Date published: 2025-9-6
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NMDAε2 Antibody (A-8) - Western Blotting - Image 378918
NMDAε2 抗体 (A-8): sc-365597. 大鼠脑 (A) 和小鼠脑 (B) 组织提取物中 NMDAε2 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
NMDAε2 抗体 (A-8): sc-365597. 大鼠脑 (A) 和小鼠脑 (B) 组织提取物中 NMDAε2 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

NMDAε2 抗体 (A-8): sc-365597

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说明书
  • NMDA epsilon 2 抗体 A-8 是小鼠单克隆 IgG2a κ，NMDAε2抗体, 在26篇文献中引用，规格为200 µg/ml
  • 与human的NMDAε2的细胞外结构域内的氨基酸27-76杂交
  • NMDA epsilon 2 抗体 (A-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 glutamate (NMDA) receptor subtype epsilon 2
  • 抗NMDA epsilon 2抗体（A-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NMDAε2 (A-8): sc-365597的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 NMDAε2 抗体 (A-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NMDAε2 抗体 (A-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 NMDA 抗体 包括 NMDA ε 1, NMDA ε 2, NMDA ε 4和NMDA ζ 1

NMDAε2抗体（A-8）是一种IgG2aκ小鼠单克隆NMDAε2抗体（也称为谷氨酸离子型受体NMDA型亚基2B抗体、GRIN2B抗体、谷氨酸[NMDA]受体亚基ε-2抗体、谷氨酸门控离子通道蛋白家族成员2B抗体、NR2B抗体、N-甲基-D-天冬氨酸（NMDA）受体亚基抗体或NMDAR2B抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的NMDAε2蛋白。NMDAε2抗体（A-8）既有非偶联抗NMDAε2抗体形式，也有多种偶联形式的抗NMDAε2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。谷氨酸受体介导大脑中大多数兴奋性神经传递，并在神经可塑性、神经发育和神经退行性变中发挥重要作用。促离子型谷氨酸受体分为NMDA受体和卡因酸/AMPA受体，两者都含有谷氨酸门控的阳离子特异性离子通道。卡因酸/AMPA受体与NMDA受体在许多突触中共定位，由七个结构相关的亚基组成，分别命名为GluR-1至-7。卡因酸/AMPA受体主要负责谷氨酸的快速兴奋性神经传递，而NMDA受体则表现出Ca2+离子的慢动力学和对Ca2+离子的高渗透性。NMDA受体由五个亚基组成：ε1、2、3、4和一个ζ亚基。ζ亚基在整个脑干中表达，而四个ε亚基则显示有限的分布。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NMDAε2 抗体 (A-8) 参考文献:

  1. 大鼠视觉皮层切片受到局灶性刺激时星状细胞上的单位 EPSC 的快速和慢速成分。  |  Stern, P., et al. 1992. J Physiol. 449: 247-78. PMID: 1326045
  2. 谷氨酸受体的分子多样性及其对大脑功能的影响。  |  Nakanishi, S. 1992. Science. 258: 597-603. PMID: 1329206
  3. 谷氨酸神经毒性在缺氧缺血性神经元死亡中的作用  |  Choi, DW. and Rothman, SM. 1990. Annu Rev Neurosci. 13: 171-82. PMID: 1970230
  4. 神经发育障碍中的人类 GRIN2B 变异。  |  Hu, C., et al. 2016. J Pharmacol Sci. 132: 115-121. PMID: 27818011
  5. GRIN2B脑病：关于表型、变异集群、功能性后果和治疗方面的新发现。  |  Platzer, K., et al. 2017. J Med Genet. 54: 460-470. PMID: 28377535
  6. GRIN2A和GRIN2B变体在神经系统疾病中的不同作用。  |  Myers, SJ., et al. 2019. F1000Res. 8: PMID: 31807283
  7. 芬太尼通过谷氨酸受体基因 Grin2b 的超甲基化诱导小鼠出现类似自闭症的行为。  |  Sheng, Z., et al. 2022. Br J Anaesth. 129: 544-554. PMID: 35697546
  8. GRIN1、GRIN2A 和 GRIN2B 基因中的疾病相关变异：洞察 NMDA 受体的结构、功能和病理生理学。  |  Korinek, M., et al. 2024. Physiol Res. 73: S413-S434. PMID: 38836461
  9. 脑干中五种 NMDA 受体通道亚基 mRNA 的不同分布。  |  Watanabe, M., et al. 1994. J Comp Neurol. 343: 520-31. PMID: 7518475
  10. 克隆谷氨酸受体  |  Hollmann, M. and Heinemann, S. 1994. Annu Rev Neurosci. 17: 31-108. PMID: 8210177
  11. 记忆的突触模型：海马体的长期电位。  |  Bliss, TV. and Collingridge, GL. 1993. Nature. 361: 31-9. PMID: 8421494
  12. 大鼠 GluR7 和羧基末端剪接变体 GluR7b 是对谷氨酸敏感性较低的功能性 kainate 受体亚基。  |  Schiffer, HH., et al. 1997. Neuron. 19: 1141-6. PMID: 9390526

