Date published: 2025-9-6

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NKCC1 抗体 (F-4): sc-514858

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说明书
  • NKCC1抗体(F-4)是小鼠单克隆IgG2a κ, 在2篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的NKCC1的N-terminus附近的氨基酸78-99之间
  • NKCC1 抗体 (F-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 NKCC1
  • 抗NKCC1抗体(F-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NKCC1 (F-4): sc-514858免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 NKCC1 抗体 (F-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NKCC1 抗体 (F-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

NKCC1抗体(F-4)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类NKCC1。NKCC1 (F-4) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。NKCC1 在氯离子跨上皮组织进行跨细胞移动中起着至关重要的作用,对于维持体内体液平衡和电解质稳态至关重要。NKCC1主要在肌肉细胞、神经元和红细胞中表达,促进分泌上皮基底膜中氯离子的主动分泌。编码人类NKCC1的基因位于5q23.3染色体上,小鼠NKCC1的缺失与耳聋和平衡障碍有关,这凸显了NKCC1在生理功能中的重要性。NKCC1是钠-钾-氯共转运体家族中的一部分,该家族还包括主要在肾脏中表达并参与氯化钠重吸收的NKCC2,以及对噻嗪类利尿剂敏感并在肾脏钠重吸收中发挥重要作用的NCCT。了解NKCC1的功能和调节对于制定电解质失衡和体液潴留相关疾病的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NKCC1 抗体 (F-4) 参考文献:

  1. NKCC1 与高血压:在调节血管平滑肌收缩和肌张力中的作用。  |  Orlov, SN., et al. 2012. Ann Med. 44 Suppl 1: S111-8. PMID: 22713139
  2. NKCC1 氯化物输入体拮抗剂可减轻多种神经和精神疾病。  |  Ben-Ari, Y. 2017. Trends Neurosci. 40: 536-554. PMID: 28818303
  3. NKCC1 和 KCC2 在癫痫中的作用:从表达到功能  |  Liu, R., et al. 2019. Front Neurol. 10: 1407. PMID: 32010056
  4. NKCC1:大脑发育中难以捉摸的分子靶标:现有数据的意义。  |  Virtanen, MA., et al. 2020. Cells. 9: PMID: 33291778
  5. 脉络丛 NKCC1 在小鼠出生后早期发育过程中介导脑脊液清除。  |  Xu, H., et al. 2021. Nat Commun. 12: 447. PMID: 33469018
  6. NKCC1 和 KCC 转运体的高分辨率视图和转运机制。  |  Chew, TA., et al. 2021. J Mol Biol. 433: 167056. PMID: 34022207
  7. SPAK-NKCC1 信号级联在中风后脉络丛血-脑脊液屏障损伤中的作用  |  Wang, J., et al. 2022. J Neuroinflammation. 19: 91. PMID: 35413993
  8. 皮质海马发育过程中神经元和非神经元细胞中 NKCC1 的表达模式  |  Kurki, SN., et al. 2023. Cereb Cortex. 33: 5906-5923. PMID: 36573432
  9. KCC2 和 NKCC1 在脊髓损伤中的作用:从生理学到病理学  |  Talifu, Z., et al. 2022. Front Physiol. 13: 1045520. PMID: 36589461
  10. 作为神经和神经精神疾病治疗靶点的阳离子-氯化物共转运体 KCC2 和 NKCC1。  |  Lam, P., et al. 2023. Molecules. 28: PMID: 36771011

订购信息

产品名称产品编号规格价格数量收藏夹

NKCC1 抗体 (F-4)

sc-514858
200 µg/ml
$316.00

NKCC1 (F-4): m-IgG Fc BP-HRP 套装

sc-531235
200 µg Ab; 10 µg BP
$354.00

NKCC1 (F-4): m-IgGκ BP-HRP 套装

sc-524865
200 µg Ab, 40 µg BP
$354.00

NKCC1 (F-4): m-IgG2a BP-HRP 套装

sc-547736
200 µg Ab; 10 µg BP
$354.00

NKCC1 抗体 (F-4) AC

sc-514858 AC
500 µg/ml, 25% agarose
$416.00

NKCC1 抗体 (F-4) HRP

sc-514858 HRP
200 µg/ml
$316.00

NKCC1 抗体 (F-4) FITC

sc-514858 FITC
200 µg/ml
$330.00

NKCC1 抗体 (F-4) PE

sc-514858 PE
200 µg/ml
$343.00

NKCC1 抗体 (F-4) Alexa Fluor® 488

sc-514858 AF488
200 µg/ml
$357.00

NKCC1 抗体 (F-4) Alexa Fluor® 546

sc-514858 AF546
200 µg/ml
$357.00

NKCC1 抗体 (F-4) Alexa Fluor® 594

sc-514858 AF594
200 µg/ml
$357.00

NKCC1 抗体 (F-4) Alexa Fluor® 647

sc-514858 AF647
200 µg/ml
$357.00

NKCC1 抗体 (F-4) Alexa Fluor® 680

sc-514858 AF680
200 µg/ml
$357.00

NKCC1 抗体 (F-4) Alexa Fluor® 790

sc-514858 AF790
200 µg/ml
$357.00

NKCC1 (F-4) 中和勝肽

sc-514858 P
100 µg/0.5 ml
$68.00

Hi Blake, the catalog number for the N-16 antibody was sc-21545. So I am hoping to confirm whether the F4 antibody (catalog number sc-514858) was raised against the same epitope. Thanks.

Asked by: C Thorne
Thank you for contacting Santa Cruz Biotechnology . The epitope range for sc-21545 was 79-94. The epitope range for sc-514858 is 78-99.
Answered by: BlakeJ
Date published: 2023-05-03

Hello, Can you confirm if this is raised against the same epitope as the previous antibody (NKCC1-N16) was raised against? Also, is the blocking peptide sc-514858 P still available for purchase for our use as a specificity control?

Asked by: C Thorne
Thank you for your question. To check the epitope please provide the catalog number for the previous antibody . The blocking peptide is available for purchase .
Answered by: BlakeJ
Date published: 2023-05-01

What application is the blocking peptide sc-514858 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Doesn't workI got this sample in replacement of NKCC1 (N-16) for immunofluorescence staining. N-16 is great but this one is not. There was no signal under the same condition, or even at a higher concentration (1:50 dilution).
Date published: 2017-01-31
Rated 1 out of 5 by from Not working for immunofluorescence stainingThe NKCC1 (N16) give us perfect staining. However, using the same condition, this one give up nothing for the immunofluorescence staining.
Date published: 2016-11-11
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NKCC1 抗体 (F-4) is rated 1.0 out of 5 by 2.
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