Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nek2 CRISPR/Cas9 KO Plasmid (m): sc-421853
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nek2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nek2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nek2 Antibody (D-8): sc-55601
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nek2 CRISPR/Cas9 KO Plasmid (m) | sc-421853 | 20 µg | $397.00 |
Overview
Nek2 (NIMA related kinase 2) is a serine/threonine kinase that localizes to centrosomes and regulates centrosome separation, spindle assembly, and faithful chromosome segregation during mitosis. In mouse cells, Nek2 activity coordinates cell-cycle progression by phosphorylating centrosomal substrates and modulating microtubule dynamics, contributing to genome stability. Dysregulated Nek2 signaling has been associated with centrosome amplification, aneuploidy, and altered proliferative control, making it relevant to studies of tumor biology and mitotic checkpoint integrity. Nek2 also intersects with pathways governing DNA damage responses and ciliary dynamics, supporting its use as a node for dissecting cell division–linked signaling networks.
Nek2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nek2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nek2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nek2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nek2 protein expression.
This CRISPR knockout system enables efficient generation of Nek2-deficient cell models for investigation of Nek2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nek2 exon(s) critical for Nek2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nek2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nek2 CRISPR/Cas9 KO Plasmid (m) and Nek2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nek2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nek2 HDR Plasmid (m) and Nek2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nek2 homology arms to support homology-directed repair at defined Nek2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.