Date published: 2025-9-6
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NG2 Antibody (LHM 2) - Western Blotting - Image 15830
NG2 抗体 (LHM 2): sc-53389. 对 SK-MEL-24 全细胞裂解液中 NG2 的表达进行 Western 印迹分析.
NG2 抗体 (LHM 2): sc-53389. 对 SK-MEL-24 全细胞裂解液中 NG2 的表达进行 Western 印迹分析.

NG2 抗体 (LHM 2): sc-53389

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说明书
  • NG2 抗体 LHM 2 是小鼠单克隆 IgG1 κ，NG2抗体, 在23篇文献中引用，规格为200 µg/ml
  • 针对human来源的A375P细胞粗提物
  • NG2 抗体 (LHM 2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 NG2
  • 抗NG2抗体（LHM 2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取NG2 (LHM 2): sc-53389的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 NG2 抗体 (LHM 2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与NG2 抗体 (LHM 2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

NG2抗体（LHM 2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠、大鼠和人类样本中的NG2蛋白。NG2 (LHM 2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 选项。NG2，也称为黑色素瘤相关硫酸软骨素蛋白多糖4，在黑色素瘤细胞早期在血管内皮基底膜上扩散时稳定细胞与基底膜的相互作用。这种稳定作用通过增强对肿瘤侵袭和生长至关重要的信号通路的激活，促进原发性黑色素瘤的进展。大鼠NG2中苏氨酸2256（或人NG2中苏氨酸2252）的磷酸化会触发星形细胞瘤表面的重新分布、细胞极化和细胞运动性的增加。NG2在恶性黑色素瘤细胞中作为共受体，促进细胞扩散和与α4β1整合素的局灶性接触形成。NG2出现在整个大鼠胚胎的血管和中央神经系统微血管的内皮细胞上，同时作为表皮干细胞的新标记，有助于它们的图案分布和聚集。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

NG2 抗体 (LHM 2) 参考文献:

  1. NG2基因敲除小鼠主动脉平滑肌细胞中的PDGF（α）受体对PDGF-AA无反应。  |  Grako, KA., et al. 1999. J Cell Sci. 112 (Pt 6): 905-15. PMID: 10036240
  2. 通过在黑色素瘤细胞上选择抗体噬菌体文库，分离人类肿瘤特异性抗体。  |  Kupsch, JM., et al. 1999. Clin Cancer Res. 5: 925-31. PMID: 10213230
  3. 表皮和毛囊祖细胞表达黑色素瘤缔合作用的硫酸软骨素蛋白多糖核心蛋白。  |  Ghali, L., et al. 2004. J Invest Dermatol. 122: 433-42. PMID: 15009727
  4. 室下区的NG2表达细胞是C型样细胞，有助于出生后海马的中间神经元生成。  |  Aguirre, AA., et al. 2004. J Cell Biol. 165: 575-89. PMID: 15159421
  5. NG2 蛋白聚糖通过参与 galectin-3 和 alpha3beta1 整合素促进内皮细胞运动和血管生成。  |  Fukushi, J., et al. 2004. Mol Biol Cell. 15: 3580-90. PMID: 15181153
  6. 蛋白激酶 C-α 对 NG2 蛋白聚糖的磷酸化调节极化膜分布和细胞运动。  |  Makagiansar, IT., et al. 2004. J Biol Chem. 279: 55262-70. PMID: 15504744
  7. 脑室下区 NG2 表达祖细胞的产后嗅球神经发生和神经胶质生成。  |  Aguirre, A. and Gallo, V. 2004. J Neurosci. 24: 10530-41. PMID: 15548668
  8. 血管生成素-2缺陷小鼠肾皮质管周毛细血管的畸形发育  |  Pitera, JE., et al. 2004. Am J Pathol. 165: 1895-906. PMID: 15579434
  9. 哺乳动物小脑中NG2-表达神经胶质的爬行纤维神经支配  |  Lin, SC., et al. 2005. Neuron. 46: 773-85. PMID: 15924863
  10. NG2 蛋白聚糖参与大鼠主动脉平滑肌细胞对血小板衍生物生长因子的反应。  |  Grako, KA. and Stallcup, WB. 1995. Exp Cell Res. 221: 231-40. PMID: 7589250
  11. 人类黑色素瘤细胞在黑色素瘤相关蛋白多糖（NG2）和α4 β1整合素刺激下的扩散和病灶接触形成。  |  Iida, J., et al. 1995. Cancer Res. 55: 2177-85. PMID: 7743521
  12. 针对人类黑色素瘤相关抗原的特异性单克隆抗体, 单链 Fv 和抗体融合噬菌体的生成与筛选。  |  Kupsch, JM., et al. 1995. Melanoma Res. 5: 403-11. PMID: 8589614

