NanogNB CRISPR/Cas9 KO Plasmid (h): sc-418788
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NanogNB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NanogNB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NanogNB CRISPR/Cas9 KO Plasmid (h) | sc-418788 | 20 µg | $397.00 |
Overview
NANOGNB (NanogNB) is a human NANOG-related gene implicated in regulatory programs linked to pluripotency and early developmental gene expression. As a paralog/related factor to core stemness circuitry, NanogNB is studied in the context of transcriptional control, cell fate specification, and epigenetic state maintenance, with potential crosstalk to pathways that govern self-renewal and differentiation. Altered expression of NANOG-family genes is frequently evaluated in models of tumor cell plasticity, germ cell biology, and reprogramming, where shifts in stem-like transcriptional networks can influence proliferation and lineage commitment. NANOGNB therefore serves as a target for dissecting how NANOG-associated regulatory architecture contributes to developmental processes and disease-relevant cellular phenotypes.
NanogNB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NANOGNB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NANOGNB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NANOGNB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NanogNB protein expression.
This CRISPR knockout system enables efficient generation of NANOGNB-deficient cell models for investigation of NanogNB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NANOGNB exon(s) critical for NanogNB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NANOGNB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NanogNB CRISPR/Cas9 KO Plasmid (h) and NanogNB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NANOGNB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NanogNB HDR Plasmid (h) and NanogNB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NANOGNB homology arms to support homology-directed repair at defined NANOGNB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.