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beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) - Western Blotting - Image 381808
Na+/K+-ATPase β1抗体 (C464. 8): sc-21713. C6 (A) 和 Neuro-2A (B) 全细胞裂解液及大鼠脑组织提取物 (C) 中 Na+/K+-ATPase β1 表达的 Western 印迹分析.
Na+/K+-ATPase β1抗体 (C464. 8): sc-21713. C6 (A) 和 Neuro-2A (B) 全细胞裂解液及大鼠脑组织提取物 (C) 中 Na+/K+-ATPase β1 表达的 Western 印迹分析.

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8): sc-21713

4.6(5)
寫評論提問

说明书
  • beta 1 Sodium Potassium ATPase/ATP1B1 抗体 C464.8 是小鼠单克隆 IgG2a κ，beta 1 Sodium Potassium ATPase/ATP1B1抗体, 在25篇文献中引用，规格为200 µg/ml
  • 纯化的rabbit肾外髓质，表位图谱与β1亚基的外部域
  • beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) 推荐用于 WB, IP 和 IF，检测mouse, rat, human, rabbit 和canine 来源的 Na+/K+-ATPase β1
  • 抗beta 1 Sodium Potassium ATPase/ATP1B1抗体（C464.8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta 1 Sodium Potassium ATPase/ATP1B1 (C464.8): sc-21713的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

额外Sodium/Potassium-ATPase Protein抗体，包括H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3和Na+/K+-ATPase α 1

Na+/K+-ATPase β1 抗体（C464.8）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠、人类、兔子和犬的 Na+/K+-ATPase β1 蛋白。Na+/K+-ATPase β1 (C464.8) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Na+/K+-ATPase beta 1蛋白在Na+/K+-ATPase复合体的正常运作中起着至关重要的作用，而Na+/K+-ATPase复合体对于维持细胞内环境平衡至关重要。Na+/K+-ATPase beta 1主要位于细胞质膜中，通过促进钠离子和钾离子跨膜的主动转运，参与离子梯度的调节。Na+/K+-ATPase beta 1的正确定位确保了Na+/K+-ATPase泵的高效运转，这对于维持静息膜电位、调节细胞体积和支持各种细胞信号通路等过程至关重要。Na+/K+-ATP酶复合物中的β亚基和α亚基之间的相互作用尤为重要，因为β亚基有助于α亚基正确折叠并插入膜中，同时影响泵的活性。抗Na+/K+-ATP酶β1抗体（C464.8）是研究人员研究这种重要蛋白在各种生物学环境中的生理和病理作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) 参考文献:

  1. Na,K-ATPase gamma 亚基的 gamma a 和 gamma b 形式的功能作用和免疫细胞化学定位。  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
  2. 化学修饰是阐明钠泵结构与功能关系的一种方法。  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
  3. 钠泵需要其 beta 亚基。  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
  4. Na,K-ATPaseα-亚基和β-亚基在正确的翻译后处理和细胞内转运方面相互依赖。  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
  5. 一种与 Na,K-ATP 酶相关的蛋白脂质对 ATP 酶的活性并非必不可少。  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
  6. 异源二聚体 P 型 ATP 酶 beta 亚基的功能意义。  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
  7. Na+,K+ -ATP酶。  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
  8. 在酵母细胞中表达功能性钠泵的亚基要求。  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
  9. Na,K-ATP 酶的α-亚基具有独立于β-亚基的催化活性。  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
  10. 在杆状病毒感染的昆虫细胞中表达, 定位和组装功能性 Na,K-ATP 酶多肽。  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
  11. 与 Na,K-ATP 酶相关的小蛋白--γ 多肽的分子克隆和免疫学特征。  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
  12. Na,K 泵的结构和功能：分子生物学十年。  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

订购信息

产品名称产品编号规格价格数量收藏夹

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8)

sc-21713
200 µg/ml
$316.00

beta 1 Sodium Potassium ATPase/ATP1B1 (C464.8): m-IgG Fc BP-HRP 套装

sc-525500
200 µg Ab; 10 µg BP
$354.00

beta 1 Sodium Potassium ATPase/ATP1B1 (C464.8): m-IgG2a BP-HRP 套装

sc-546292
200 µg Ab; 10 µg BP
$354.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) AC

sc-21713 AC
500 µg/ml, 25% agarose
$416.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) HRP

sc-21713 HRP
200 µg/ml
$316.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) FITC

sc-21713 FITC
200 µg/ml
$330.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) PE

sc-21713 PE
200 µg/ml
$343.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 488

sc-21713 AF488
200 µg/ml
$357.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 546

sc-21713 AF546
200 µg/ml
$357.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 594

sc-21713 AF594
200 µg/ml
$357.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 647

sc-21713 AF647
200 µg/ml
$357.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 680

sc-21713 AF680
200 µg/ml
$357.00

beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) Alexa Fluor® 790

sc-21713 AF790
200 µg/ml
$357.00

I am using the primary antibody (ATP1B1) conjugate agarose SC-21713AC for Immunoprecipitation, but I got nothing in the IP result, and the protein is still in the flowtrough. how to deal with this case? thanks.

Asked by: gtdghd
Thank you for your question. Please call or email technical service . (800)-457-3801 or scbt@scbt.com
Answered by: BlakeJ
Date published: 2023-07-11

What blocking solution should I use with Na+/K+-ATPase β1 (C464.8): sc-21713 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Works in porcine cells for blots.Gives a band of the proper size on western blot when blotting lysate obtained from pig kidney cells.
Date published: 2018-04-06
Rated 5 out of 5 by from Produced a lot of publishable dataThis antibody was featured in over 20 publications in the primary literature for the use of WB, IP, IF and IHC on m, r, h, rabbit, canine samples
Date published: 2017-01-18
Rated 5 out of 5 by from Worked great in WB in mouse cellsWorked great in WB in mouse cells.
Date published: 2015-06-29
Rated 4 out of 5 by from Positive signal achieved in mouse brain tissuePositive signal achieved in mouse brain tissue extract. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Robust blot obtained in WB against MDCK wholeRobust blot obtained in WB against MDCK whole cell lysate. -SCBT QC
Date published: 2013-11-05
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beta 1 Sodium Potassium ATPase/ATP1B1 抗体 (C464.8) is rated 4.6 out of 5 by 5.
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