

sc-56168 AC
500 µg/ml, 25% agarose
$416.00

NOD2 抗体 (2D9) HRP

sc-56168 HRP
200 µg/ml
$316.00

NOD2 抗体 (2D9) FITC

sc-56168 FITC
200 µg/ml
$330.00

NOD2 抗体 (2D9) PE

sc-56168 PE
200 µg/ml
$343.00

NOD2 抗体 (2D9) Alexa Fluor® 488

sc-56168 AF488
200 µg/ml
$357.00

NOD2 抗体 (2D9) Alexa Fluor® 546

sc-56168 AF546
200 µg/ml
$357.00

NOD2 抗体 (2D9) Alexa Fluor® 594

sc-56168 AF594
200 µg/ml
$357.00

NOD2 抗体 (2D9) Alexa Fluor® 647

sc-56168 AF647
200 µg/ml
$357.00

NOD2 抗体 (2D9) Alexa Fluor® 680

sc-56168 AF680
200 µg/ml
$357.00

NOD2 抗体 (2D9) Alexa Fluor® 790

sc-56168 AF790
200 µg/ml
$357.00

Hi i just got your Santa cruz antibody NOD-2 sc-56168 for my westernblot, but I cant find the formula on how to dilute these antibody. BSA or skim milk? TBST or PBS? can it be frozen after dilution? Best Regards

Asked by: dfjk2d
Thank you for your question. The primary antibody can be diluted with blocking buffer, which is 5% milk in TBST. It can't be frozen after dilution.
Answered by: Technical Service SH
Date published: 2022-08-17

what positive control can be used with the NOD2 Antibody (2D9): sc-56168 for western blot?

Asked by: Henda
Thank you for your question. According to the following product citation using this NOD2 antibody in Western blot, HT-29 and HCT-116 whole cell lysates are both suitable positive controls for NOD2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151099/
Answered by: Technical Support
Date published: 2019-03-04

Can NOD2 (2D9): sc-56168 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 15
Date published: 2017-01-24

Does the NOD2 Antibody (2D9) have any reactivity with bovine samples?

Asked by: Trav11
To check additional species reactivity, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-19

Is it possible to use the NOD2 Antibody (2D9) for immunohistochemistry in mouse tissue samples?

Asked by: Trav11
Yes, it is possible. In this case, we recommend using our mouse monoclonal primary antibodies that are directly conjugated. Currently, the NOD2 Antibody (2D9): sc-56168 is available directly conjugated to HRP (sc-56168 HRP), phycoerythrin (sc-56168 PE), fluorescein (sc-56168 FITC), Alexa Fluor® 488 (sc-56168 AF488) or Alexa Fluor® 647 (sc-56168 AF647).
Answered by: Tech Service 8
Date published: 2016-12-19
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_56168, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 80ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from NOD2 抗体 (2D9)效果不错采用NOD2 抗体 (2D9)进行NOD2 蛋白检测，检测早期妊娠对绵羊肝脏表达NOD2 蛋白的影响，条带明显。
Date published: 2023-10-21
Rated 5 out of 5 by from It is pretty goodThis antibody (NOD2 Antibody (2D9)) worked well for WB. Samples were ovine thymuses at dilution ratio 1:1000.
Date published: 2023-03-17
Rated 1 out of 5 by from Worst antibody I have ever usedI bought this antibody recently and it does not work at all. I see that this antibody has received great reviews for western blotting in the past but something has obviously changed. Not only does it not detect the target to give a band at the proper size in any cell line that we have tried, it appears to make the background way worse than if you were to not have any primary antibody at all. If you have an older Lot of this antibody I would treat it very well because it would seem that Santa Cruz quality control has fallen off a cliff lately.
Date published: 2020-12-06
Rated 5 out of 5 by from Excellent Ab, it works everytime, clean and clear.The AB works every time, you get clean and clear bands and it detects even endogenous NOD2.
Date published: 2018-01-17
Rated 5 out of 5 by from good antibodyI bought the NOD2 antibody, works good in western, strong signal for WB.
Date published: 2017-04-25
Rated 3 out of 5 by from WBWe have not got any signal in human samples by IHC-P or WB
Date published: 2017-04-19
Rated 5 out of 5 by from Popular choice for NOD2sc-56168: NOD2 (2D9) is a very popular choice published in a variety of research applications including ELISA, WB, IHC and Flow cytometry.
Date published: 2016-12-29
Rated 5 out of 5 by from good antibodygood antibody in WB lysis of BMDM cells...........
Date published: 2016-12-02
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_56168, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
NOD2 抗体 (2D9) is rated 4.4 out of 5 by 10.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_56168, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。