订购信息

产品名称产品编号规格价格数量收藏夹

NMDAε2 抗体 (A-8)

sc-365597
200 µg/ml
$316.00

NMDAε2 (A-8): m-IgG Fc BP-HRP 套装

sc-529402
200 µg Ab; 10 µg BP
$354.00

NMDAε2 (A-8): m-IgGκ BP-HRP 套装

sc-522283
200 µg Ab, 40 µg BP
$354.00

NMDAε2 抗体 (A-8) AC

sc-365597 AC
500 µg/ml, 25% agarose
$416.00

NMDAε2 抗体 (A-8) HRP

sc-365597 HRP
200 µg/ml
$316.00

NMDAε2 抗体 (A-8) FITC

sc-365597 FITC
200 µg/ml
$330.00

NMDAε2 抗体 (A-8) PE

sc-365597 PE
200 µg/ml
$343.00

NMDAε2 抗体 (A-8) Alexa Fluor® 488

sc-365597 AF488
200 µg/ml
$357.00

NMDAε2 抗体 (A-8) Alexa Fluor® 546

sc-365597 AF546
200 µg/ml
$357.00

NMDAε2 抗体 (A-8) Alexa Fluor® 594

sc-365597 AF594
200 µg/ml
$357.00

NMDAε2 抗体 (A-8) Alexa Fluor® 647

sc-365597 AF647
200 µg/ml
$357.00

NMDAε2 抗体 (A-8) Alexa Fluor® 680

sc-365597 AF680
200 µg/ml
$357.00

NMDAε2 抗体 (A-8) Alexa Fluor® 790

sc-365597 AF790
200 µg/ml
$357.00

你好跑wb有哪些条件可以说明一下吗，跑了很多回不出条带

Asked by: 武晓彬斌
感谢您的提问。您可以参考我们的WB实验流程：https://www.scbt.com/zh/resources/protocols/western-immuno-blotting；如有其他问题，您可以拨打电话：021-60936350，发送邮件到asia@scbio.cn，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。谢谢。 Thank you for your question. You can refer to our WB experimental process: https://www.scbt.com/zh/resources/protocols/western-immuno-blotting; If you have any further questions, you can call 021-60936350, email us at asia@scbio.cn, or contact our Asia Technical Support team during our live chat on our website for more information. Thank you
Answered by: Jiajun Z
Date published: 2023-04-23

Can NMDAε2 (A-8): sc-365597 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, NMDAε2 (A-8): sc-365597 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

I would like to purchase GluN1 and GluN2C antibodies for WB in rat, but I didn't find them in your catalogue. Can you help me with this or can you give me an alternative? Thank you in advance.

Asked by: Marcia1
Thank you for your question. We do not currently offer antibodies to these protein targets at this time. We are working to add new products to our catalogue every month so please check back frequently!
Answered by: Technical Support
Date published: 2017-01-10
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Rated 3 out of 5 by from Resultado no reproducibleMe otorgaron un muestra de este anticuerpo y desafortuandamente no pude reproducir el resultado
Date published: 2024-03-20
Rated 4 out of 5 by from Buon funzionamento!Ho testato questo anticorpo su campioni di topo ottenendo un buon segnale all'altezza prevista.
Date published: 2021-02-22
Rated 5 out of 5 by from Great antibodyWorks great. Strong signal, low background. You can start with 1:1000.
Date published: 2017-07-27
Rated 3 out of 5 by from hard workwe ttied hard to get a band ,but we failed ,we tried different concentration，but we got nothing
Date published: 2017-03-02
Rated 5 out of 5 by from Good antibody for WBGet srong signal for NR2B in the predicted place, good for WB.
Date published: 2017-02-23
Rated 5 out of 5 by from I will introduce the with partners.The analysis results of Western blot were very strong and clear.
Date published: 2017-02-13
Rated 5 out of 5 by from Powerful cell antibodiesStrong cytoplasmic staining paraffin embedding human breast samples, will recommend to the partner
Date published: 2017-02-09
Rated 5 out of 5 by from Excellent antibodyNMDAε2 antibody worked well in the mouse samples. We got obtained specific band, good specificity and strong signal.
Date published: 2017-02-09
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NMDAε2 抗体 (A-8) is rated 4.6 out of 5 by 12.
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