Date published: 2025-9-6
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alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) - Western Blotting - Image 351984
Na+/K+-ATPase α3 抗体 (H-4): sc-365744. 大鼠大脑 (A) 和大鼠小脑 (B) 组织提取物中 Na+/K+-ATPase α3 表达的 Western 印迹分析.
Na+/K+-ATPase α3 抗体 (H-4): sc-365744. 大鼠大脑 (A) 和大鼠小脑 (B) 组织提取物中 Na+/K+-ATPase α3 表达的 Western 印迹分析.

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4): sc-365744

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说明书
  • alpha 3 Sodium Potassium ATPase/ATP1A3抗体(H-4)是小鼠单克隆IgG1 κ, 在5篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Na+/K+- ATPase α3的内部区域内的氨基酸419-446之间
  • 抗-alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Na+/K+-ATPase α3; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 抗alpha 3 Sodium Potassium ATPase/ATP1A3抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha 3 Sodium Potassium ATPase/ATP1A3 (H-4): sc-365744的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

附加Sodium/Potassium-ATPase Protein抗体，包括H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3和Na+/K+-ATPase α 1

α3 Sodium Potassium ATPase/ATP1A3抗体（H-4）是一种IgG1 κ型小鼠单克隆Na+/K+-ATPase α3抗体（也被称作Na+/K+-ATPase α3抗体），能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测源自小鼠、大鼠和人类的Na+/K+-ATPase α3。α3 Sodium Potassium ATPase/ATP1A3抗体（H-4）既可作为非偶联型Na+/K+-ATPase α3抗体提供，也可作为多种偶联型Na+/K+-ATPase α3抗体形式存在，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。普遍表达的钠/钾-ATP酶（Na+/K+-ATPase）以寡聚体形式存在于质膜复合物中，该复合物将一个ATP分子的水解与三个Na+离子和两个K+离子逆着它们各自的电化学梯度运输相耦合。作为P型离子动机家族的成员，Na+/K+-ATPase在维持细胞体积、静息膜电位和Na+偶联溶质转运中起着关键作用。Na+/K+-ATPase寡聚体由三个亚基（α、β和γ）的多种异构体组成。α亚基包含ATP和阳离子的结合位点；糖基化的β亚基确保α亚基的正确折叠和膜插入。小的γ亚基在肾小管区段中与α亚基共同定位，从而提高Na+/K+-ATPase对ATP的亲和力。β亚基而非γ亚基对于Na+/K+-ATPase的正常活性至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) 参考文献:

  1. Na,K-ATPase gamma 亚基的 gamma a 和 gamma b 形式的功能作用和免疫细胞化学定位。  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
  2. 化学修饰是阐明钠泵结构与功能关系的一种方法。  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
  3. 钠泵需要其 beta 亚基。  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
  4. Na,K-ATPaseα-亚基和β-亚基在正确的翻译后处理和细胞内转运方面相互依赖。  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
  5. 一种与 Na,K-ATP 酶相关的蛋白脂质对 ATP 酶的活性并非必不可少。  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
  6. 异源二聚体 P 型 ATP 酶 beta 亚基的功能意义。  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
  7. Na+,K+ -ATP酶。  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
  8. 在酵母细胞中表达功能性钠泵的亚基要求。  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
  9. Na,K-ATP 酶的α-亚基具有独立于β-亚基的催化活性。  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
  10. 在杆状病毒感染的昆虫细胞中表达, 定位和组装功能性 Na,K-ATP 酶多肽。  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
  11. 与 Na,K-ATP 酶相关的小蛋白--γ 多肽的分子克隆和免疫学特征。  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
  12. Na,K 泵的结构和功能：分子生物学十年。  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

订购信息

产品名称产品编号规格价格数量收藏夹

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4)

sc-365744
200 µg/ml
$316.00

alpha 3 Sodium Potassium ATPase/ATP1A3 (H-4): m-IgG1 BP-HRP 套装

sc-531709
200 µg Ab; 20 µg BP
$354.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) AC

sc-365744 AC
500 µg/ml, 25% agarose
$416.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) HRP

sc-365744 HRP
200 µg/ml
$316.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) FITC

sc-365744 FITC
200 µg/ml
$330.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) PE

sc-365744 PE
200 µg/ml
$343.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 488

sc-365744 AF488
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 546

sc-365744 AF546
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 594

sc-365744 AF594
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 647

sc-365744 AF647
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 680

sc-365744 AF680
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) Alexa Fluor® 790

sc-365744 AF790
200 µg/ml
$357.00

alpha 3 Sodium Potassium ATPase/ATP1A3 (H-4) 中和勝肽

sc-365744 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365744 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Weak BandsI tried different concentrations of antibody in WB experiments. However, the bands were too weak and difficult to quantify.
Date published: 2018-04-08
Rated 5 out of 5 by from Works well on blotsWe compared the H-4 Na,K-ATPase alpha3 antibody with the old XVI-F9G10 monoclonal, side by side. We diluted the F9G10 ascites 1:750,000 as we usually do. We diluted the H-4 antibody 1:500 (more dilute than the 1:200 recommended). The blot was of a crude homogenate of mouse brain, and both blots were almost clean of background bands. Although we did not yet test it on tissue that has alpha1 or 2 and not alpha3, brain has all three isoforms, and we did not detect anything suspicious of crossreactivity. We have not yet tested it on tissue.
Date published: 2016-12-15
Rated 5 out of 5 by from Produced positive Western blot data of Na+/K+Produced positive Western blot data of Na+/K+-ATPase 3 expression in rat brain tissue extract. -SCBT QC
Date published: 2014-03-15
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alpha 3 Sodium Potassium ATPase/ATP1A3 抗体 (H-4) is rated 4.0 out of 5 by 3.
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