订购信息

产品名称产品编号规格价格数量收藏夹

NG2 抗体 (LHM 2)

sc-53389
200 µg/ml
$316.00

NG2 (LHM 2): m-IgG Fc BP-HRP 套装

sc-526805
200 µg Ab; 10 µg BP
$354.00

NG2 (LHM 2): m-IgG1 BP-HRP 套装

sc-532178
200 µg Ab; 20 µg BP
$354.00

NG2 抗体 (LHM 2) AC

sc-53389 AC
500 µg/ml, 25% agarose
$416.00

NG2 抗体 (LHM 2) HRP

sc-53389 HRP
200 µg/ml
$316.00

NG2 抗体 (LHM 2) FITC

sc-53389 FITC
200 µg/ml
$330.00

NG2 抗体 (LHM 2) PE

sc-53389 PE
200 µg/ml
$343.00

NG2 抗体 (LHM 2) Alexa Fluor® 488

sc-53389 AF488
200 µg/ml
$357.00

NG2 抗体 (LHM 2) Alexa Fluor® 546

sc-53389 AF546
200 µg/ml
$357.00

NG2 抗体 (LHM 2) Alexa Fluor® 594

sc-53389 AF594
200 µg/ml
$357.00

NG2 抗体 (LHM 2) Alexa Fluor® 647

sc-53389 AF647
200 µg/ml
$357.00

NG2 抗体 (LHM 2) Alexa Fluor® 680

sc-53389 AF680
200 µg/ml
$357.00

NG2 抗体 (LHM 2) Alexa Fluor® 790

sc-53389 AF790
200 µg/ml
$357.00

My question is about the use of NG2 Ab ( sc-53389) in flow cytometry. I wonder which protocol we should use, cell surface marker staining or intracellular staining. If you could give any information about it, I would appreciate it very much.

Asked by: NWNV21
Our FCM protocol is available online. For additional questions please contact Technical Service. https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Tech Sales
Date published: 2021-04-29

Hi, I used this antibody (1:500 dilution) for WB by testing on SK-MEL-28 and Hela under a reducing condition. I used Nupage gel Tris-acetate 3-8% and transferd to PVDF at 70V for 3h. I could not get any band. Could you please suggest solutions? Thanks!

Asked by: Jane Wang
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Service 11
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-11
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Rated 1 out of 5 by from Western BlotI used this antibody it for WB analysis at 1:200 dilution. Unfortunately it didn't detect any band of mouse origin. I will try it again for sure on different lysates.
Date published: 2020-10-06
Rated 1 out of 5 by from Did not work for western blotUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No bands were observed.
Date published: 2017-11-30
Rated 4 out of 5 by from wb还行数月前申请过小样，拿来做western blot，效果可以，免疫组化和荧光还没有尝试过，可以继续探索
Date published: 2017-05-11
Rated 5 out of 5 by from Outstanding !!Great western blot data, strong, clear signal; also nice IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Great IHC dataThe NG2 Antibody (LHM 2): sc-53389 provides excellent staining data for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification!
Date published: 2017-01-13
Rated 3 out of 5 by from Non-specificity in IHCThe antibody used in IHC gives robust non-specificity cytoplasmic background in thyroid cancer of mice.
Date published: 2016-12-06
Rated 1 out of 5 by from Rating really negative 1Rating really negative 1. Tested using tris and citrate retrieval on paraffin embedded mouse lung and it did NOT WORK AT ALL. Money wasted.
Date published: 2016-07-11
Rated 4 out of 5 by from Good specificity in WB in SKGood specificity in WB in SK-MEL-24 whole cell lysate. -SCBT QC
Date published: 2015-05-16
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NG2 抗体 (LHM 2) is rated 3.1 out of 5 by 9.
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