NF-H Antibody (D-9): sc-137009

NF-H Antibody (D-9) is a mouse monoclonal IgG1 antibody that detects neurofilament-H (NF-H) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NF-H is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining neuronal structural integrity. NF-H primarily localizes in the axonal cytoplasm, contributing to neurofilament formation essential for proper neuronal function. Neurofilaments regulate axon caliber, which affects nerve impulse conduction speed. NF-H undergoes extensive post-translational modifications, particularly phosphorylation, influencing interactions with other cytoskeletal elements and signaling pathways. Dysregulation of NF-H phosphorylation has been implicated in various neurodegenerative diseases, such as amyotrophic lateral sclerosis, Parkinson′s disease, and Alzheimer′s disease, highlighting NF-H importance in both neuronal health and disease. Anti-NF-H antibody (D-9) provides a valuable tool for researchers studying neurofilament assembly dynamics and implications in neurodegenerative conditions.

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NF-H Antibody (D-9) References:

1. Cyclin dependent kinase-5 (CDK-5) phosphorylates neurofilament heavy (NF-H) chain to generate epitopes for antibodies that label neurofilament accumulations in amyotrophic lateral sclerosis (ALS) and is present in affected motor neurones in ALS.  |  Bajaj, NP., et al. 1999. Prog Neuropsychopharmacol Biol Psychiatry. 23: 833-50. PMID: 10509378
2. Electrophysiological properties of axons in mice lacking neurofilament subunit genes: disparity between conduction velocity and axon diameter in absence of NF-H.  |  Kriz, J., et al. 2000. Brain Res. 885: 32-44. PMID: 11121527
3. Detection of neurofilament-H in serum as a diagnostic tool to predict injury severity in patients who have suffered mild traumatic brain injury.  |  Gatson, JW., et al. 2014. J Neurosurg. 121: 1232-8. PMID: 25192482
4. Effect of amitriptyline treatment on neurofilament-H protein in an experimental model of depression.  |  Sanna, MD., et al. 2017. Brain Res Bull. 128: 1-6. PMID: 27818278
5. Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22.  |  Lieberburg, I., et al. 1989. Proc Natl Acad Sci U S A. 86: 2463-7. PMID: 2928342
6. The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it.  |  Lees, JF., et al. 1988. EMBO J. 7: 1947-55. PMID: 3138108
7. Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury.  |  Bohnert, S., et al. 2021. Int J Legal Med. 135: 1525-1535. PMID: 33895854
8. Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.  |  Hisanaga, S., et al. 1994. Mol Biol Cell. 5: 161-72. PMID: 8019002
9. Phosphorylation and dephosphorylation of distinct isoforms of the heavy neurofilament protein NF-H.  |  Chertoff, R., et al. 1995. Cell Mol Neurobiol. 15: 269-81. PMID: 8590456
10. Isolation and characterization of the highly phosphorylated repeat domain of distinct heavy neurofilament subunit (NF-H) isoforms.  |  Soussan, L., et al. 1996. Cell Mol Neurobiol. 16: 463-77. PMID: 8879749

The preferred NF-H antibody is NF-H Antibody (RNF402): sc-32729.